Objective: To investigate the effect of sufentanil on hemodynamics, oxidative stress and vascular endothelial function in patients undergoing laparoscopic cholecystectomy. Methods: A total of 88 patients undergoing laparoscopic cholecystectomy were divided into control group (n=44) and observation group (n=44) according to random data table, the patients in the control group received fentanyl anesthesia, and the observation group patients were given sufentanil anesthesia, the levels of hemodynamic, oxidative stress and vascular endothelial function at three times before anesthesia induction (T0), pneumoperitoneum (T1) and at the end of surgery (T2) between the two groups were compared. Results: At T0 moment, the levels of T0, SBP, HR, DBP, CAT, SOD, NO, MDA and ET-1 in the two groups were not statistically significant (P>0.05); Compared with the level of T0 moment, the levels of HR, SBP, DBP, and MDA in the two groups at T1 and T2 moment were increased in different degrees, and the levels of T1 and T2 in the observation group were significantly lower than those in the control group (P＜0.05); The levels of CAT and SOD in the two groups of T1 and T2 moment were lower than T0 moments in different degrees, and the observation group at two time levels were significantly higher than the control group (P＜0.05); The levels of NO and ET-1 in the two groups of T1 moment were significantly increased, and in the observation group the level of NO was significantly higher and ET-1 was significantly lower than that in the control group, all differences were statistically significant (P＜0.05); There was no significant difference of the NO and ET-1 levels at T2 between the two groups (P>0.05). Conclusion: Compared with fentanyl, sufentanil has less influence on hemodynamics in laparoscopic cholecystectomy. It can effectively reduce oxidative stress and protect vascular endothelial function, and is more conducive to recovery.