Objective: To study the correlation of serum E-FABP content with glucose lipid metabolism, endothelial injury and inflammatory response in children with metabolic syndrome. Methods: 49 children with metabolic syndrome who were treated in the hospital between January 2016 and February 2017 were collected as observation group, and 50 healthy children who received vaccination in the hospital over the same period were collected as normal control group. Serum levels of E-FABP as well as glucose lipid metabolism, endothelial injury and inflammatory response indexes of two groups of subjects were detected, and the Pearson test was used to evaluate the correlation of E-FABP content with glucose lipid metabolism, endothelial injury and inflammatory response. Results:Serum E-FABP content of observation group was higher than that of control group; glucose metabolism indexes FBG and HOMA-IR levels in peripheral blood were higher than those of normal control group; lipid metabolism indexes TC, LDL-C and ApB contents in peripheral blood of observation group were higher than those of normal control group while HDL-C and ApA1 contents were lower than those of normal control group; endothelial injury index ET-1 content in serum was higher than that of normal control group while NO content was lower than that of normal control group; inflammatory response indexes CRP, IL-1β, IL-6 and IL-17 contents in serum were higher than those of normal control group (P<0.05). Pearson test showed that serum E-FABP content in children with metabolic syndrome was directly correlated with glucose lipid metabolism, endothelial injury and inflammatory response indexes. Conclusion: Serum E-FABP content increases abnormally in children with metabolic syndrome and is directly correlated with the abnormal degree of glucose lipid metabolism, endothelial injury and inflammatory response.