Journal of Hainan Medical University(中文版)

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  • 1  重症肺炎患儿肠道菌群紊乱与全身炎症反应及应激反应程度的相关性
    陈曦 焦蓉 常立文
    2018, 24(13):1272-1275. DOI: 10.13210/j.cnki.jhmu.20180523.004
    [摘要](810) [HTML](0) [PDF 1.97 M](55)
    摘要:
    目的:研究重症肺炎患儿肠道菌群紊乱与全身炎症反应及应激反应程度的相关性。方法:选择2014年4月~2017年12月期间在襄阳市第一人民医院诊断为重症肺炎的患儿作为研究的肺炎组,另取同期在襄阳市第一人民医院体检的健康儿童作为对照组。留取粪便并测定肠道菌群双歧杆菌及大肠杆菌的数目,采集血清并测定炎症细胞因子及氧化应激指标的含量,采集外周血并测定炎症分子及氧化应激分子的表达强度。结果:肺炎组患儿粪便中双歧杆菌的数目以及双歧杆菌与大肠杆菌比值B/E的水平、血清中SOD的含量均显著低于对照组,粪便中大肠杆菌的数目、外周血中TLR2、TLR4、NOX2、iNOS、FOXP3的表达强度及血清中G-CSF、sTREM1、TNF-α、LPO、NO的含量显著高于对照组;Pearson相关性分析显示:肺炎组患儿粪便中B/E的水平与外周血中TLR2、TLR4、NOX2、iNOS、FOXP3的表达强度及血清中G-CSF、sTREM1、TNF-α、LPO、NO的含量呈负相关,与血清中SOD的含量呈正相关。结论:重症肺炎患儿肠道菌群的紊乱能够加重病程中全身炎症反应及应激反应的程度。
    2  阿托伐他汀联合曲美他嗪对冠心病患者氧化应激、血液流变学及NT-proBNP、hs-CRP的影响
    王丽岩 张洪磊 陈松
    2017, 23(17):2323-2327. DOI: 10.13210/j.cnki.jhmu.20170906.031
    [摘要](445) [HTML](0) [PDF 3.29 M](64)
    摘要:
    目的:探讨阿托伐他汀联合曲美他嗪对冠心病患者氧化应激、血液流变学及NT-proBNP、hs-CRP的影响。方法:选择84例于我院进行治疗的冠心病患者,随机分为观察组和对照组各42例。两组均接受冠心病的常规治疗,对照组加用阿托伐他汀治疗,观察组加用阿托伐他汀联合曲美他嗪治疗,两组均持续用药一个月。比较两组氧化应激指标:超氧化物歧化酶(SOD)、丙二醛(MDA),血液流变学指标:血沉、全血低切黏度、全血高切黏度、血浆粘度、纤维蛋白原(Fib)以及血浆N端脑钠肽前体(NT-proBNP)、高敏C反应蛋白(hs-CRP)指标的水平。结果:治疗前,观察组和对照组的氧化应激、血液流变学、NT-proBNP、hs-CRP指标之间比较差异无统计学意义(P>0.05)。与治疗前相比,治疗后观察组与对照组的SOD均显著升高,MDA均显著降低,差异均有统计学意义(P<0.05);治疗后,观察组的SOD高于对照组,观察组的MDA低于对照组,差异均有显著性(P<0.05)。与治疗前相比,治疗后观察组与对照组的血沉、全血黏度、血浆粘度和Fib等血液流变学指标均显著降低(P<0.05);治疗后,观察组的血沉、全血黏度、血浆粘度和Fib指标均显著低于对照组,各组差异均具有显著性(P<0.05)。相比于治疗前,治疗后两组NT-proBNP、hs-CRP水平均明显减少(P<0.05);治疗后观察组NT-proBNP、hs-CRP均明显低于对照组,差异有统计学意义(P<0.05)。结论:阿托伐他汀联合曲美他嗪治疗冠心病可明显降低氧化应激损伤、炎症反应及恢复正常的血液流变学水平,改善患者临床症状,值得推广。
    3  壮药南酸枣树皮对大鼠慢性难愈合创面PTEN、VEGF表达的影响
    何晓微 黄欣 狄钾骐 王兵 尹星
    2019(11):801-804, 809. DOI: 10.13210/j.cnki.jhmu.20190416.002
    [摘要](384) [HTML](0) [PDF 3.79 M](202)
    摘要:
    目的:利用壮药南酸枣树皮水煎剂干预慢性难愈合创面模型大鼠,探究其疗效机制。方法:采用全层皮肤缺损法改进慢性难愈合创面大鼠模型,将40只大鼠随机分为空白对照组、模型组、湿润烧伤膏组、南酸枣树皮组,空白对照组和模型组外敷生理盐水,湿润烧伤膏组采用皮肤再生医疗技术,南酸枣树皮组外敷南酸枣树皮水煎剂。12 d后取全层皮肤组织进行超微病理检测,利用RT-PCR技术观察PTEN、VEGF mRNA的变化情况。结果:经12 d药物干预后,湿润烧伤膏组、南酸枣树皮组皮肤细胞器的形态结构基本恢复。第12天湿润烧伤膏组、南酸枣树皮组分别与第1天相比,均出现PTEN mRNA水平降低、VEGF mRNA水平增高,差异具有统计学意义(P<0.05);第12天湿润烧伤膏组、南酸枣树皮组分别与模型组相比,均出现PTEN mRNA水平降低、VEGF mRNA水平增高,差异具有统计学意义(P<0.05);而湿润烧伤膏组与南酸枣树皮组相比差异无统计学意义 (P>0.05)。结论:壮药南酸枣树皮能显著改善慢性难愈合创面导致的细胞结构异常,调控PTEN介导的信号通路传导效应,改善损伤病灶,促进创面组织修复。
    4  DOS方案和SOX方案用于胃癌新辅助化疗的癌细胞杀伤效果比较
    陈亚妮 胡海峰
    2017, 23(13):1841-1843, 1847. DOI: 10.13210/j.cnki.jhmu.20170809.019
    [摘要](381) [HTML](0) [PDF 2.93 M](32)
    摘要:
    目的:研究DOS方案和SOX方案用于胃癌新辅助化疗的癌细胞杀伤效果。方法:选择在本院接受术前新辅助化疗的76例胃癌患者,随机分为接受奥沙利铂+替吉奥方案化疗的SOX组以及接受奥沙利铂+替吉奥+多西他赛方案化疗的DOS组。化疗前后测定血清肿瘤标志物的含量,化疗后测定手术切除病灶中增殖基因、抑癌基因、侵袭基因的表达量。结果:两组化疗后血清中CA72-4、G17、TK-1的含量均显著低于化疗前且DOS组化疗后血清中CA72-4、G17、TK-1的含量均显著低于SOX组;化疗后,DOS患者手术切除病灶中CyclinB、CyclinD1、CDK1、CDK4、CDK6、Vav2、MMP2、ADAM8、ITF2的mRNA表达量显著低于SOX组,RASSF1A、Noxa、GKN1、p16ink4a的mRNA表达量显著高于SOX组。结论:DOS新辅助化疗方案能够较SOX方案更为有效地杀伤胃癌细胞并抑制癌细胞增殖、侵袭。
    5  Research of the anxiolytic effect of Baihe Dihuang Decoction
    Fang Huan-Le Han Ning-Juan Li Xiao-Ming Niu Rui Chen Yan-Bin
    2019, 25(5):6-9.
    [摘要](318) [HTML](0) [PDF 0.00 Byte](462)
    摘要:
    Objective: To study the effect of Baihe Dihuang Decoction in improving anxiety and its mechanism of action. Methods: Male mice were randomly divided into 5 groups: blank control group, Baihe Dihuang Decoction high, medium, low-dose group and diazepam group. After continuous intragastric administration for 14 d, the behavioral test of the mouse elevated plus maze experiment and opening test was performed. The brain tissue GABA and Glu content was measured by immunosorbent assay (ELISA). Results: Compared with the blank control group, the middle and high doses of Baihe Dihuang Decoction could increase the time and frequency of movement of the mice in the open cross labyrinth in the open arm and increase the number of times that the mouse entered the central area in the opening test. The anti-anxiety effect also showed an increase in the brain tissue GABA content in mice, ignificantly decreased Glu contents in mice. Conclusion: Baihe Dihuang Decoction has some anxiolytic effect, and antianxiety effect may be related to increasing brain tissue GABA content, decreased Glu contents in mice.
    6  舒筋洗外用颗粒治疗指屈肌腱狭窄性腱鞘炎疗效观察
    苏畅 王应琼
    2019(11):845-848. DOI: 10.13210/j.cnki.jhmu.20190430.001
    [摘要](304) [HTML](0) [PDF 2.22 M](196)
    摘要:
    目的:观察舒筋洗外用颗粒熏洗在指屈肌腱狭窄性腱鞘炎的疗效。方法:将240例指屈肌腱狭窄性腱鞘炎患者按就诊先后顺序随机分为温水组、颗粒组、中药组各80例,温水组采用温开水熏洗配合扶他林软膏治疗,颗粒组采用舒筋洗外用颗粒熏洗配合扶他林软膏治疗,中药组采用中药煎剂熏洗配合扶他林软膏治疗。观察记录3组患者治疗前及治疗8周后视觉疼痛模拟评分(VAS),在治疗8周后记录其临床疗效。结果:治疗8周后,颗粒组、中药组VAS评分明显低于温水组(P<0.05);颗粒组、中药组临床疗效明显优于温水组(P<0.05);而颗粒组与中药组的VAS评分、临床疗效比较,差异无统计学意义(P>0.05)。结论:舒筋洗外用颗粒熏洗在指屈肌腱狭窄性腱鞘炎的治疗中疗效确切,值得临床推广、应用。
    7  Promotional effects of exogenous stimulation with pulsedelectromagnetic fields on skin wound healing in diabetic rats
    Yan Han Ze-Dong Yan Shu-Hua Ge
    2019, 25(5):1-5.
    [摘要](296) [HTML](0) [PDF 0.00 Byte](422)
    摘要:
    Objective: The current study aims to identify the effects of exogenous application of pulsed electromagnetic fields on skin wound healing in diabetic rats, and thus provides experimental evidence for its more scientific clinical application in the future. Methods: The diabetic animal models were established via intraperitoneal injection of streptozotocin in 4-monthold male rats. Fourth eight rats were randomly assigned into the diabetes group (DM) and diabetes coupled with pulsed electromagnetic fields (DM+PEMF) group. Another 24 normal rats were used as the blank control group (Control). Then, all the rats in the three groups were subjected to dorsal surgery for the establishment of soft tissue wound model (circular wound with the diameter of 2 cm). Then, the rats in the DM+PEMF group were subjected to pulsed electromagnetic fields stimulation. Then, 6 rats in each group were sacrificed at Day 5, 12 and 19 post surgery. The glucose levels, wound closure, wound healing time and tissue tensile strength were examined and analyzed. Results: Pulsed electromagnetic fields significantly increased the wound closure rate in diabetic rats at Day 5, 12 and 19 post surgery, decreased overall wound healing period in diabetic rats, and also enhanced tissue tensile strength in diabetic rats at Day 5, 12 and 19 post surgery. Conclusion: Low-intensity pulsed electromagnetic fields can significantly accelerate diabetic wound healing process, and also improve diabetic tissue repair capacity. This study may be helpful for providing more scientific and reasonable experimental evidence for the treatment of pulsed electromagnetic fields on diabetic wound healing in clinics.
    8  Graves病患者外周血NKT细胞数目、功能的评估及其与甲亢病情的相关性研究
    李兰 杨丽 袁丽 叶晓贤 游敏
    2017, 23(11):1485-1487, 1491. DOI: 10.13210/j.cnki.jhmu.20170525.016
    [摘要](295) [HTML](0) [PDF 2.88 M](49)
    摘要:
    目的:研究Graves病患者外周血NKT细胞数目、功能的评估及其与甲亢病情的相关性。方法:选择在我院确诊为Graves病的患者作为GD组,同期在我院健康体检的55例健康志愿者作为对照组。采集外周血并测定CD3+CD56+NKT细胞的数目,采集血清并检测细胞因子及甲状腺功能指标的含量。结果:Graves组患者外周血中CD3+CD56+NKT细胞的数目显著低于对照组;GD组患者血清中IL-2、IFN-γ、TNF-α、IL-10、TGF-β的含量显著低于对照组且与外周血中CD3+CD56+NKT细胞的数目呈正相关,IL-4、IL-5、IL-17、FT3、FT4、TPOAb、TgAb、TRAb的含量显著高于对照组且与外周血中CD3+CD56+NKT细胞的数目呈负相关。结论:Graves病患者外周血NKT细胞数目的异常减少能够影响Th1/Th2、Th17/Treg的平衡并增加TRAb的分泌,进而引起甲状腺功能亢进。
    9  伊伐布雷定对心肌梗死大鼠心肌细胞Notch和 NF-κB信号通路的影响
    吴婷玉 吴嬛 陈诚
    2019(11):810-814. DOI: 10.13210/j.cnki.jhmu.20190404.003
    [摘要](289) [HTML](0) [PDF 2.81 M](205)
    摘要:
    目的:探讨伊伐布雷定(IVA)对心肌梗死大鼠心肌细胞Notch和 NF-κB信号通路的影响。方法:采用结扎冠状动脉左前降支建立心肌梗死模型,将存活大鼠随机分为模型组(MI组,n=8)和治疗组(IVA组,n=8)。以相同部位穿线但不结扎冠状动脉左前降支的大鼠作为对照组(CON组,n=8)。 IVA给药28 d。检测所有大鼠血流动力学和心功能指标:心率(HR)、收缩压(SBP)、舒张压(DBP)、平均动脉压(MAP)、左室收缩压(LVSP)、左室舒张末压(LVEDP)和左室内压最大上升和下降速率(±dp/dt);左室重量指数、左室截面直径和心肌梗死面积;RT-PCR检测大鼠心肌细胞Notch信号通路成分mRNA的表达水平(Notch-1、Dll-4、Hes-1);Western-blot检测DICD-1蛋白和P65蛋白表达水平。组间比较采用单因素方差分析,两两比较采用 SNK法。结果:MI组SBP、 DBP、MAP、LASP、LVEDP和±dp/dt均低于对照组(P<0.05);而IVA上述指标均高于MI组(P<0.05)。MI组左室重量指数和左室截面直径明显大于对照组(P<0.05),但小于IVA组(P<0.05)。MI组Notch-1 mRNA表达水平明显高于对照组(P<0.05),但低于IVA组(P<0.05)。3组Dll-4和Hes-1 mRNA表达水平的比较差异无统计学意义(P>0.05)。MI组心肌细胞NICD-1蛋白和P65蛋白表达水平明显高于CON组(P<0.05),但是低于IVA组(P<0.05)。结论:IVA可能通过Notch和 NF-κB信号通路发挥改善心肌梗死大鼠心功能和抑制心室重构的作用。
    10  不同肿瘤标志物联合检测对原发性肝癌的诊断效能分析
    高鹏飞 杨豪俊
    2019(11):858-861, 866. DOI: 10.13210/j.cnki.jhmu.20190401.007
    [摘要](283) [HTML](0) [PDF 3.01 M](196)
    摘要:
    目的:探索不同肿瘤标志物联合检测对原发性肝癌的诊断效能,为临床诊断提供参考依据。方法:收集来我院就诊的临床诊断为原发性肝癌的患者72例作为观察组,诊断为良性肝病的患者65例作为良性肝病组,80例健康体检者作为健康对照组,所有对象采用化学发光法测定肿瘤标志物甲胎蛋白(AFP) 、癌胚抗原(CEA)、糖类抗原-199(CA199)、糖类抗原-125(CA125)和糖类抗原-153(CA153)的含量,然后对单组患者的5种肿瘤标志物的检测结果、各指标对各组检测阳性率进行比较,并对不同肿瘤标志物单独检测与联合检测原发性肝癌的诊断价值分析。结果:观察组AFP、CA199 和CA153的值高于良性肝病组,AFP 、CEA、 CA199、 CA125 和CA153的值高于健康对照组,良性肝病组的CA199 和CA125的值高于健康对照组,差异具有统计学意义(P<0.05);AFP 、CEA、 CA199 和CA153对观察组的检测阳性率高于良性肝病组,AFP 、CEA、 CA199和CA125 对观察组的检测阳性率高于健康对照组,AFP 、CEA、 CA199和CA125 对良性肝病组的检测阳性率高于健康对照组,差异具有统计学意义(P<0.05);各指标联合检测原发性肝癌的灵敏度为86.4%,特异度为89.2%,正确指数为75.6%,误诊率为13.6%,漏诊率为10.8%,且联合检测比各指标单独检测的正确指数都高。结论:血清肿瘤标志物AFP 、CEA、 CA199、 CA125 和CA153联合检测可提高原发性肝癌诊断的灵敏度和特异度,对原发性肝癌有较好的诊断价值。
    11  绝经后女性Prdxs蛋白家族水平与骨质疏松的关系
    方虹 鲁林
    2019(11):839-844. DOI: 10.13210/j.cnki.jhmu.20190401.003
    [摘要](268) [HTML](0) [PDF 3.42 M](201)
    摘要:
    目的:观察过氧化物还原酶(Prdxs)蛋白家族主要分子Prdx1、Prdx2、Prdx4和Prdx6在女性绝经后骨质疏松症(PMOP)中的表达水平,并分析其临床诊断价值。方法:选择湖北省中医院及武汉市中医院确诊的PMOP患者72例作为研究组,另选择同期骨密度正常的绝经后女性51例作为对照组。免疫酶联吸附实验(ELISA)测定血浆Prdx1、Prdx2、Prdx4和Prdx6的蛋白水平;逆转录定量聚合酶链反应(RT-qPCR)测定外周血单个核细胞(PBMC)中Prdx1、Prdx2、Prdx4和Prdx6的mRNA水平。分析Prdxs家族与患者临床参数的相关性。受试者工作特征曲线(ROC)评价Prdxs家族对女性PMOP的诊断价值。结果:研究组的血浆Prdx1、Prdx4和Prdx6水平显著低于对照组(P<0.05)。研究组PBMC中Prdx1和Prdx6的mRNA相对表达水平显著低于对照组(P<0.05)。研究组血浆及PBMC中多种Prdxs的蛋白水平或mRNA水平与血脂或炎症指标相关(P<0.05)。血浆Prdx6诊断PMOP的曲线下面积(AUC)为0.794(95%CI =0.714~0.874),PBMC中Prdx6的mRNA相对表达水平诊断PMOP的AUC为0.725(95%CI =0.635~0.814)。结论:Prdxs蛋白家族的表达降低(特别是Prdx1和Prdx6分子)与PMOP的发病密切相关,降低的Prdxs蛋白家族可能是通过脂代谢异常途径和系统性炎症水平增高途径来促进骨质疏松的发生。检测血浆和PBMC中的Prdx6分子水平对PMOP具有着良好的诊断价值。
    12  注意缺陷多动障碍模型大鼠HPA轴功能的变化研究
    唐彦 景晓玉 王渝评 徐寅
    2019(11):815-817, 825. DOI: 10.13210/j.cnki.jhmu.20190409.003
    [摘要](263) [HTML](0) [PDF 2.52 M](209)
    摘要:
    目的:观察注意缺陷多动障碍(ADHD)模型动物自发性高血压大鼠(SHR)血清皮质酮(CORT)、促肾上腺皮质激素(ACTH)、促肾上腺皮质激素释放激素(CRH)水平及下丘脑糖皮质激素受体(GR)、糖皮质激素受体(MR)、CRH及海马GR、MR mRNA的表达情况,探讨SHR大鼠下丘脑-垂体-肾上腺轴(HPA)功能及相关影响因素。方法:以幼年SHR大鼠为ADHD模型组、Wistar京都大鼠(WKY)为对照组,采用ELISA法检测血清CORT、ACTH、CRH水平,RT-PCR法检测大鼠下丘脑GR、MR、CRH及海马GR、MR mRNA的表达水平。结果:SHR大鼠血清CORT、ACTH水平明显低于WKY大鼠(P<0.01),血清CRH水平明显高于WKY大鼠(P<0.01),下丘脑及海马GR、MR mRNA的表达水平明显高于WKY大鼠(P<0.01),而下丘脑CRH mRNA表达则低于WKY大鼠(P<0.01)。结论:SHR大鼠HPA轴呈低反应性,可能与CRH启动障碍及下丘脑和海马GR、MR mRNA 高表达相关。
    13  兔蛛网膜下腔出血应用尼莫地平治疗的生物利用度研究
    杨光 李香营 王雄 陈晶
    2019(11):826-828, 833. DOI: 10.13210/j.cnki.jhmu.20190426.006
    [摘要](262) [HTML](0) [PDF 2.51 M](197)
    摘要:
    目的:观察皮下注射尼莫地平和口服尼莫地平在健康兔和蛛网膜下腔充血(SAH)兔体内的生物利用度,并探讨皮下给药是否优于口服给药并达到目标血清浓度。方法:采用随机数字表法将成年雄性NZW兔36只分为6组(n=6):口服尼莫地平组(5、15 mg/kg组),皮下注射尼莫地平组(2.5、5、15 mg/kg组),SAH+皮下注射尼莫地平组(2.5 mg/kg),测量各组尼莫地平的血浆浓度并进行统计学分析。结果:(1)口服15 mg/kg的尼莫地平会产生更高的峰值血浆浓度(Tmax),并且增加峰值血浆浓度(Cmax),曲线下面积(AUC)亦大于5 mg/kg。对于皮下给药后观察到达到Tmax的时间具有相反的趋势,其中15 mg/kg导致较低的Tmax,并且具有比5 mg/kg更低的AUC倾向。(2)相比口服给药,皮下注射组的平均尼莫地平浓度显著大于7 ng/mL(P <0.01),且尼莫地平血浆浓度保持在7 ng/ml以上的时间明显大于口服给药(P<0.01)。(3)健康NZW兔中2.5 mg/kg皮下尼莫地平产生的Cmax、Tmax和AUC值,与5和15 mg/kg皮下注射后测量值无显著性差异(P>0.05)。在24 h测量的平均尼莫地平浓度高于7 ng/mL的目标治疗水平条件下,健康NZW兔(12.9±10.0)ng/mL和SAH(11.8±4.6)ng/mL兔差异亦无统计学意义(P> 0.05)。结论:皮下施用尼莫地平优于口服尼莫地平,并可以将24 h后尼莫地平血浆水平维持在7 ng/mL以上,有助于研究尼莫地平治疗SAH后延迟血管痉挛的机制。
    14  Effect of shexiangbaoxin pill combined with tirofiban hydrochloride on related factors in elderly patients with acute coronary symdrome
    Ya-Ning Xu Ling-Zhi Tang Xiao-Li Sun Chao Ruan Yue Wu Li-Li Li
    2019, 25(5):37-40.
    [摘要](259) [HTML](0) [PDF 0.00 Byte](211)
    摘要:
    Objective: To study the effect of shexiangbaoxin pill combined with tirofiban hydrochloride on related factors in elderly patients with acute coronary symdrome. Methods: A total of 96 elderly patients with acute coronary symdrome in our hospital from January 2015 to May 2018 were enrolled in this study. The subjects were divided into the control group (n=48) and the treatment group (n=48) randomly. The control group were treated with tirofiban hydrochloride, the treatment group were treated with shexiangbaoxin pill combined with tirofiban hydrochloride, and both the two groups were treated for 2 weeks. The PT, TT, APTT and serum FIB, CK-MB, cTnT, vWF, ET-1, NO, sICAM-1, MMP-9, hs-CRP of the two groups before and after treatment were compared. Results: There were no significantly differences of the PT, TT, APTT and serum FIB, CK-MB, cTnT, vWF, ET-1, NO, sICAM-1, MMP-9, hs-CRP of the two groups before treatment. The PT, TT, APTT and serum NO of the two groups after treatment were significantly higher than before treatment, the serum FIB, vWF, ET, sICAM-1, MMP-9, hs-CRP of the two groups after treatment were significantly lower than before treatment, and that of the treatment group after treatment were significantly better than the control group. The serum CK-MB, cTnT of the two groups after treatment were significantly higher than before treatment, but that of the treatment group after treatment were significantly lower than the control group. Conclusion: Shexiangbaoxin pill combined with tirofiban hydrochloride can significantly improve the blood coagulation function and reduce the Myocardial injury, vascular endothelial damage, inflammation of the elderly patients with acute coronary symdrome, and it was worthy clinical application.
    15  鹰嘴豆芽素A对Hela细胞增殖、迁移及Bcl-2、Bax基因表达的影响
    赵凤婷 王朋博 王冰 张宁 薛钊 孙志彬 原文政 姬国杰 丰慧根 胡焕焕
    2019(11):805-809. DOI: 10.13210/j.cnki.jhmu.20190404.002
    [摘要](253) [HTML](0) [PDF 3.18 M](213)
    摘要:
    目的:探讨鹰嘴豆芽素A对宫颈癌细胞增殖及迁移能力的调控作用及其可能的分子机制。方法:体外培养Hela细胞,以不同质量浓度的鹰嘴豆芽素A处理宫颈癌Hela细胞,CCK-8法检测鹰嘴豆芽素A对宫颈癌细胞的增殖影响;划痕实验检测对细胞迁移的影响;RT-PCR法检测鹰嘴豆芽素A对Bcl-2 /Bax mRNA水平表达的变化。结果:鹰嘴豆芽素A能够抑制Hela细胞的增殖和迁移,呈现剂量和时间依赖性(P<0.05);质量浓度为10、20、40、80、160 μmol/L鹰嘴豆芽素A处理Hela细胞24 h、48 h后,抑制Hela细胞增殖和迁移速率明显上升,并且高于对照组(P<0.05);促凋亡蛋白Bax表达水平升高,抗凋亡蛋白Bcl-2表达水平降低,呈剂量依赖性。结论:鹰嘴豆芽素A能够抑制宫颈癌Hela细胞的增殖和迁移,其作用机制可能与下调Bcl-2,上调Bax有关。
    16  Effects of selenium supplementation on thyroid function, immune response and oxidative stress in HT patients
    Chun-Mei Qu? Ying Li Shu-Mei Chen
    2019, 25(5):45-48.
    [摘要](248) [HTML](0) [PDF 0.00 Byte](212)
    摘要:
    Objective: To explore the effect of selenium supplementation on thyroid function, immune response and oxidative stress in HT patients with normal thyroid function. Methods: A total of 120 Hashimoto's thyroiditis patients with normal thyroid function admitted to our hospital from June 2016 to March 2018 were randomly divided into treatment group and control group with 60 cases in each group. The control group was given oral placebo, while the treatment group was given selenium yeast. The expression levels of thyroid function [including thyroid stimulating hormone (TSH), free triiodothyronine (FT3) and free thyroxine (FT4)], immune response [including thyroid peroxidase antibody (TPOAb), thyroglobulin antibody (TGAb), interferon-γ (IFN-γ), interleukin-4 (IL-4)] and oxidative stress [including malondialdehyde (MDA), glutathione peroxidase (GPx) and superoxide dismutase (SOD)] were observed and compared between the two groups. Results: The two groups showed significant changes in auxiliary diagnosis, immune response and oxidative stress. Before treatment, there was no significant difference in the levels between the two groups. After treatment, there was no significant difference in the above indexes between the control group and that before treatment. There was no significant difference in thyroid function related indexes between the treatment group and the control group. The levels of TPOAb, TGAb, IFN-γ and MDA in the treatment group were significantly lower than those before treatment and in the control group, while the levels of IL-4, GPx and SOD were significantly higher than those before treatment and in the control group. Conclusions: Selenium supplementation can stabilize thyroid function, reduce the level of thyroid antibody production, and improve immune response and oxidative stress response in HT patients.
    17  泼尼松、环磷酰胺联合川芎嗪注射液对系统性红斑狼疮患者免疫功能及其他相关因子水平的影响
    闾志敏 顾菁 叶丽萍
    2019(11):834-838. DOI: 10.13210/j.cnki.jhmu.20190401.001
    [摘要](247) [HTML](0) [PDF 2.64 M](193)
    摘要:
    目的:探讨在泼尼松和环磷酰胺的基础上加用川芎嗪注射液对系统性红斑狼疮(SLE)患者免疫功能及其他相关因子水平的影响。方法:选取我院诊治的70例SLE患者,随机分为对照组和观察组各35例。对照组应用泼尼松联合环磷酰胺进行治疗,观察组在对照组用药的基础上加用川芎嗪注射液静脉滴注。于治疗前后检测并比较两组免疫指标(IgG、C3、ANA)、基质金属蛋白酶(MMP-3、MMP-9、TIMP1)、趋化因子(CXCL9,CXCL10、CXCL11)以及血清IL-10、PRL、S100p、EET水平。结果:治疗前,两组免疫指标(IgG、C3、ANA)、基质金属蛋白酶(MMP-3、MMP-9、TIMP1)、趋化因子(CXCL9,CXCL10、CXCL11)以及血清IL-10、PRL、S100p、EET水平比较,差异无统计学意义(P>0.05);治疗后,两组免疫指标(IgG、C3、ANA)、基质金属蛋白酶(MMP-3、MMP-9)、趋化因子(CXCL9,CXCL10、CXCL11)以及血清IL-10、PRL、S100p、EET水平比较,差异具有统计学意义(P<0.05)。结论:对系统性红斑狼疮患者在泼尼松和环磷酰胺联合治疗的基础上加用川芎嗪注射液,不仅能够改善患者的免疫功能指标,而且能够改善基质金属蛋白酶、趋化因子以及相关血清因子水平,值得临床上研究应用。
    18  卵巢癌患者血清中ROBO4、sB7-H4含量与肿瘤病理特征的相关性研究
    于文亮
    2019(11):877-880. DOI: 10.13210/j.cnki.jhmu.20190507.003
    [摘要](241) [HTML](0) [PDF 2.22 M](198)
    摘要:
    目的:研究卵巢癌患者血清中ROBO4、可溶性B7-H4(sB7-H4)含量与肿瘤病理特征的相关性。方法:选择本院手术切除治疗的卵巢癌患者及卵巢良性肿瘤患者,测定血清中ROBO4、sB7-H4的含量以及病灶组织中ROBO4、B7-H4、癌基因的表达量。结果:卵巢癌患者血清中ROBO4的含量较卵巢良性肿瘤患者显著降低,sB7-H4的含量较卵巢良性肿瘤患者显著升高;卵巢癌组织中ROBO4的mRNA表达量较卵巢良性肿瘤组织显著降低,B7-H4的mRNA表达量较卵巢良性肿瘤组织显著升高,且卵巢癌患者血清中ROBO4、sB7-H4的含量与卵巢癌组织中ROBO4、B7-H4的mRNA表达量具有正相关关系;肿瘤的FIGO分期及病理分级越高,卵巢癌患者血清中ROBO4的含量越低、sB7-H4的含量越高;卵巢癌组织中β-catenin、CyclinD1、MMP9的mRNA表达量与卵巢良性肿瘤组织比较显著升高且与ROBO4呈负相关、与sB7-H4呈正相关,PTEN、E-cadherin的mRNA表达量与卵巢良性肿瘤组织比较显著降低且与ROBO4呈正相关、与sB7-H4呈负相关。结论:卵巢癌患者血清中ROBO4、sB7-H4含量的变化与肿瘤临床病理分期改变、癌基因异常表达均密切相关。
    19  宫瘤消胶囊对子宫肌瘤患者性激素、肿瘤标志物、炎症因子及血管内皮功能的影响
    秦传芬 李孝军 张宏玲
    2019(11):862-866. DOI: 10.13210/j.cnki.jhmu.20190330.002
    [摘要](239) [HTML](0) [PDF 2.81 M](200)
    摘要:
    目的:探讨宫瘤消胶囊对子宫肌瘤患者炎症因子、肿瘤标志物、性激素及血管内皮功能的影响。方法:选取我院妇科收治的子宫肌瘤患者130例,依据治疗方式分为单一治疗组和联合用药组,每组各65例。联合治疗组采用米非司酮联合宫瘤消胶囊治疗,单一治疗组采用米非司酮口服治疗。比较两组治疗前后炎症因子、肿瘤标志物、性激素及血管内皮功能的变化。结果:两组在治疗前性激素、炎症因子、肿瘤标志物及血管内皮功能指标水平比较, 差异无统计学意义(P>0.05)。两组治疗后性激素雌二醇(E2)、孕酮(P)、卵泡生成激素(FSH)和黄体生成激素(LH)水平,肿瘤标志物癌抗原125(CA125)及糖蛋白抗原19-9(CA19-9)水平,炎症因子肿瘤坏死因子-α(TNF-α)、金属蛋白酶-9(MMP-9)及胰岛素样生长因子1(IGF-1)水平及血管内皮功能血管内皮生长因子(VEGF)及碱性成纤维生长因子(bFGF)水平均显著降低(P<0.05)。联合用药组E2、P、FSH及LH均明显低于单一用药组水平(P<0.05)。联合用药组CA125及CA19-9均明显低于单一用药组水平(P<0.05)。联合用药组TNF-α、MMP-9及IGF-1均明显低于单一用药组水平(P<0.05)。联合用药组VEGF及b-FGF均明显低于单一用药组水平(P<0.05)。结论:米非司酮联合宫瘤消胶囊治疗可缓解子宫肌瘤患者改善内分泌紊乱,缓解炎症应激反应,改善血管内皮功能,值得临床推广应用。
    20  Development and prospects of cervical laminoplasty
    Wen-Xuan Wang Bin Zhao Xiao-Feng Zhao Shao-Wei Wang
    2019, 25(5):73-76.
    [摘要](239) [HTML](0) [PDF 0.00 Byte](300)
    摘要:
    Cervical laminoplasty surgery is an alternative to laminectomy. It can increase the space of the spinal cord available by raising the lamina, and achieve the indirect decompression effect to treat cervical spondylosis. Various techniques of laminoplasty have since been developed after two prototype techniques: Hirabayashi’s open-door laminoplasty and Kurokawa’s double-door laminoplasty. Several studies report superior biomechanical stability of the cervical spine after laminoplasty compared with laminectomy. Recent development in laminoplasty is preservation of muscle attachment, which enabled dynamic and static stabilization of the cervical spine by neck extensor muscles. After treatment with new laminoplasty techniques with active postoperative neck muscle exercises, postoperative instability, kyphosis, axial neck pain, and loss of ROM become minimal. The development,complications and future trends of cervical laminoplasty are summarized below.

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