Objective: To examine the chemical component of the essential oil of Eremostachys laciniata and evaluate antioxidant activity of the extract. Methods: The hydrodistillated essential oil was analyzed by gas chromatography-flame ionization detector and gas chromatography–mass spectrometry. Also, antioxidant activity, total phenolic and flavonoid content were determined by 2,2-diphenyl-1- picrylhydrazyl, Folin-Ciocalteu and colorimetric method, respectively. Results: The major components of the essential oil were p-cymene (21.64%), linalool (17.93%), and α-pinene (11.42%). Also, the extract obtained by methanol showed a good antioxidant activity. The same extract also exhibited high phenolic and flavonoid contents. Conclusions: These results indicate that Eremostachys laciniata can be used in dietary applications with a potential to reduce oxidative stress.