Asian Pacific Journal of Tropical Medicine

Volume 7,Issue 5,2014 Table of Contents

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  • 1  Inhibitory effect of humanized anti-VEGFR-2 ScFv-As2O3-stealth nanoparticles conjugate on growth of human hepatocellular carcinoma: in vitro and in vivo studies
    Xiang-Bao Yin Lin-Quan Wu Hua-Qun Fu Ming-Wen Huang Kai Wang Fan Zhou Xin Yu Kai-Yang Wang
    2014, 7(5):337-343. DOI: 10.1016/S1995-7645(14)60052-3
    [Abstract](31) [HTML](0) [PDF 1.85 M](121)
    Abstract:
    Objective: To investigate the inhibitory effect of humanized anti-VEGFR-2 ScFv-As2O3-stealth nanoparticles conjugate on growth of human hepatocellular carcinoma both in vitro and in vivo, which may be a potential agents with sensitivity and targeting ability for human hepatocellular cancer. Methods: Humanized anti-VEGFR-2 ScFv-As2O3-stealth nanoparticles conjugate was previously constructed using ribosome display technology and antibody conjugate technology. In this combined in vitro and in vivo study, the inhibitory effects of anti-VEGFR-2 ScFv-As2O3-stealth nanoparticles conjugate on tumor growth, invasion, and metastasis was observed with human liver carcinoma cell line Bel7402 and normal cell L02 by MTT assay, Tanswell assay, Hochest33258 staining, and DNA ladder analysis. The anticancer activity and distribution of anti-VEGFR-2 ScFv-As2O3-stealth nanoparticles was then verified in a mouse model of Bel7402 xenografts. Results :Anti-VEGFR-2 ScFv-As2O3-stealth nanoparticles significantly inhibited the proliferation of Bel7402 in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay while had almost no effects on L02 cells. And the apoptosis inducing effects were proved by Hochest33258 staining and DNA ladder analysis. Transwell assay found that the drug also inhibited the metastasis ability of tumor cells. Furthermore, anti-VEGFR-2 ScFv-As2O3-stealth nanoparticles significantly delayed the growth of Bel7402 xenografts after administration (92.9%), followed by As2O3-stealth nanoparticles, anti-VEGFR-2 ScFv, and As2O3 (61.4%, 58.8%, 20.5%, P<0.05). The concentration of As2O3 in anti-VEGFR-2 ScFv-As2O3-stealth nanoparticles group was more selectively. Conclusions: Anti-VEGFR-2 ScFv-As2O3-stealth nanoparticles is a potent and selective anti-hepatocellular carcinoma agent which could inhibit the growth of liver cancer as a targeting agent both in vitro and in vivo and also significantly inhibit angiogenesis.
    2  Gene expression profiles associated with osteoblasts differentiated from bone marrow stromal cells
    Lu Lu Yang Gao Miao Xu Ru-Cun Ge Lin Lu
    2014, 7(5):344-351. DOI: /10.1016/S1995-7645(14)60053-5
    [Abstract](27) [HTML](0) [PDF 5.87 M](201)
    Abstract:
    Objective:To study the changes of gene expression profiles associated with osteoblasts differentiated from rat bone marrow stromal cells in vitro by gene chip technique. Methods:rat Bone marrow stromal cells were isolated and cultured, and differentiation was induced by dexamethasone, β-glycerol phosphate and vitamin C. Cellular mRNA was extracted and reverse transcribed into cDNA, thus related genes expression differences were detected by gene expression profile chip. Results:Calcifying nodules were visible in the induced cells. There were 27.7% genes expressed differentially, three times more than the normal and induced cells, and some genes were related to transcription, translation, glycosylation modification. Extracellular matrix, signal molecules and metabolism were up-regulated. Conclusions:The gene chip technique can be used to detect the multi-gene different expression in the differentiation-induceed rat BMSCs, and these differentially expressed genes are necessary genes related to rat BMSCs proliferation and induction of osteoblastic differentiation.
    3  Effects of ultrasound-combined microbubbles on hippocampal AchE fibers in rats
    Zi-Li Gong Chun-Mei Luo Sheng-Zheng Wu Hong Ran Jie Zhu Jian Zheng
    2014, 7(5):352-357. DOI: /10.1016/S1995-7645(14)60054-7
    [Abstract](30) [HTML](0) [PDF 2.80 M](165)
    Abstract:
    Objective:To investigate the protective effect of ultrasound-combined microbubbles on hippocampal acetylcholinesterase (AchE) fibers in rats. Methods:According to random digits table, 60 SD rats were divided into two groups, marrow stromal cells (MSCs) intracranial transplantation group and MSCs intracranial transplantation + ultrasonic microbubbles group. Marrow stromal cells were cultivated and isolated in vitro; 12 weeks after transplantation, spatial learning and memorizing abilities of rats were assessed by Morris water maze; AchE staining method was used to observe changes in density and appearance of AchE staining positive fibers in hippocampal CA1 region. Results:There was asignificant increase in spatial learning and memorizing abilities of rats in MSCs intracranial transplantation + ultrasonic microbubbles group. Hippocampal AchE staining suggested an increase in the density of AchE staining positive fibers in MSCs intracranial transplantation group; the fibers were regular, intact and dense. Density of hippocampal AchE positive fibers was negatively correlated with the escape latent period and was positively correlated with percentage of the time needed to cross each platform quadrant. Conclusions:Better promotion of spatial learning and memorizing abilities of rats in MSCs intracranial transplantation + ultrasonic microbubbles group may be related with the protective effect of ultrasound-combined microbubbles on hippocampal acetylcholine fibers.
    4  Decreased proliferation ability and differentiation potential of mesenchymal stem cells of osteoporosis rat
    Qiang Wang Bing Zhao Chao Li Jie-Sheng Rong Shu-Qing Tao Tian-Zun Tao
    2014, 7(5):358-363. DOI: 10.1016/S1995-7645(14)60055-9
    [Abstract](11) [HTML](0) [PDF 1.73 M](193)
    Abstract:
    Objective: To explore decreased proliferation ability and differentiation potential of mesenchymal stem cells (MSCs) of osteoporosis rat. Methods: MSCs were obtained from osteoporosis rat, and proliferation potency and impaired osteogenic differentiation potential were determined. Results: The result showed a significant downregulation of MSCs pluripotency related gene (Oct 4) and osteogenic genes (BSP, OCN) expression in OVX MSCs compared with Sham MSCs (P<0.05). Conclusions: These data suggest that MSCs are aging in osteoporosis body, and autologous OVX MSCs transplantation is not appropriate to treat osteoporosis if necessary. There will be a possibility in establishing a new clinical application of MSCs autologous transplantation to treat osteoporosis, if OVX MSCs have stronger proliferation and differentiation.
    5  Effects of MicroRNA-10b on lung cancer cell proliferation and invasive metastasis and the underlying mechanism
    Qiao-Li Su Shuang-Qing Li Duo-Ning Wang Feng Liu Bo Yuan
    2014, 7(5):364-367. DOI: /10.1016/S1995-7645(14)60056-0
    [Abstract](30) [HTML](0) [PDF 1011.60 K](161)
    Abstract:
    Objective:To study the influence of MicroRNA-10b on proliferation and invasion of human low metastatic lung cancer cell 95-C and its mechanism. Methods:Lipofectamine MicroRNA-10b eukaryotic expression plasmid was transfected into 95-C. The experiment group was divided into blank control group, empty vector transfected group and MicroRNA-10b transfected group. Real time quantitative RT-PCR was used to detect the expression of MicroRNA-10b and KLF4mRNA expression. Proliferations of cells were detected by cell proliferation assay, invasion of the detected the cell Transwell experiments, the expression of KLF4 protein was detected in Western blotting cells. Results:The proliferation rate of MicroRNA-10b plasmid transfection group increased significantly after transfection, invasion and migration ability enhancement, by comparison, there are statistically significant differences in the blank control group and negative control group (P<0.05); the expression of MicroRNA-10b plasmid transfection group KLF4 protein decreased, the difference was statistically significant (P<0.05); reduce the expression of MicroRNA-10b plasmid transfection group KLF4mRNA, but no significant differences (P>0.05). Conclusions:MicroRNA-10b may promote proliferation and invasion of 95-C cells by down regulating the expression of KLF4 protein.
    6  Effects of mTOR-STAT3 on the migra=tion and invasion abilities of hepatoma cell and mTOR-STAT3 expression in liver cancer
    Xia Pu Qing-Xi Guo Han-An Long Cheng-Wan Yang
    2014, 7(5):368-372. DOI: 10.1016/S1995-7645(14)60057-2
    [Abstract](38) [HTML](0) [PDF 1.12 M](128)
    Abstract:
    Objective: To investigate the effects of mTOR-STAT3 pathway on the invasion and migration of hepatoma cell. Methods: mTOR and STAT3 expresssion in the hepatocellular carcinoma cell line HepG2 and normal liver cell line L02 were detected by reverse transcription PCR (RT-PCR) and western blotting. The migration and invasion abilities of cells and expression of STAT3 were detected by scratch adhesion test and transwell migration assays, after siRNA transfection blocking mTOR expression of HepG2 cells. Results: The HepG2 cells expression is higher compared with normal cells L02 expression. Western blotting assay showed the mTOR expression was blocked, while STAT3 expression was also decreased, after the siRNA transfection of HepG2 cells. The migration (scratch adhesion test) and invasion (transwell assays) abilities of HepG2 cells which the mTOR expression was blocked by siRNA interference were significantly decreased (P<0.05). Conclusion: mTORSTAT3 expression in hepatoma cells HepG2 was significantly higher than that in normal liver cells. mTOR blocking can reduce the expression of STAT3, which is also closely related to the invasion and metastasis of liver cancer cells.
    7  Silence of STIM1 attenua=tes the proliferation and migration of EPCs after vascular injury and its mechanism
    Xin-Peng Cong Wen-Hui Wang Xi Zhu Can Jin Liang Liu Xin-Min Li
    2014, 7(5):373-377. DOI: /10.1016/S1995-7645(14)60058-4
    [Abstract](24) [HTML](0) [PDF 1.18 M](134)
    Abstract:
    Objective: To investigate the effect of stromal interaction molecule 1(STIM1) knockdown on the proliferation and migration of endothelial progenitor cells (EPCs) after vascular injury and its mechanism. Methods: The rat bone marrow derived EPCs were divided into three groups: adenovirus negative control (group NSC), rat STIM1 adenovirus vector transfection group (group si/rSTIM1) and rat &human recombinant STIM1 adenovirus transfection group (group si/rSTIM1+hSTIM1). The STIM1 expressions in each group were detected by reverse transcription PCR after transfection; the cell proliferation was tested by [3H] thymidine incorporation assay (3H-TdR); Cell cycle was analyzed by flow cytometry; the cells' migration activity was detected by Boyden assay; Calcium ion concentration was detected by using laser confocal method. Results: 48 h later after transfection, the expression level of STIM1 in si/rSTIM1 cells was significantly lower than that in NSC group (0.21±0.12 vs 1.01±0.01, P<0.05); EPCs that stayed in G1 phase in si/rSTIM1 group [(93.31±0.24)%] were significantly more than that in NSC group [(78.03±0.34)%, P<0.05]; EPCs' migration activity in si/rSTIM1 group (10.03±0.33) was significantly lower than that in NSC group: (32.11±0.54, P<0.05); EPCs calcium ion concentration changes in EPCs in si/rSTIM1 group (38.03±0.13) was significantly lower than that in NSC group (98.11±0.34, P<0.05). While there was no significant difference between si/rSTIM1+hSTIM1 group and NSC group on the four indexes above. Conclusions: Silence of STIM1 attenuates EPCs proliferation and migration after vascular injury, by mediating the calcium ion concentration in EPCs.
    8  Effect of Yiqi Jianpi plus anticancer herbs on spleen deficiency in colorectal cancer and its anti-tumor role
    Li-Ran Fu Sheng-Wei Guo Xian-Hui Liu
    2014, 7(5):378-381. DOI: 10.1016/S1995-7645(14)60059-6
    [Abstract](22) [HTML](0) [PDF 1.97 M](157)
    Abstract:
    Objective: To observe the effect of Yiqi Jianpi plus anticancer herbs on spleen deficiency in colorectal cancer and its anti-tumor role.Methods: Human intestinal cancer cell HT29 xenograft of nude mice model was established. The expression of EGF, VEGF, gastric cancer tumor growth in mice were observed. Results: Protein kinase C expression in in the Yiqi Jianpi group and Yiqi Jianpi anti-tumor group was significantly better than the model group (P<0.01, P<0.05). There was significantly more apoptotic cells in Yiqi Jianpi anti-tumor group than Yiqi Jianpi group and model group (P<0.01). Epidermal growth factor and vascular endothelial growth factor expression in Yiqi Jianpi group was significantly lower than Yiqi Jianpi group and model group (P<0.05). Conclusions: Tumor can inhibit the expression of PKC inhibition. Yiqi Jianpi and anticancer treatment can reduce this inhibition. Besides this treatment can also inhibit expression of tumor related genes such as epidermal growth factor and vascular endothelial growth factor.
    9  Reinforcing effect of calcium sulfate cement bovine bone morphogenetic protein on vertebral in the rabbit model of osteoporosis
    Jie Zhang Sheng-Guo Chen Kaken Habaerxi Shawuti Alimujiang Yu Chen Ming-Zhen Peng Rong Yue Yu-Lian Wu De-Quan Wang Yu-Ming Chen
    2014, 7(5):382-385. DOI: /10.1016/S1995-7645(14)60060-2
    [Abstract](26) [HTML](0) [PDF 1.24 M](176)
    Abstract:
    Objective: To observe reinforcing effect of calcium sulfate cement (CSC) bovine bone morphogenetic protein (bBMP) on vertebral in the rabbit model of osteoporosis. Methods: A total of 48 New Zealand white rabbits were randomly divided into group I (blank control group), group II (CSC injection group), group III (CSC/bBMP injection group) and control group. White rabbit osteoporosis model was established rapidly by using castration method+methylprednisolone candidate. After modeling, groups II, III were given corresponding vertebral body injection material, and 4 animals were sacrificed respectively at 24 h, 6 weeks, 12 weeks after vertebral plasty. Tissue pathological status, vertebral mineral density and vertebral body bone mechanical strength were observed. Results: Vertebral body structure form was normal in the groups II and III. Trabecular bone coarsens, connection and repair were observed in micro fracture and bone defects, bone trabecular connectivity was superior to group I significantly; vertebral body compression strength in the groupI was on the decline, vertebral compression strength in the groups IIand III was on the rise, the largest vertebra. Postoperative BMC and BMD in groups IIand III were incresed, and significantly higher than group I after 6 weeks (P<0.05), BMC and BMD in group III after 12 weeks were higher than the other three groups. Conclusions: Compound bBMP CSC has good bone induction. It can improve the three-dimensional construction effect for osteoporosis vertebral trabecula, and can significantly improve the vertebral strength, as a vertebral packing material with good application prospect.
    10  Expression of Bcl-2 and NF-κB in brain tissue after acute renal ischemia-reperfusion in rats
    Na Zhang Gen-Yang Cheng Xian-Zhi Liu Feng-Jiang Zhang
    2014, 7(5):386-389. DOI: /10.1016/S1995-7645(14)60061-4
    [Abstract](40) [HTML](0) [PDF 666.33 K](154)
    Abstract:
    Objective: To investigate the effect of acute renal ischemia reperfusion on brain tissue. Methods: Fourty eight rats were randomly divided into four groups (n=12): sham operation group, 30 min ischemia 60 min reperfusion group, 60 min ischemia 60 min reperfusion group, and 120 min ischemia 60 min reperfusion group. The brain tissues were taken after the experiment. TUNEL assay was used to detect the brain cell apoptosis, and western blot was used to detect the expression of apoptosis-related proteins and inflammatory factors. Results: Renal ischemia-reperfusion induced apoptosis of brain tissues, and the apoptosis increased with prolongation of ischemia time. The detection at the molecular level showed decreased Bcl-2 expression, increased Bax expression, upregulated expression of NF-κB and its downstream factor COX-2/PGE2. Conclusions: Acute renal ischemia-reperfusion can cause brain tissue damage, manifested as induced brain tissues apoptosis and inflammation activation.
    11  Effect of matrine on transforming growth factor β1 and hepatocyte growth factor in rat liver fibrosis model
    Jian-Lan Yu Jun-Hua Li Rong-Gui Chengz Yan-Mei Ma Xiao-Juan Wang Jing-Chun Liu
    2014, 7(5):390-393. DOI: /10.1016/S1995-7645(14)60062-6
    [Abstract](25) [HTML](0) [PDF 2.28 M](144)
    Abstract:
    Objective: To observe the preventive and control effect of matrine on transforming growth factor (TGF-β1) and hepatocyte growth factor (HGF) of liver fibrosis tissue in rats. Methods: A total of 48 SD rats were randomly divided into A, B, C, D groups with 12 in each, group A as the normal control group and groups B, C, D as liver fibrosis models using composite modulus method with carbon tetrachloride (CCL4). Group B was the model group, group C adopted γ-interferon lavage therapy in the second day of modeling, and group D adopted matrine lavage treatment, at 4 and 8 weeks after treatment. Six rats were executed for detection of TGF-β1 and HGF, liver tissue histology and comparison fibrosis degree changes of rat liver tissue between groups. Results: Groups B, C, D showed a more significantly increased TGF-β1 at each time point compared with group A (P<0.05); Group B showed a more significantly increased TGF-β11 than groups C and D at weeks 4 and 8 (P<0.05); group D showed a lowest level of TGF-β1, followed by groups C and B. HGF of group B decreased more significantly than A group at weeks 4 and 8 (P<0.05); HGF of groups C and D was significantly elevated at 4 and 8 weeks than groups A and B (P<0.05), in which the group D showed the highest level of HGF. According to tissue histologic observation, rat liver tissue structure of group A was clear and normal, tissue structure of group B was destroyed with obvious fibrous tissue hyperplasia and fatty change of hepatic cells; groups C and D showed a slighter liver tissue damage, cell necrosis and connective tissue hyperplasia in collect abbacy than group B with a trend of obvious improvement. Conclusions: Matrine can reduce TGF-β1 expression and enhance the activity of HGF, so as to realize the inhibition effect on liver fibrosis in rats.
    12  In vitro study on blocking mTOR signaling pathway in EGFR-TKI resistance NSCLC
    Xu-Dong Xiang Jing Yu Gao-Feng Li Lin Xie Heng Li
    2014, 7(5):394-397. DOI: 10.1016/S1995-7645(14)60063-8
    [Abstract](26) [HTML](0) [PDF 711.36 K](132)
    Abstract:
    Objective: To investigate the effect and mechanism of inhibitor everolimus on EGFR-TKI resistance NSCLC. Methods: MTT assay was used to detect proliferation of human non-small cell lung cancer cell line A549. Flow cytometry was used to detect the changes of apoptosis and cycle distribution in each group after 24 h and 48 h. RT-PCR was used to detect the changes of PTEN and 4EBP1 expression levels after 48 h of monotherapy and combination therapy. Results: MTT assay showed that everolimus had dose-dependent inhibition against growth of A549 cells. Flow cytometry showed when everolimus could induce apoptosis and induce G0/G1 phase cell cycle arrest, which was time-dependent (P<0.05). RT-PCR showed everolimus could increase PTEN and 4EBP1 expression. Conclusions: mTOR inhibitor everolimus has an inhibitory effect on EGFR-TKI resistant NSCLC, which cannot reverse the resistance effect of EGFR-TKI resistant cell line A549. The relationship between EGFR/AKT signaling pathway and the mTOR signaling pathway and the mechanism in non-small cell lung cancer need further study
    13  Effect of atorvastatin on serum oxidative stress and N-terminal brain natriuretic peptide expression in rats
    Yan Xu Yu Yang Ying-Quan Luo
    2014, 7(5):398-401. DOI: 10.1016/S1995-7645(14)60064-X
    [Abstract](12) [HTML](0) [PDF 440.86 K](122)
    Abstract:
    Objective: To investigate the effect of atorvastatin on serum oxidative stress and N-terminal brain natriuretic peptide expression in rats. Methods: A total of 40 healthy male SD rats were randomly divided into the sham group (Group A, n=10, saline 5 mL/d), ischemia-reperfusion group (Group B, n=10, saline 5 mL/d), atorvastatin group (Group C, n=10, atorvastatin 20 mg/ kg•d), atorvastatin + N-amino-arginine group (Group D, n=10, atorvastatin 20 mg/kg•d + N-amino arginine 15 mg/kg). Myocardial ischemia-reperfusion rat model was established after 3 days of gavage. N-amino arginine 15 mg/kg was given by tail vein injection 15 min before ischemia. After reperfusion, enzymology indicators such us creatine kinase (CK) and lactate dehydrogenase and the oxidative stress parameters such as nitric oxide (NO), malondialdehyde (MDA) and total superoxide dismutase (TSOD), and n-terminal pro-brain natriuretic peptide (NT-proBNP) expression was detected by immunohistochemistry. Results: LDH and CK levels of group A were significantly lower than the other three groups, and group B was the highest. There was significant difference between group B and group C (P<0.05), and no significant difference between group B and group D (P>0.05). MDA levels in group B were significantly higher than the other three groups. The lowest was group A, followed by group C, the difference among groups was significantly (P<0.05). TSOD and NO levels in group B was the lowest, the level in group A was the highest, followed by group C, the difference among groups was significant (P<0.05). NT-proBNP level in group B was significantly higher than the other three groups, the lowest was group A, followed by group C, the difference among groups was significant (P<0.05). Conclusions: Atorvastatin has a protective effect on the myocardial injury in the myocardial ischemia and reperfusion rats. It can increase NO synthesis and decrease MDA content, increase serum TSOD activity and the oxidative stress effect, meanwhile protect myocardial cells and reduce myocardial injury.
    14  Protective effect of omeprazole on gastric mucosal of cirrhotic portal hypertension rats
    Wei Gao Hai-Ying Li Li-Xin Wang Li-Jun Hao Jian-Li Gao Rong-Juan Zheng Chun-Jiang Cai Yan-Ling Si
    2014, 7(5):402-406. DOI: 10.1016/S1995-7645(14)60065-1
    [Abstract](30) [HTML](0) [PDF 445.63 K](194)
    Abstract:
    Objective: To observe the protective effect of omeprazole on gastric mucosal of cirrhotic portal hypertension rats. Methods: All rats were randomly divided into normal control group, cirrhosis and treatment group. Thioacetamide was used to establish rat model of cirrhotic portal hypertension. The necrotic tissue of gastric mucosa ulcer focus, degree of neutrophils infiltration at the ulcer margin, portal pressure, portal venous flow, abdominal aortic pressure, abdominal aortic blood flow at front end, gastric mucosal blood flow (GMBF), glycoprotein (GP) of gastric mucosa, basal acid secretion, H+back -diffusion, gastric mucosal damage index, NO, prostaglandin E2(PGE2) and tumor necrosis factor-α (TNF-α) were determined respectively, and the pathological changes of gastric mucosa were also observed by microscope. Results: Compared with cirrhosis group and the control group, the ulcer bottom necrotic material, gastric neutrophil infiltration and UI of the treatment group were all decreased significantly (P<0.01), GMBF value, GP values, serum NO, PGE2, TNF-α were all significantly increased. Conclusions: Omeprazole has an important protective effect on gastric mucosal and it can increase gastric mucosal blood flow and related to many factors.
    15  Effect of propofol and ketamine anesthesia on cognitive function and immune function in young rats
    Yan-Li Cao Wei Zhang Yan-Qun Ai Wen-Xia Zhang Yi Li
    2014, 7(5):407-411. DOI: 10.1016/S1995-7645(14)60066-3
    [Abstract](29) [HTML](0) [PDF 447.23 K](129)
    Abstract:
    Objective: To investigate the effects of propofol and ketamine on the cognitive function and immune function in young rats. Method: A total of 80 young rats were randomly divided into four groups: Control group, ketamine group (experimental group A), propofol group (experimental group B), ketamine and propofol group (experimental group C). All rats had continuous injection for three times, serum IL-2, IL-4 and IL-10 and whole brain IL-1β level, hippocampal neuronal apoptosis level were measured. The cognitive ability in rats was tested by water maze. Results: Water maze test showed on the 1st d, the maze test latency of the control group, the experimental group B and the experimental group C water were decreased gradually; Compared with the control group after 3 days, the latency of the experimental group A, experimental group B and experimental group C were all decreased, the crossing circle times were also reduced. Hippocampal neuron apoptosis were (2.3±1.7)%, (14.7±6.9)%, (4.2±3.3)%, (10.2±4.8)% in control group, experimental group A, experimental group B and experimental group C, respectively. The neurons apoptosis of experimental group A was significantly increased. The serum IL-4 and IL- 10 of the experimental group A, experimental group B and experimental group C after anesthesia were significantly higher than the control group. The whole brain IL-1β of the experimental group A, experimental group B and experimental group C were significantly lower than the control group. Conclusions: Propofol can reduce anesthesia effect of ketamine on the cognitive function and immune function in the young rats.
    16  Expression and distribution of TNF-α and PGE2 of periodontal tissues in rat periodontitis model
    Chu-Hang Liao Wei Fei Zhi-Hao Shen Ming-Ping Yin Chen Lu
    2014, 7(5):412-416. DOI: 10.1016/S1995-7645(14)60067-5
    [Abstract](37) [HTML](0) [PDF 428.63 K](228)
    Abstract:
    Objective: To simulate the expression of TNF-α andPGE2 of periodontal tissues in rat periodontitis model. Methods: 40 Wistar rats were randomly divided into the periodontitis group and the control group (n=20). After the successful establishment of periodontitis rat model, raising for six weeks before the animals were sacrificed. The periodontal tissues were obtained and made into slices. Observed the histopathological changes of the periodontal tissues and measured TNF-α,PGE2 levels change by immunohistochemistry, Western blot analysis and ELISA. Results: TNF-α,PGE2 expression of the periodontitis group was significantly higher than that in the control group, the difference was significant (P<0.05). Conclusions: The TNF-α,PGE2 expression of the rat periodontal tissue in the periodontitis group was significantly higher than the control group.
    17  Expression and function of CXCR2, CXCR7 of acute leukemic cells in rat
    Yuan-Lu Huang Xiao-Li Liu Na Xu Ya-Juan Xiao Guan-Lun Gao
    2014, 7(5):417-420. DOI: 10.1016/S1995-7645(14)60068-7
    [Abstract](31) [HTML](0) [PDF 418.94 K](144)
    Abstract:
    Objective: To investigate the expression and function of chemokine receptor CXCR2 and CXCR7 in the rat with acute leukemia. Methods: Flow cytometry and RT-PCR were used to detect the CXCR2, CXCR7 expression on the bone marrow cell surface of the acute leukemia group and the control group.Results: The bone marrow cell surface CXCR2, CXCR7 relative fluorescence intensity of the observation group was significantly higher than the control group (P<0.05). The CXCR7 expression of the extramedullary infiltration group was significantly higher than nonextramedullary infiltration group (P<0.05).The CXCR2, CXCR7mRNA median expression level of the observation group was higher than the control group. The CXCR2 expression and CXCR7 expression of the observation group was positively correlated, and the correlation coefficient was 0.782 (P<0.01). Conclusions: The chemokine receptor CXCR2 and CXCR7 are highly expressed in acute leukemia, which may be associated with the occurrence of leukemia

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