Objective: To study the value of serum procalcitonin content for assessing the inflammation and organ injury in neonatal sepsis. Methods: A total of 48 children with neonatal sepsis who were treated in our hospital between April 2014 and May 2016 were selected as the observation group, 50 healthy newborns who were delivered in our hospital during the same period were selected as the normal control group, and the observation group were further divided into high PCT group and low PCT group (n=24) according to the median of serum PCT content. Enzyme-linked immunosorbent assay (ELISA) was used to detect the serum contents of inflammatory mediators, and color Doppler diasonograph was used to measure heart injury index levels. Results: Peripheral blood PCT content of observation group was significantly higher than that of control group (P<0.05); serum inflammatory mediators IL-1β, IL-6, IL-8 and TNF-α contents of high PCT group and low PCT group were significantly higher than those of normal control group, and as the PCT content increased, serum inflammatory mediators IL-1β, IL-6, IL-8 and TNF-α contents increased (P<0.05); routine ultrasound parameters CO and LVEF levels as well as the absolute value of two-dimensional speckle tracking imaging parameters GLSr and GCSr of high PCT group and low PCT group were lower than those of normal control group while serum myocardial injury indexes cTnⅠ, H-FABP and HBDH contents were higher than those of normal control group, and as the PCT content increased, CO and LVEF levels as well as the absolute value of GLSr and GCSr decreased while the indexes cTn, H-FABP and HBDH contents increased (P<0.05). Conclusions: Serum PCT content is positively correlated with the degree of inflammation and heart injury in neonatal sepsis, and can be used as a reliability index to early judge the disease severity and the target organ damage.