Expression of FZD10 in gastric cancer and its effect on gastric cancer cells
Objective: To investigate the expressions of FZD10 and its effect on gastric cancer cells. Methods: The expression of FZD10 in gastric cancer tissues and adjacent cancer tissues was detected by immunohistochemistry；The gastric cancer cell lines AGS and MGC-803 were transfected with siRNA, and then use Western-blot to analysis the transfection efficiency; The proliferation and invasion ability of gastric cancer cell was analyzed by CCK-8 and wound heal. Results: The expression of FZD10 in gastric cancer was significantly higher than that in adjacent tissues (P<0.05). Compared with the blank control group, the proliferation and cell invasion ability of siRNA transfected with AGS and MGC-803 decreased significantly (P<0.05), while the blank control group and empty control group No significant difference between the negative control groups (P>0.05). Conclusion: The expressions of FZD10 significantly up-regulated in gastric cancer, and silencing its expression will affect the proliferation and invasive ability of gastric cancer cells.
Natural Science Foundation of Hubei Province(2016CFB668).