Journal of Hainan Medical University(中文版)
    全选反选导出
    显示模式:
    2022,28(22):1681-1687, DOI: 10.13210/j.cnki.jhmu.20220617.001
    摘要:
    目的:通过GC⁃MS代谢组学方法,研究了补阳还五汤对D⁃gal致衰老模型小鼠学习记忆能力的保护效果。方法:选择了24只3月龄的昆明动物作为实验样本,按体重大小随机分成空白组、模型组、补阳还五汤组,通过新体识别试验检测实验动物的记忆水平,HE染色检测动物神经元结构等。采用气相色谱⁃质谱联用仪对各组实验动物血浆进行了全面定量分析,并利用血浆的代谢过程追溯了病变发生进程中的重要代谢物和主要代谢途径。结果:在HE染色中,与空白组比较,模型组神经元排列异常,形态改变;与模型组比较,补阳还五汤组神经元排列规则,形态正常。在新物体识别实验中,模型组小鼠新物体识别指数降低;补阳还五汤组小鼠新物体识别指数升高。通过GC⁃MS技术,发现了5种AD的血浆差异代谢产物,分别是L⁃焦谷氨酸、赖氨酸、焦磷酸、肌酸酐、α⁃乳糖。结论:补阳正五汤对D⁃gal模型小鼠疗效显著,并能有效提高其学习记忆的水平。其作用机制可能与谷胱甘肽代谢以及氨酰生物合成等相关代谢通路有关。
    2022,28(22):1688-1693, DOI: 10.13210/j.cnki.jhmu.20220705.003
    摘要:
    目的:研究益肾祛痛颗粒对骨癌痛模型大鼠痛觉敏化及骨破坏的影响。方法:Walker256细胞经腹水传代后注射于雌性Wistar大鼠胫骨制备骨癌痛模型,60只大鼠随机分成假手术组、模型组及益肾祛痛颗粒低剂量组、中剂量组、高剂量组和盐酸曲马多阳性药组,造模后第14天给药,连续给药14 d,观察各组大鼠痛阈值及后肢负重差。结果:与模型组比较,益肾祛痛颗粒各组均可提高大鼠痛阈值,减小双后肢负重差(P<0.05),与阳性药组比,益肾祛痛颗粒中剂量组在提高大鼠机械性痛阈值、热痛阈值方面无统计学差异(P>0.05),益肾祛痛颗粒各组大鼠后肢负重差值均小于阳性药组(P<0.05)。结论:益肾祛痛颗粒可以缓解骨癌痛大鼠机械性痛觉敏化,并能减轻骨癌痛大鼠的骨质破坏。
    2022,28(22):1694-1703, DOI: 10.13210/j.cnki.jhmu.20220908.003
    摘要:
    目的:观察不稳定型心绞痛(UA)与急性心肌梗死(AMI)患者的血浆代谢组分,筛选潜在的差异性标志物,探讨可能的生物学机制,为急性冠脉综合征的早期评估提供参考。方法:收集UA和AMI患者的血浆样本,获取一般信息,运用液质联用技术检测代谢物。通过偏最小二乘判别分析、正交化偏最小二乘判别分析模型,并结合单变量统计分析,进一步确定显著差异代谢物及其通路。结果:共纳入UA组33例、AMI组47例样本进行检测,发现UA与AMI的显著差异代谢物54种,代谢通路共20条。泮托拉唑、乙酰左旋肉碱、棕榈酰乙醇酰胺、甜菜碱、辛酸、胆红素、组氨酸、油酸、枸橼酸盐、香草醛等代谢物可作为鉴别二者的潜在生物标志物,其涉及的通路包括ABC转运蛋白、氨酰⁃tRNA生物合成、癌症的中心碳代谢等。结论:UA与AMI的血浆代谢组分存在显著差异,代谢组学相关研究对探求其生物学本质具有一定指导意义。
    2022,28(22):1704-1709, DOI: 10.13210/j.cnki.jhmu.20220908.001
    摘要:
    目的:通过检测子痫前期(PE)及正常孕妇胎盘中程序性细胞死亡受体1(programmed cell death 1,PD‑1)及其配体PD‑L1、调节性细胞和辅助性T细胞的特异性核转录因子叉头状螺旋转录因子3(forkhead box protein 3,Foxp3)和维甲酸相关孤核受体γc (retinoci acid receptor‑related orphan receptoe gammat,RORγc)的表达水平,研究它们的差异表达与PE发病的关系。方法:选取2021年3月~2022年3月在海南医学院附属海南医院产科接受诊治的子痫前期患者40例,分为轻度PE组(PE组,n=20)和重度PE组(sPE组,n=20),另取同期剖宫产终止妊娠的正常孕妇作为对照组(对照组,n=20)。收集3组孕妇剖宫产后的胎盘组织,采用实时荧光定量PCR检测胎盘PD‑1、PD‑L1、Foxp3和RORγc的mRNA表达,而蛋白表达情况采用蛋白质印迹法检测,并分析其与PE发生发展的关系。结果:(1)qRT‑PCR法检测结果表明,与对照组相比,PE组和sPE组的PD‑1、PD‑L1、Foxp3的mRNA表达均显著下降;与PE组相比,sPE组患者3项指标的mRNA表达水平下降的更显著,3组数据两两比较差异均有统计学意义(P<0.05)。与对照组相比,PE组和sPE组RORγc的mRNA表达均显著上升;与PE组相比,sPE组患者RORγc的mRNA表达水平升高更显著,3组数据两两比较差异均有统计学意义(P<0.05)。(2)蛋白质印迹法结果显示,与对照组相比,PE组和sPE组的PD‑1、PD‑L1、Foxp3 蛋白表达水平明显下降;与PE组相比,sPE组患者3项指标的蛋白表达水平下降更显著,3组数据两两比较差异均有统计学意义(P<0.05)。与对照组相比,PE组和sPE组RORγc的蛋白表达水平明显上升;与PE组相比,sPE组患者RORγc的蛋白表达水平升高更显著,3组数据两两比较差异均有统计学意义(P<0.05)。结论:PD‑1/PD‑L1异常表达通过引起调节性T细胞/辅助性T细胞‑17免疫失衡参与子痫前期的发生及进展。
    2022,28(22):1710-1716, DOI: 10.13210/j.cnki.jhmu.20220816.002
    摘要:
    目的:探讨GC基因多态性与寻常性银屑病的关系。方法:采用靶基因捕获测序法对101例海南籍汉族寻常性银屑病患者与79例海南籍健康对照者的GC基因及其上、下游各2 kb进行全长测序,对最小等位基因频率大于1%且对照组Hardy⁃Weinberg平衡检验P值大于0.05的单核苷酸多肽性(SNP)在四种遗传模式下进行基于SNP的关联分析。采用生物信息学方法预测外显子区风险位点对基因功能的影响。结果:共检测到94个SNP,其中93个符合纳入标准。在基于SNP的关联分析中,有21个SNP(16个位于内含子、2个位于外显子及3个位于非翻译区)至少在一种遗传模式下为银屑病的易感位点(P<0.05),OR为0.289~2.295,95%CI为0.048~12.670。生物信息学预测显示位于外显子11上的 rs4588为非同义突变,可将苏氨酸转换为赖氨酸(SIFT Score=0.481、SIFT Score Pred为T),另一个位于外显子8上的rs4752A>G为同义突变,未导致氨基酸的改变。结论:GC基因与海南汉族寻常性银屑病易感性相关。
    2022,28(22):1717-1723, DOI: 10.13210/j.cnki.jhmu.20220817.003
    摘要:
    目的:探究甾醇氧⁃酰基转移酶 1(SOAT1)在肝细胞性肝癌组织中的表达情况,并探讨其联合多种标记物(HSP70、GS、CD10、CD34)在诊断肝细胞性肝癌中的应用可行性。方法:收集58例肝细胞性肝癌组织、配对癌旁肝组织及12例良性肝病变组织样本,制作组织芯片,免疫组织化学方法检测SOAT1、HSP70、GS、CD10、CD34的表达情况,并分析其表达相关性。结果:SOAT1在肝细胞性肝癌组织中表达上调(P<0.01),且表达水平与肝细胞性肝癌患者分化程度密切相关(P<0.05)。SOAT1与HSP70、GS、CD34表达相关(P<0.01);肝细胞性肝癌诊断阳性符合率分别为CD34 100.00%、GS 89.66%、HSP70 82.75%、CD10 63.79%、SOAT1 63.79%;而在癌旁组织中诊断阴性符合率分别为SOAT1 100.00%、CD10 98.27%、HSP70 96.55%、CD34 34.48%、GS 5.17%;良性肝病变组织中诊断阴性符合率分别为SOAT1 100.00%、CD10 83.34%、HSP70 100.00%、CD34 8.33%、GS 0%。以任意3种标记物阳性为诊断标准,不将SOAT1计入标记物行列,58例肝细胞性肝癌中有39例可被诊断,诊断率为67.24%;而将SOAT1计入标记物行列,58例肝细胞性肝癌中有48例可被诊断,诊断率可提高至82.76%。结论:SOAT1在肝细胞性肝癌中表达水平明显上调,且在低分化肝细胞性肝癌表达水平更高,提示SOAT1可作为肝细胞性肝癌的辅助诊断及预后判断指标之一。SOAT1、HSP70、GS、CD10及CD34 5种标记物中,敏感性较好为CD34及GS,而特异性较好则为SOAT1、CD10及HSP70。SOAT1与HSP70、GS、CD10、CD34联合应用,在肝细胞性肝癌的辅助诊断中有一定应用价值。
    2022,28(22):1724-1731, DOI: 10.13210/j.cnki.jhmu.20220711.001
    摘要:
    目的:分析视神经脊髓炎相关性视神经炎(neuromyelitis optical associated optic neuritis, NMO⁃ON)患者的临床特点,为防治该类疾病提供参考和依据。方法:对2016年1月~2019年12月就诊于中日友好医院的72例以ON为首发临床表现的NMO患者病历资料进行回顾性分析总结,包括一般资料、发病特点、病程进展、合并疾病、免疫学检验、治疗反应及预后等。结果:72例NMO⁃ON患者,发病年龄中位数33岁;男女比约为1∶5.54;病程中位数67个月,以“复发⁃缓解”为主。61.11%患者双眼先后累及,ON发病次数中位数为2次,ON二次发作时间中位数是3个月;1、3年复发率分别约55.56%和73.61%;91.67%患者单独ON起病,ON累及单眼者占81.94%;19.44%患者伴诱因,最常见为上呼吸道感染;15.28%患者伴系统性免疫性疾病,最常伴发干燥综合征及甲状腺疾病;75.64%患者首发视力小于0.1,抗水通道蛋白4抗体(aquaporin⁃4 immunoglobulin G,AQP4⁃IgG)状态(P=0.032,OR=2.55)和发病年龄(P=0.037,OR=3.93)是影响患者首发视力的独立危险因素;至末次随访时间,单侧致盲率约48.61%,致患者单侧致盲ON发作中位数为2次;73.61%患者出现其他神经系统的累及,脊髓(61.11%)是最常见的复发部位。80.00%(48/60)患者血清AQP4⁃IgG阳性;18例(25.00%)伴其他系统性免疫性抗体,最常见伴ANA抗体阳性。结论:NMO⁃ON患者首次发病多ON单独起病,单侧累及,女性中青年高发,漫长的病程多见双侧视神经受累及多次复发,AQP4⁃IgG状态和发病年龄是影响NMO患者首次发病视功能的独立危险因素,大多数患者AQP4⁃IgG血清阳性,部分患者伴发以干燥综合征和甲状腺疾病为代表的系统性免疫性疾病,该病临床高危,需要及早进行明确诊断和治疗干预。
    2022,28(22):1732-1740, DOI: 10.13210/j.cnki.jhmu.20210511.001
    摘要:
    目的:基于网络药理学探究骨碎补总黄酮治疗类风湿性关节炎(rheumatoid arthritis, RA) 的作用机制,为进一步的研究提供基础。方法:利用中药系统药理数据库和分析平台(TCMSP)获取骨碎补总黄酮的活性成分,通过TCMSP、Pubchem、SwissTargetPrediction和STITCH数据库预测骨碎补中有效黄酮类成分靶点,并通过Uniprot数据库获取标准靶点名称。通过GenBank、GeneCards、DisGeNET和OMIM数据库检索RA疾病相关靶点。获取骨碎补药物靶点-RA的交集基因,使用STRING数据库构建蛋白互作网络以预测核心蛋白,进行GO功能富集分析与KEGG通路富集分析。构建骨碎补总黄酮治疗RA调控网络,并将骨碎补总黄酮活性成分与预测得到的核心靶点进行分子对接。结果:从骨碎补总黄酮中筛选出木犀草素、柚皮素、山奈酚等活性化合物共10个,预测得到有效靶点210个,筛选RA相关靶点2 009个,得到骨碎补总黄酮-RA交集靶点123个。其中STAT3、MAPK1、MAPK3、AKT1、MAPK8、IL-6、TNF、MAPK14、IL-4、IL-2、VEGFA、IL-1β、MAPK9等可能为骨碎补总黄酮治疗RA的关键作用靶点。GO基因富集分析提示,转录调控及细胞因子活性调节可能是骨碎补总黄酮发挥RA治疗作用的关键途径。KEGG通路富集分析提示PI3K/AKT、IL-17、TNF-α、T细胞受体转导及Th17细胞分化调节等信号通路可能在骨碎补总黄酮治疗RA发挥重要作用。骨碎补总黄酮治疗RA调控网络提示,木犀草素与柚皮素可能是骨碎补总黄酮治疗RA的关键成分。分子对接提示木犀草素与核心靶点AKT1、PI3K和STAT3具有较好的亲和能力,可能通过改变分子构象进一步影响其磷酸化。结论:骨碎补总黄酮可能通过调控炎性相关细胞因子及相关传导途径,从而抑制RA的炎性反应,而PI3K/AKT通路可能是骨碎补总黄酮发挥治疗RA的重要途径。木犀草素是骨碎补总黄酮治疗RA的关键分子,并可能通过抑制相关核心蛋白的构象,进而调控磷酸化发挥下游途径作用。
    2022,28(22):1741-1747, DOI: 10.13210/j.cnki.jhmu.20210824.005
    摘要:
    目的:系统评价痰热清注射液辅助治疗心力衰竭合并肺部感染的临床疗效及安全性。方法:检索中国知网、中国生物医学文献数据库、中文科技期刊全文数据库、万方数据库、Cochrance Library、Web of Science和PubMed数据库,检索时间从建库至2021年8月,收集痰热清注射液治疗心力衰竭合并肺部感染的临床随机对照试验,由2位研究员独立筛选录入文献数据,运用RevMan5.4.1软件进行数据分析。结果:共纳入10篇文献,包含862例心力衰竭合并肺部感染患者,其中试验组有431例,对照组有431例。Meta分析结果显示,试验组与对照组相比,可提高临床总有效率[OR=4.56,95%CI(2.79,7.52),P<0.000 01],降低C-型反应性蛋白数值[MD=-7.55,95%CI(-11.40,-3.69),P=0.000 1],缩短纠正心衰的时间[OR=-4.04,95%CI(-4.59,-3.49),P<0.000 01],减少平均住院的天数[MD=-4.78,95%CI(-6.67,-2.89),P<0.000 01],两组不良反应发生率差异无统计学意义(P>0.05)。结论:痰热清注射液辅助治疗心力衰竭合并肺部感染,可提高临床总有效率,在降低C-型反应性蛋白数值、缩短纠正心衰的时间、减少平均住院的天数方面具有一定的优势,不良反应较小。但纳入研究数据总体质量偏低,尚需开展更多高质量的随机对照试验以增加循证依据。
    2022,28(22):1748-1754, DOI: 10.13210/j.cnki.jhmu.20210514.005
    摘要:
    柴胡是伞形科植物柴胡(Bupleurem chinense DC.)或狭叶柴胡(Bupleurem scorzonerifolium Willd.) 的干燥根,其广泛分布于东北、华北、西北、华东、湖北、四川等地。春、秋二季采挖,除去茎叶和泥沙,干燥。柴胡应用历史悠久,是最古老、最常用的中草药之一,具有较高的药用价值。 现代药理研究表明,柴胡具有抗癌、抗抑郁、抗炎、保护心脏、保护肝脏、保护肾脏等药理作用,并主要通过调节凋亡信号通路、炎症信号通路、神经内分泌系统、氧化应激信号通路、纤维化信号通路发挥作用。本文就近年来柴胡的现代药理作用进行文献综述,旨在为更好地研究开发和合理利用柴胡资源提供参考。
    2022,28(22):1755-1760, DOI: 10.13210/j.cnki.jhmu.20210430.004
    摘要:
    烧心是消化系统疾病最常见的胃肠道症状之一。在质子泵抑制剂广泛应用的背景下,仍有大量患者以抑酸治疗后,烧心持续不缓解为主诉就诊,部分被诊断为功能性烧心(functional heartburn,FH)。FH的诊断需要内镜活检术排除嗜酸性食管炎等黏膜异常病变,高分辨食管测压排除食管动力障碍性疾病,以及反流监测证实无反流相关性症状。FH的病理生理学机制仍不明确,可能涉及内脏高敏感和心理应激障碍,其治疗很大程度上仍为经验性治疗,临床医师应尽可能宽慰患者,避免手术治疗。尽管缺乏高质量临床证据支持,神经调节剂、中医药治疗以及心理干预可能是有效的方法。
    全选反选导出
    显示模式:
    2019,25(5):6-9, DOI:
    摘要:
    Objective: To study the effect of Baihe Dihuang Decoction in improving anxiety and its mechanism of action. Methods: Male mice were randomly divided into 5 groups: blank control group, Baihe Dihuang Decoction high, medium, low-dose group and diazepam group. After continuous intragastric administration for 14 d, the behavioral test of the mouse elevated plus maze experiment and opening test was performed. The brain tissue GABA and Glu content was measured by immunosorbent assay (ELISA). Results: Compared with the blank control group, the middle and high doses of Baihe Dihuang Decoction could increase the time and frequency of movement of the mice in the open cross labyrinth in the open arm and increase the number of times that the mouse entered the central area in the opening test. The anti-anxiety effect also showed an increase in the brain tissue GABA content in mice, ignificantly decreased Glu contents in mice. Conclusion: Baihe Dihuang Decoction has some anxiolytic effect, and antianxiety effect may be related to increasing brain tissue GABA content, decreased Glu contents in mice.
    2019,25(5):1-5, DOI:
    摘要:
    Objective: The current study aims to identify the effects of exogenous application of pulsed electromagnetic fields on skin wound healing in diabetic rats, and thus provides experimental evidence for its more scientific clinical application in the future. Methods: The diabetic animal models were established via intraperitoneal injection of streptozotocin in 4-monthold male rats. Fourth eight rats were randomly assigned into the diabetes group (DM) and diabetes coupled with pulsed electromagnetic fields (DM+PEMF) group. Another 24 normal rats were used as the blank control group (Control). Then, all the rats in the three groups were subjected to dorsal surgery for the establishment of soft tissue wound model (circular wound with the diameter of 2 cm). Then, the rats in the DM+PEMF group were subjected to pulsed electromagnetic fields stimulation. Then, 6 rats in each group were sacrificed at Day 5, 12 and 19 post surgery. The glucose levels, wound closure, wound healing time and tissue tensile strength were examined and analyzed. Results: Pulsed electromagnetic fields significantly increased the wound closure rate in diabetic rats at Day 5, 12 and 19 post surgery, decreased overall wound healing period in diabetic rats, and also enhanced tissue tensile strength in diabetic rats at Day 5, 12 and 19 post surgery. Conclusion: Low-intensity pulsed electromagnetic fields can significantly accelerate diabetic wound healing process, and also improve diabetic tissue repair capacity. This study may be helpful for providing more scientific and reasonable experimental evidence for the treatment of pulsed electromagnetic fields on diabetic wound healing in clinics.
    2019,25(5):69-72, DOI:
    摘要:
    Gastric cancer is one of common malignant tumors in clinic. It has high incidence, high mortality and poor prognosis. Generally, gastric cancer is divided into early gastric cancer (EGC) and advanced gastric cancer. The 5-year survival rate of EGC is higher than that of advanced gastric cancer. In recent years, with the continuous development of science and technology, a variety of medical instruments and technologies have been updated, greatly promoting the detection rate of EGC, reducing the rate of EGC missed diagnosis. This article reviews the progress of EGC endoscopic diagnosis.
    2019,25(5):61-64, DOI:
    摘要:
    Objective: To investigate the effects of routine rehabilitation therapy combined with low frequency head stimulation on monoamine neurotransmitters, neuron-specific enolase (NSE), endothelin-1 (ET-1) and cerebral hemodynamics in children with cerebral palsy. Methods: From January 2017 to June 2018, 110 children with cerebral palsy were randomly divided into observation group (55 cases) and control group (55 cases). The control group received routine rehabilitation treatment, while the observation group received low-frequency head stimulation on the basis of routine rehabilitation treatment. The changes of dopamine (DA), norepinephrine (NE), serotonin (5-HT), NSE, ET-1 levels and mean blood flow velocity of anterior cerebral artery (ACA), middle cerebral artery (MCA), posterior cerebral artery (PCA) were compared in two groups. Results: Before treatment, there was no significant difference in DA, NE and 5-HT levels in two groups. After treatment, DA, NE and 5-HT levels in the observation group were (192.23±22.71) ng/mL, (98.02±11.71) ng/L, (210.07±25.03) ng/L, and in the control group. the DA, NE, 5-HT levels were (147.06±17.02) ng/mL, (83.07±11.15) ng/L, and (171.88±20.45) ng/L, respectively. The DA, NE and 5-HT levels in two groups were higher than those before treatment, and DA, NE and 5-HT levels in the observation group were higher than those in the control group. Before treatment, there was no significant difference in NSE and ET-1 levels between the two groups. After treatment, the NSE and ET-1 levels in the observation group were (7.97±2.07) μg/L and (41.01±10.07) pg/ mL, and the NSE and ET-1 levels in the control group were (10.38±3.02) μg/L, (58.46±15.02) pg/ mL, respectively. the NSE and ET-1 in two groups were lower than those before treatment, and the NSE and ET-1 of the observation group were lower than the control group. Before treatment, there was no significant difference in mean blood flow velocity between ACA, MCA and PCA. After treatment, the mean blood flow velocities of ACA, MCA, and PCA in the observation group were (46.88±7.72) cm/s, (59.85±10.18) cm/s, and (49.15±7.02) cm/s, respectively, which was significantly higher than before treatment and higher than that of the control group in the same period. Conclusion: Conventional rehabilitation combined with low-frequency electrical stimulation of the head can effectively increase the content of monoamine neurotransmitters in children with cerebral palsy, enhance cerebral blood circulation, and reduce brain damage
    2019,25(5):65-68, DOI:
    摘要:
    Objective: To study the refractory factors associated with schizophrenia. Methods: 200 patients with refractory schizophrenia and 200 patients with non-refractory schizophrenia were selected. The CYP series of genes CYP1A2, CYP3A4 and CYP2D6 were detected by drug gene, and the rapid metabolic probabilities of the three genes were compared and analyzed. 200 patients with refractory schizophrenia were randomly divided into two groups: the combined drug treatment group and the single drug treatment group. The results were compared between the treatment of 0W and 4W for drug gene detection, 3 genes fast metabolizing type and BPRS scale. analysis. Results: The rapid metabolizing probability and non-refractory difference of CYP1A2, CYP3A4 and CYP2D6 genes in patients with refractory schizophrenia were significant. The comparison of fast metabotropic probabilities of CYP1A2, CYP3A4 and CYP2D6 genes in patients treated with 4W after treatment There was no significant difference in the single drug treatment group. The BPRS scale score was significantly higher in the drug-treated group than in the single-drug group. After logistic regression analysis, the refractory characteristics of schizophrenia and The CYP series of genes CYP1A2, CYP3A4, and CYP2D6 are rapidly metabolized. Conclusion: CYP series of genes CYP1A2, CYP3A4, CYP2D6 fast metabolites are related factors of refractory schizophrenia, antipsychotic drugs combined with CYP enzyme inhibitor treatment can improve the efficacy.
    2019,25(5):73-76, DOI:
    摘要:
    Cervical laminoplasty surgery is an alternative to laminectomy. It can increase the space of the spinal cord available by raising the lamina, and achieve the indirect decompression effect to treat cervical spondylosis. Various techniques of laminoplasty have since been developed after two prototype techniques: Hirabayashi’s open-door laminoplasty and Kurokawa’s double-door laminoplasty. Several studies report superior biomechanical stability of the cervical spine after laminoplasty compared with laminectomy. Recent development in laminoplasty is preservation of muscle attachment, which enabled dynamic and static stabilization of the cervical spine by neck extensor muscles. After treatment with new laminoplasty techniques with active postoperative neck muscle exercises, postoperative instability, kyphosis, axial neck pain, and loss of ROM become minimal. The development,complications and future trends of cervical laminoplasty are summarized below.
    2019,25(5):41-44, DOI:
    摘要:
    Objective: To investigate the effects of gastrodin on neurotransmitter effect, stress response, blood vessel and nerve function in migraine patients. Methods: A total of 90 patients with migraine admitted to our hospital from September 2016 to December 2017 were randomly divided into observation group and control group with 45 cases in each group. The control group was treated with flunarizine hydrochloride, while the observation group was treated with gastrodin based on the control group. The levels of neurotransmitter effect[including 5-serotonin (5-HT) and tumor necrosis factor alpha (TNF-alpha)], stress response[oxidized low density lipoprotein (ox-LDL) and paraoxonase-1 (PON-1)], vascular function[including nitric oxide (NO), endothelin-1 (ET-1)] and neurological function[including high brain-derived neurotrophic factor (BDNF) and calcitonin gene-related peptide (CGRP)] related indexes were observed and compared between the two groups. Results: The two groups showed significant changes in transmitter effect, stress response, blood vessel and nerve function. Before treatment, there was no significant difference in the levels between the two groups. After treatment, the levels of TNF-a, ox-LDL, NO, ET-1, BDNF and CGRP in the two groups were significantly lower than those before treatment, while the levels of 5-HT and PON-1 were significantly higher than those before treatment. And after treatment, the levels of TNF-a, ox- LDL, NO, ET-1, BDNF and CGRP in the observation group were significantly lower than those in the control group, while the levels of 5-HT and PON-1 were significantly higher than those in the control group. Conclusions: Gastrodin treatment can regulate oxidative stress level and improve vascular and neurological function in migraine patients.
    2019,25(5):49-52, DOI:
    摘要:
    Objective: To observe the effect of Hegu neediling method combined with tendon-stretching and plucking massage on trigger point in the treatment of acute thoracodorsal myofascitis. Methods: A total of 84 patients with acute thoracodorsal myofascitis who met the diagnostic and inclusion criteria were assigned into the treatment group and the control group randomly, the treatment group was treated with Hegu neediling method combined with tendonstretching and plucking massage, while the control group was treated with conventional electroacupuncture. Two groups of patients were treated for two weeks. VAS score and ODI were compared before treatment, after the first treatment and after the treatment course, and the clinical efficacy was evaluated after treatment. Results: The total effective rates of the treatment group and the control group were 97.62% and 80.95%, respectively. The treatment group was significantly better than the control group. The VAS and ODI scores of the treatment group decreased after the first treatment and the end of the treatment, and the treatment group was significantly better than the control group. Conclusion: Hegu neediling method combined with tendon-stretching and plucking massage can relieve pain and improve limb dysfunction in patients with acute thoracodorsal myofascitis.
    2019,25(5):26-30, DOI:
    摘要:
    Objective: To investigate the correlation between the change in expression of the tumor associated macrophage (TAM) markers CD68 and CD206 and the clinicopathological parameters. Methods: We retrospectively analyzed 28 PGI-DLBCL patients by immunohistochemical stainning. The surface expression of biomarkers CD68 and CD206 on TAMs in samples obtained at diagnosis was compared with that in specimens obtained at first relapse. Based on the change in expression levels of CD68 and CD206 from diagnosis to relapse, patients were categorized into three groups: “increased”, “stable” and “decreased”. Results: A significant difference was observed between the three groups with respect to CD68 and CD206 expressions (P=0.012) and there existed a consistency between the two (P=0.008). The three groups showed a negative correlation with age, sex, stage, B symptoms, diagnosis, ECOG performance status, and IPI scores, while a positive correlation was observed with the site of lesion of CD206 expression (P=0.032). The time to relapse (TTR) in the three groups of CD68 and CD206 was not significantly different (P=0.23, P=0.818). Conclusion: Our study suggests that increase in CD206 and decrease in CD68 expression at relapse indicates M1-TAM polarization into M2-TAM, which plays an important role in PGI-DLBCL relapse. Furthermore, M2-TAM polarization may be associated with the site of Primary gastric diffuse large B lymphoma.
    2019(11):805-809, DOI: 10.13210/j.cnki.jhmu.20190404.002
    [摘要] (555) [HTML] (0) [PDF 3.18 M] (213)
    摘要:
    目的:探讨鹰嘴豆芽素A对宫颈癌细胞增殖及迁移能力的调控作用及其可能的分子机制。方法:体外培养Hela细胞,以不同质量浓度的鹰嘴豆芽素A处理宫颈癌Hela细胞,CCK-8法检测鹰嘴豆芽素A对宫颈癌细胞的增殖影响;划痕实验检测对细胞迁移的影响;RT-PCR法检测鹰嘴豆芽素A对Bcl-2 /Bax mRNA水平表达的变化。结果:鹰嘴豆芽素A能够抑制Hela细胞的增殖和迁移,呈现剂量和时间依赖性(P<0.05);质量浓度为10、20、40、80、160 μmol/L鹰嘴豆芽素A处理Hela细胞24 h、48 h后,抑制Hela细胞增殖和迁移速率明显上升,并且高于对照组(P<0.05);促凋亡蛋白Bax表达水平升高,抗凋亡蛋白Bcl-2表达水平降低,呈剂量依赖性。结论:鹰嘴豆芽素A能够抑制宫颈癌Hela细胞的增殖和迁移,其作用机制可能与下调Bcl-2,上调Bax有关。
    2019,25(5):45-48, DOI:
    摘要:
    Objective: To explore the effect of selenium supplementation on thyroid function, immune response and oxidative stress in HT patients with normal thyroid function. Methods: A total of 120 Hashimoto's thyroiditis patients with normal thyroid function admitted to our hospital from June 2016 to March 2018 were randomly divided into treatment group and control group with 60 cases in each group. The control group was given oral placebo, while the treatment group was given selenium yeast. The expression levels of thyroid function [including thyroid stimulating hormone (TSH), free triiodothyronine (FT3) and free thyroxine (FT4)], immune response [including thyroid peroxidase antibody (TPOAb), thyroglobulin antibody (TGAb), interferon-γ (IFN-γ), interleukin-4 (IL-4)] and oxidative stress [including malondialdehyde (MDA), glutathione peroxidase (GPx) and superoxide dismutase (SOD)] were observed and compared between the two groups. Results: The two groups showed significant changes in auxiliary diagnosis, immune response and oxidative stress. Before treatment, there was no significant difference in the levels between the two groups. After treatment, there was no significant difference in the above indexes between the control group and that before treatment. There was no significant difference in thyroid function related indexes between the treatment group and the control group. The levels of TPOAb, TGAb, IFN-γ and MDA in the treatment group were significantly lower than those before treatment and in the control group, while the levels of IL-4, GPx and SOD were significantly higher than those before treatment and in the control group. Conclusions: Selenium supplementation can stabilize thyroid function, reduce the level of thyroid antibody production, and improve immune response and oxidative stress response in HT patients.
    2019,25(5):37-40, DOI:
    摘要:
    Objective: To study the effect of shexiangbaoxin pill combined with tirofiban hydrochloride on related factors in elderly patients with acute coronary symdrome. Methods: A total of 96 elderly patients with acute coronary symdrome in our hospital from January 2015 to May 2018 were enrolled in this study. The subjects were divided into the control group (n=48) and the treatment group (n=48) randomly. The control group were treated with tirofiban hydrochloride, the treatment group were treated with shexiangbaoxin pill combined with tirofiban hydrochloride, and both the two groups were treated for 2 weeks. The PT, TT, APTT and serum FIB, CK-MB, cTnT, vWF, ET-1, NO, sICAM-1, MMP-9, hs-CRP of the two groups before and after treatment were compared. Results: There were no significantly differences of the PT, TT, APTT and serum FIB, CK-MB, cTnT, vWF, ET-1, NO, sICAM-1, MMP-9, hs-CRP of the two groups before treatment. The PT, TT, APTT and serum NO of the two groups after treatment were significantly higher than before treatment, the serum FIB, vWF, ET, sICAM-1, MMP-9, hs-CRP of the two groups after treatment were significantly lower than before treatment, and that of the treatment group after treatment were significantly better than the control group. The serum CK-MB, cTnT of the two groups after treatment were significantly higher than before treatment, but that of the treatment group after treatment were significantly lower than the control group. Conclusion: Shexiangbaoxin pill combined with tirofiban hydrochloride can significantly improve the blood coagulation function and reduce the Myocardial injury, vascular endothelial damage, inflammation of the elderly patients with acute coronary symdrome, and it was worthy clinical application.
    2019,25(5):31-36, DOI:
    摘要:
    Objective: To systematically evaluate the effects of sleep status on renal function in patients with chronic kidney disease (CKD). Methods: To search the relevant literature related to the effects of sleep status on renal function of CKD patients on PubMed database, EMBase database, the Cochrane Library database, CNKI database, Chinese Biomedical Literature Database, VIP and Wanfang database from the initial to June 2018, all literature that met the criteria were included. According to the type of studies, the quality of the literature was evaluated by NOS scale in the cohort study and AHRQ scale in the cross-sectional study, and systematically evaluated the outcome indicators, the main outcome indicators were estimated glomerular filtration rate (eGFR) and endogenous creatinine clearance rate (Ccr), while the secondary indicators were Pittsburgh Sleep Quality Index (PSQI), Sleep Quality (SQ), Serum Creatinine (Scr), Hemoglobin (Hb), Albumin (ALB) and Urine Protein/Creatinine Ratio (UPCR). Results: Four literature and one meeting abstract were included in this study, of which four were cohort studies, three of them the NOS quality evaluations were high, one of them was medium, the remaining one was cross-sectional study, and the AHRQ quality evaluation was medium. This study shows that sleep status has a certain correlation with renal function. Shorter sleep time or poor sleep quality can lead to deterioration of renal function. Among them, the research data of Sabbatinit research team in Italy showed that Ccr gradually decreased with the increased of the PSQI; studies of Cohen research team and the Ricardo research team in the United States showed that eGFR decreased with the increased of the PSQI; the study of Kumar research team in the United States showed that the lower SQ , the worse renal function; the study of Knutson’ research team in British showed that the shorter sleep time, the lower eGFR. In addition, studies showed that sleep index also has influence on Hb, ALB, Scr, UPCR and other indicators. Conclusion: Sleep status can affect the renal function of CKD patients in different degrees. Shorter sleep time and poor sleep quality will damage renal function and accelerate the progress of CKD.
    2019(11):815-817, 825, DOI: 10.13210/j.cnki.jhmu.20190409.003
    [摘要] (456) [HTML] (0) [PDF 2.52 M] (209)
    摘要:
    目的:观察注意缺陷多动障碍(ADHD)模型动物自发性高血压大鼠(SHR)血清皮质酮(CORT)、促肾上腺皮质激素(ACTH)、促肾上腺皮质激素释放激素(CRH)水平及下丘脑糖皮质激素受体(GR)、糖皮质激素受体(MR)、CRH及海马GR、MR mRNA的表达情况,探讨SHR大鼠下丘脑-垂体-肾上腺轴(HPA)功能及相关影响因素。方法:以幼年SHR大鼠为ADHD模型组、Wistar京都大鼠(WKY)为对照组,采用ELISA法检测血清CORT、ACTH、CRH水平,RT-PCR法检测大鼠下丘脑GR、MR、CRH及海马GR、MR mRNA的表达水平。结果:SHR大鼠血清CORT、ACTH水平明显低于WKY大鼠(P<0.01),血清CRH水平明显高于WKY大鼠(P<0.01),下丘脑及海马GR、MR mRNA的表达水平明显高于WKY大鼠(P<0.01),而下丘脑CRH mRNA表达则低于WKY大鼠(P<0.01)。结论:SHR大鼠HPA轴呈低反应性,可能与CRH启动障碍及下丘脑和海马GR、MR mRNA 高表达相关。
    2019(11):810-814, DOI: 10.13210/j.cnki.jhmu.20190404.003
    [摘要] (515) [HTML] (0) [PDF 2.81 M] (205)
    摘要:
    目的:探讨伊伐布雷定(IVA)对心肌梗死大鼠心肌细胞Notch和 NF-κB信号通路的影响。方法:采用结扎冠状动脉左前降支建立心肌梗死模型,将存活大鼠随机分为模型组(MI组,n=8)和治疗组(IVA组,n=8)。以相同部位穿线但不结扎冠状动脉左前降支的大鼠作为对照组(CON组,n=8)。 IVA给药28 d。检测所有大鼠血流动力学和心功能指标:心率(HR)、收缩压(SBP)、舒张压(DBP)、平均动脉压(MAP)、左室收缩压(LVSP)、左室舒张末压(LVEDP)和左室内压最大上升和下降速率(±dp/dt);左室重量指数、左室截面直径和心肌梗死面积;RT-PCR检测大鼠心肌细胞Notch信号通路成分mRNA的表达水平(Notch-1、Dll-4、Hes-1);Western-blot检测DICD-1蛋白和P65蛋白表达水平。组间比较采用单因素方差分析,两两比较采用 SNK法。结果:MI组SBP、 DBP、MAP、LASP、LVEDP和±dp/dt均低于对照组(P<0.05);而IVA上述指标均高于MI组(P<0.05)。MI组左室重量指数和左室截面直径明显大于对照组(P<0.05),但小于IVA组(P<0.05)。MI组Notch-1 mRNA表达水平明显高于对照组(P<0.05),但低于IVA组(P<0.05)。3组Dll-4和Hes-1 mRNA表达水平的比较差异无统计学意义(P>0.05)。MI组心肌细胞NICD-1蛋白和P65蛋白表达水平明显高于CON组(P<0.05),但是低于IVA组(P<0.05)。结论:IVA可能通过Notch和 NF-κB信号通路发挥改善心肌梗死大鼠心功能和抑制心室重构的作用。
    2019(11):829-833, DOI: 10.13210/j.cnki.jhmu.20190426.003
    [摘要] (336) [HTML] (0) [PDF 3.07 M] (205)
    摘要:
    目的:探究脑内多巴胺神经递质表达对神经内科住院患者睡眠障碍的影响。 方法:选取我院神经内科收治的34例睡眠障碍患者和同期30例志愿者作为研究对象,分别为观察组和对照组,64例入选人员均于2周前停止服用咖啡、酒等刺激性饮料,采用匹兹堡睡眠质量指数量表(PSQI)、汉密顿抑郁量表(HAMD)检测睡眠质量和抑郁程度,同时进行脑部MRI检查,2 d后进行PET/CT扫描11C-雷氯必利标记的脑内多巴胺神经递质与受体结合情况。采用SPSS分析多巴胺神经递质与睡眠障碍相关指标的相关性。 结果:观察组HAMD、PSQI评分均显著高于对照组,差异具有统计学意义(P<0.05)。PET/CT扫描11C-雷氯必利多分布于伏隔核、尾状核、壳核。观察组伏隔核、尾状核、壳核多巴胺结合力均显著低于对照组,差异具有统计学意义(P<0.05)。采用pearson相关性分析,观察组病程与伏隔核、尾状核、壳核呈负相关(r=-0.754、-0.717、-0.728,P<0.05),而HAMD、PSQI评分与伏隔核、尾状核、壳核无相关性(P>0.05)。 结论:睡眠障碍患者多巴胺受体结合降低,同时与睡眠障碍的病程相关,提示多巴胺表达水平降低和睡眠障碍具有临床意义。
    2019,25(5):10-13, DOI:
    摘要:
    Objective: To investigate the effect of dexmedetomidine against liver ischemia-reperfusion injury in rats by reducing endoplasmic reticulum stress. Methods: A total of 24 adult male Sprague-Dawley rats were randomly divided into sham operation group (6 rats, Sham group), liver ischemia-reperfusion injury group (6 rats, I/R group), and liver ischemia-reperfusion injury +dexmedetomidine pretreatment group (6 rats, I/R +Dex pretreatment group) (25 μg/kg intraperitoneally 30 min before ischemia), and liver ischemia-reperfusion injury +dexmedetomidine post-treatment group (6 rats, I/R +Dex post-treatment group) (25 μg/kg intraperitoneally 30 min after reperfusion). Hepatic ischemia-reperfusion injury model was performed by after clamping the hepatic hilum for 30 min and reperfusion for 6 h. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were measured by automatic biochemical analyzer. Malondialdehyde (MDA) and superoxide dismutase (SOD) activity were detected by enzyme-linked immunosorbent assay (ELISA). The pathological changes of each group were observed by HE staining. The expression of endoplasmic reticulum stress (P-PERK, P-IRE1α, CHOP) were detected by Western blot. Results: Compared with the Sham group, the serum levels of ALT and AST were significantly higher in the I/R group, compared with the I/R group, the serum ALT and AST in I/R+Dex pretreatment group and the I/R+Dex post-treatment group were significantly reduced; Compared with the Sham group, MDA activity in the liver tissue of the I/R group was significantly increased, while the SOD activity was significantly decreased, and the pathological score of the liver tissue was significantly increased; Compared with the I/R group, MDA activity and liver histopathology scores in I/R+Dex pretreatment group and the I/R+Dex post-treatment group were decreased, while SOD activity increased; The expression of P-PERK, P-IRE1α, CHOP in the I/R group were significantly higher than that in the Sham group, while the expression of the above indicators were reduced in I/R+Dex pretreatment group and the I/R+Dex post-treatment group. Conclusion: Dexmedetomidine can significantly attenuated liver ischemia-reperfusion injury in rats, which may be related to the reduction of endoplasmic reticulum stress.
    2019,25(5):22-25, DOI:
    摘要:
    Objective: To explore the best time for progesterone supplementation in AID ovulation induction cycles by Letrozole. Methods: The data analysed in this study were collected from 509 patients who were performed AID (Artificial Insemination by Donor) administrated letrozole (LE) between 2014.8-2015.7. All patients were randomly divided into 4 groups by the time of progesterone administrated, including experimental group and the control group. The experimental group was divided into group 1-72 h after ovulation, group 2-48 h after ovulation, group 3-24 h after ovulation and control group—without administrated LE. The gestation and live birth rate were evaluated by monitoring vaginal ultrasound and HCG blood value 14 d after AID. Results: The pregnancy rate with administrated progesterone added 72 h after ovulation was 31.9%, which was significantly higher than those of other groups, the same situation as groups added progesterone was significantly higher than the control group. However, there was no significant difference in the numbers of abortions among the four groups. The LBR of group 4 was significantly lower than that of group 1. Conclutions: Progesterone administrated 72 h after ovulation can promoted the gestation rate, but did not affect the rate of miscarrage .
    2019,25(5):18-21, DOI:
    摘要:
    Objective: To study the expression and significance of KAI1/CD82, p27 proteins in gastric carcinoma. Methods: The expressions of KAI1/CD82, p27 proteins were detected by immunohistchemistry S-P method in 58 cases of gastric carcinoma tissues and 23 normal gastric tissues. Results: The positive rates of KAI1/CD82 and p27 proteins in gastric carcinoma tissues were 29.3% and 34.4%, but 90% and 85% respectively in normal gastric tissue. And there is a significant difference between the two groups. The expression level of KAI1/CD82 and p27 proteins was significantly related to tumor invasive depth, grade of tumor differentiation, the clinical stage and lymph node metastasis, And has nothing to do with age or gender. There was a positive correlation between the expression of KAI1/CD82 and P27 in gastric cancer tissues. Conclusion: The expression of KAI1/CD82 and p27 protein may be involved in the initiation and development in gastric carcinoma, and the combined detection of KAI1/CD82 and p27 proteins may be significant in predicting the invision and metastasis of gastric carcinoma.
    2019(11):801-804, 809, DOI: 10.13210/j.cnki.jhmu.20190416.002
    [摘要] (675) [HTML] (0) [PDF 3.79 M] (202)
    摘要:
    目的:利用壮药南酸枣树皮水煎剂干预慢性难愈合创面模型大鼠,探究其疗效机制。方法:采用全层皮肤缺损法改进慢性难愈合创面大鼠模型,将40只大鼠随机分为空白对照组、模型组、湿润烧伤膏组、南酸枣树皮组,空白对照组和模型组外敷生理盐水,湿润烧伤膏组采用皮肤再生医疗技术,南酸枣树皮组外敷南酸枣树皮水煎剂。12 d后取全层皮肤组织进行超微病理检测,利用RT-PCR技术观察PTEN、VEGF mRNA的变化情况。结果:经12 d药物干预后,湿润烧伤膏组、南酸枣树皮组皮肤细胞器的形态结构基本恢复。第12天湿润烧伤膏组、南酸枣树皮组分别与第1天相比,均出现PTEN mRNA水平降低、VEGF mRNA水平增高,差异具有统计学意义(P<0.05);第12天湿润烧伤膏组、南酸枣树皮组分别与模型组相比,均出现PTEN mRNA水平降低、VEGF mRNA水平增高,差异具有统计学意义(P<0.05);而湿润烧伤膏组与南酸枣树皮组相比差异无统计学意义 (P>0.05)。结论:壮药南酸枣树皮能显著改善慢性难愈合创面导致的细胞结构异常,调控PTEN介导的信号通路传导效应,改善损伤病灶,促进创面组织修复。

微信公众号二维码

手机版网站二维码

关闭