Asian Pacific Journal of Tropical Biomedicine
The inhibitory effect of dihydroartemisinin on the growth of neuroblastoma cells
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Supported by the National Natural Science Foundation of China (NO. 81201671, NO. 81202953 and NO. 81202031).

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    Abstract:

    Objective: To evaluate the inhibitory effect of dihydroartemisinin on neuroblastoma cell line SH-SY5Y, explore the possible mechanism of dihydroartemisinin against neuroblastoma cells. Methods: The cell viability of dihydroartemisinin treated SH-SY5Y cells was examined by MTT assay and morphology of cells was observed by using inverted microscope. Cell cycle was examined with flowcytometry assay, then cyclin D1 and caspase-3 proteins expression was detected by ELISA and western blotting assay. Results: MTT analysis results showed that cell viability significantly decreased after exposure to 0.05, 0.50, 5.00 and 50.00 μmol/L dihydroartemisinin in a dose-dependent manner, and the lower density of cells was observed in treated groups. The number of cells in sub-G1 phase was increased after treatment with different doses of dihydroartemisinin compared with the control group. The expression of cyclin D1 protein was decreased, while the expression of caspase-3 protein was increased in treated group. Conclusions: Dihydroartemisinin could inhibit the proliferation through stopping the cell cycle and inducing the apoptosis in neuroblastoma SH-SY5Y cells.

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Ling Qi,Yang Yang,Yu-Cui Liu,Tian-Xin Zhu, Song Jin, Lin Zang,et al. The inhibitory effect of dihydroartemisinin on the growth of neuroblastoma cells. Asian Pac J Trop Biomed 2016; 6(4): 279-282.

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History
  • Received:October 20,2015
  • Revised:November 01,2015
  • Adopted:January 13,2016
  • Online: March 08,2016
  • Published:

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