目的:探讨鹰嘴豆芽素A对宫颈癌细胞增殖及迁移能力的调控作用及其可能的分子机制。方法:体外培养Hela细胞,以不同质量浓度的鹰嘴豆芽素A处理宫颈癌Hela细胞,CCK-8法检测鹰嘴豆芽素A对宫颈癌细胞的增殖影响；划痕实验检测对细胞迁移的影响；RT-PCR法检测鹰嘴豆芽素A对Bcl-2 /Bax mRNA水平表达的变化。结果:鹰嘴豆芽素A能够抑制Hela细胞的增殖和迁移,呈现剂量和时间依赖性(P＜0.05)；质量浓度为10、20、40、80、160 μmol/L鹰嘴豆芽素A处理Hela细胞24 h、48 h后,抑制Hela细胞增殖和迁移速率明显上升,并且高于对照组(P＜0.05)；促凋亡蛋白Bax表达水平升高,抗凋亡蛋白Bcl-2表达水平降低,呈剂量依赖性。结论:鹰嘴豆芽素A能够抑制宫颈癌Hela细胞的增殖和迁移,其作用机制可能与下调Bcl-2,上调Bax有关。

Objective: The study is to investigate the regulation of Biochanin A on proliferation and migration of cervical cancer cells and its possible molecular mechanism. Methods: Hela cells were cultured in vitro and treated with different concentrations of Biochanin A. CCK-8 assay was used to detect the effect of Biochanin A on the proliferation of cervical cancer cells, while the Scratch assay was used to detect the effect on cell migration. Besides, the expression of Bcl-2 /Bax mRNA was detected by RT-PCR. Results: Biochanin A could inhibit the proliferation and migration of Hela cells in a dose-dependent and time-dependent manner. At 10,0,40,0,160 μmol/L of Biochanin A, the inhibition rate of Hela cells increased significantly after 24,8 h, significantly higher than that of the control group. The expression of pro-apoptotic protein Bax increased, while the expression of anti-apoptotic protein Bcl-2 decreased in a dose-dependent manner. Conclusion: Hummus A can inhibit the proliferation and migration of Hela cells in cervical cancer, and its mechanism may be related to the down-regulation of Bcl-2 and up-regulation of Bax.

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新乡医学院三全学院分子生物学与分子免疫学应用技术科技创新团队支持项目(STD201604)；新乡医学院三全学院骨干教师培育项目(SQ2019GGJS06)