[关键词]
[摘要]
目的:研究内异症不孕患者子宫内膜组织中mir-29c、mir-200a、mir-145表达量并分析下游分子。方法:选择在本院就诊的子宫内膜异位症所致不孕的女性患者作为研究的不孕组、同期因男方因素所致不孕的女性患者作为研究的对照组。收集子宫内膜组织并检测mir-29c、mir-200a、mir-145、HOXA-10、HOXA-11及下游分子、黏附分子的表达量。结果:不孕组患者子宫内膜组织组织中mir-29c、mir-200a、mir-145的表达量均显著高于对照组；不孕组患者子宫内膜组织中HOXA-10、HOXA-11、整合素αvβ3、IGFBP-1、CD44V6、N-cadherin、FAK的mRNA表达量均显著低于对照组且与mir-29c、mir-200a、mir-145的表达量呈负相关,E-cadherin、FUT4的mRNA表达量均显著高于对照组且与mir-29c、mir-200a、mir-145的表达量呈正相关。结论:内异症不孕患者子宫内膜组织中高表达的mir-29c、mir-200a、mir-145能够调节HOXA-10、HOXA-11及下游分子、黏附分子的表达并影响子宫内膜容受性。
[Key word]
[Abstract]
Objective: To study the expression of mir-29c, mir-200a and mir-145 in endometrial tissue and analyze the downstream molecules in infertile patients with endometriosis. Methods: Female patients with infertility caused by endometriosis who were treated in Leshan Maternal and Child Health Hospital between May 2014 and February 2017 were selected as the infertility group of the research, and female patients with infertility caused by male factors over the same period were selected as the control group of the research. Endometrial tissue was collected to detect the expression of mir-29c, mir-200a, mir-145, HOXA-10 and HOXA-11 as well as downstream molecules and adhesion molecules. Results: mir-29c, mir-200a and mir-145 expression in endometrial tissue of infertility group were significantly higher than those of control group; HOXA-10, HOXA-11, integrin αvβ3, IGFBP-1, CD44V6, N-cadherin and FAK mRNA expression in endometrial tissue of infertility group were significantly lower than those of control group and negatively correlated with mir-29c, mir-200a and mir-145 expression while E-cadherin and FUT4 mRNA expression were significantly higher than those of control group and positively correlated with mir-29c, mir-200a and mir-145 expression. Conclusion: The highly expressed mir-29c, mir-200a and mir-145 in endometrial tissue can regulate the expression of HOXA-10 and HOXA-11 as well as downstream molecules and adhesion molecules, and influence the endometrial receptivity in infertile patients with endometriosis.
[中图分类号]
R711.71
[基金项目]
四川省卫生厅科研课题(120390)
