目的:探讨卷曲蛋白10 (FZD10)在胃癌中的表达以及对胃癌细胞的影响。方法:运用免疫组化方法检测胃癌组织及癌旁组织中FZD10的表达；通过siRNA转染胃癌细胞系AGS和MGC-803,同时Western-blot检测转染效率,并运用CCK-8、划痕试验检测细胞增殖和侵袭能力。结果:FZD10在胃癌组织中的表达明显高于癌旁组织 (P＜0.05)；与空白对照组相比,siRNA转染胃癌细胞AGS和MGC-803后其增殖能力及细胞侵袭能力明显下降 (P<0.05),而空白对照组与空载阴性对照组之间无明显差异 (P＞0.05)。结论:在胃癌组织中,FZD10表达上调,沉默其表达后将影响胃癌细胞增殖和侵袭性能力。
Objective: To investigate the expressions of FZD10 and its effect on gastric cancer cells. Methods: The expression of FZD10 in gastric cancer tissues and adjacent cancer tissues was detected by immunohistochemistry；The gastric cancer cell lines AGS and MGC-803 were transfected with siRNA, and then use Western-blot to analysis the transfection efficiency; The proliferation and invasion ability of gastric cancer cell was analyzed by CCK-8 and wound heal. Results: The expression of FZD10 in gastric cancer was significantly higher than that in adjacent tissues (P<0.05). Compared with the blank control group, the proliferation and cell invasion ability of siRNA transfected with AGS and MGC-803 decreased significantly (P<0.05), while the blank control group and empty control group No significant difference between the negative control groups (P>0.05). Conclusion: The expressions of FZD10 significantly up-regulated in gastric cancer, and silencing its expression will affect the proliferation and invasive ability of gastric cancer cells.