Journal of Hainan Medical University
Volume ,2023 Issue 5
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- Neuroprotective effect of Phyllanthus urinaria extract on ischemic brain injury in rats
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2023(5):321-330, DOI: 10.13210/j.cnki.jhmu.20221229.002
Abstract:
Objective： To study the intervention effect of Zhuangfang Soft Liver and Fibrous Granules on CCl4 compound factor‑induced liver fibrosis rat model and its effect on α‑SMA and E‑Cadherin protein expression.Methods： Forty‑eight male Wistar rats were randomly divided into a blank group， a pathological model group and a group of 16 rats in each group. The rats in the pathological model group and the pellet group were injected subcutaneously with 40% carbon tetrachloride （CCl4） oil combined with peanut oil to establish an animal model of rat liver fibrosis， and the pellets were administered by gavage three times a week for 8 weeks from the 5th week. The expression of oxidative stress oxidase dismutase （SOD） and malondialdehyde （MDA） was detected by enzyme‑linked immunoassay （ELISA）， and the expression of α‑smooth muscle actin （α‑SMA） and E‑calmodulin （E‑Cadherin） was detected by RT‑PCR and Western Blot. expression. Results：Compared with the blank group， the serum ALT and AST levels of the rats in the pathological model group were significantly increased， and the serum ALT and AST levels of the rats in the soft liver and fibrous pellet group were significantly decreased （P<0.001）. In the pathological model group， the lobular structure of liver tissue was mutilated and numerous liver fibrous tissues were abnormally proliferated， and the collagen fibers formed by the proliferation were confluent into dense fibrous intervals， thus turning into pseudolobular structure， and a large number of fatty degenerated hepatocytes of different shapes and sizes appeared inside the pseudolobular structure， and the diffuse infiltration of inflammatory cells was significant. Compared with the pathological model group， the degree of destruction of hepatic lobular structure was significantly reduced in the LBP group， and there were different degrees of proliferation of collagen fibrous tissue concentrated in the area from the central vein to the confluent area， and the fibrous septum was relatively loose， with a small amount of inflammatory cell infiltration， and the degree of infiltration was reduced compared with the pathological model group. Compared with the pathological model group， the SOD level was significantly higher in the group of liver softener and fibrous pellets compared with the pathological model group and the blank group （P<0.001）， the MDA level was significantly lower in the group of liver softener and fibrous pellets compared with the pathological model group （P<0.001）， the expression level of α‑SMA was significantly lower in the group of liver softener and fibrous pellets （P<0.001）， and the expression level of E‑Cadherin was significantly higher in the group of liver softener and fibrous pellets （P<0.001）. Conclusion： Soft Liver Fibro Pellets can effectively inhibit liver fibrous tissue proliferation and reduce the serum ALT and AST levels in rats with CCl4 combined with peanut oil compound solution induced liver fibrosis， and its anti‑liver fibrosis mechanism may be through protecting liver function， reducing the level of oxidative stress， down‑regulating the expression of α‑SMA protein， up‑regulating the expression of E‑cadherin protein， and inhibiting epithelial‑mesenchymal transition epithelial mesenchymal transformation （EMT） and thus exerting therapeutic effects on CCl4 compound‑induced hepatic fibrosis in rats.
Objective： To study the intervention effect of Zhuangfang Soft Liver and Fibrous Granules on CCl4 compound factor‑induced liver fibrosis rat model and its effect on α‑SMA and E‑Cadherin protein expression.Methods： Forty‑eight male Wistar rats were randomly divided into a blank group， a pathological model group and a group of 16 rats in each group. The rats in the pathological model group and the pellet group were injected subcutaneously with 40% carbon tetrachloride （CCl4） oil combined with peanut oil to establish an animal model of rat liver fibrosis， and the pellets were administered by gavage three times a week for 8 weeks from the 5th week. The expression of oxidative stress oxidase dismutase （SOD） and malondialdehyde （MDA） was detected by enzyme‑linked immunoassay （ELISA）， and the expression of α‑smooth muscle actin （α‑SMA） and E‑calmodulin （E‑Cadherin） was detected by RT‑PCR and Western Blot. expression. Results：Compared with the blank group， the serum ALT and AST levels of the rats in the pathological model group were significantly increased， and the serum ALT and AST levels of the rats in the soft liver and fibrous pellet group were significantly decreased （P<0.001）. In the pathological model group， the lobular structure of liver tissue was mutilated and numerous liver fibrous tissues were abnormally proliferated， and the collagen fibers formed by the proliferation were confluent into dense fibrous intervals， thus turning into pseudolobular structure， and a large number of fatty degenerated hepatocytes of different shapes and sizes appeared inside the pseudolobular structure， and the diffuse infiltration of inflammatory cells was significant. Compared with the pathological model group， the degree of destruction of hepatic lobular structure was significantly reduced in the LBP group， and there were different degrees of proliferation of collagen fibrous tissue concentrated in the area from the central vein to the confluent area， and the fibrous septum was relatively loose， with a small amount of inflammatory cell infiltration， and the degree of infiltration was reduced compared with the pathological model group. Compared with the pathological model group， the SOD level was significantly higher in the group of liver softener and fibrous pellets compared with the pathological model group and the blank group （P<0.001）， the MDA level was significantly lower in the group of liver softener and fibrous pellets compared with the pathological model group （P<0.001）， the expression level of α‑SMA was significantly lower in the group of liver softener and fibrous pellets （P<0.001）， and the expression level of E‑Cadherin was significantly higher in the group of liver softener and fibrous pellets （P<0.001）. Conclusion： Soft Liver Fibro Pellets can effectively inhibit liver fibrous tissue proliferation and reduce the serum ALT and AST levels in rats with CCl4 combined with peanut oil compound solution induced liver fibrosis， and its anti‑liver fibrosis mechanism may be through protecting liver function， reducing the level of oxidative stress， down‑regulating the expression of α‑SMA protein， up‑regulating the expression of E‑cadherin protein， and inhibiting epithelial‑mesenchymal transition epithelial mesenchymal transformation （EMT） and thus exerting therapeutic effects on CCl4 compound‑induced hepatic fibrosis in rats.
2023(5):331-339, DOI: 10.13210/j.cnki.jhmu.20221206.003
Abstract:
Objective： To investigate the role and mechanism of mTOR in sorafenib‑ameliorated liver fibrosis by inducing hepatic stellate cells （HSC） ferroptosis. Methods： The liver fibrosis models of C57BL/6 male mice were induced with carbon tetrachloride （CCl4） and randomly divided into normal group， model group， and sorafenib low （2.5 mg/kg）， medium （5 mg/kg）， and high （10 mg/kg） dose groups. Except for the normal group， the remaining four groups were treated with intraperitoneal injection of CCl4 for 8 weeks to establish liver fibrosis models. In addition， the corresponding concentrations of sorafenib were administered during the fourth week of modeling. Western blot was used to detect the expression of mTOR and p‑mTOR protein in liver tissues. In vitro experiments， HSC‑T6 cells were activated by PDGF‑BB and then treated with sorafenib （5 μM， 10 μM， 20 μM）， an mTOR inhibitor and activator. CCK8 was used to detect HSC‑T6 cell viability， Western blot detected the protein expression of α‑SMA， COL1α1， GPX4， mTOR and p‑mTOR. The levels of serum iron， GSH， and MDA were measured， and the intracellular ROS level was measured by 2'，7'‑dichlorofluorescein diacetate and dihydroethidium.Results： In vitro results showed that sorafenib significantly decreased the protein expression of α‑SMA， COL1α1， GPX4 and p‑mTOR， and decreased the level of GSH and increased the content of iron， MDA and ROS in HSC‑T6 （P<0.05）. Sorafenib （5 μM， 10 μM， 20 μM） significantly inhibited the cell viability of PDGF‑BB‑enhanced HSC‑T6 （P<0.05）. When mTOR was inhibited， the protein expressions of α‑SMA， COL1α1 and GPX4 and the level of GSH were lower （P<0.05）， and the contents of iron， MDA and ROS were further increased （P<0.05）. When mTOR was activated， the results were reversed. Conclusion： Sorafenib induced ferroptosis to alleviate liver fibrosis， and inhibition of mTOR further enhanced the effect of sorafenib.
Objective： To investigate the role and mechanism of mTOR in sorafenib‑ameliorated liver fibrosis by inducing hepatic stellate cells （HSC） ferroptosis. Methods： The liver fibrosis models of C57BL/6 male mice were induced with carbon tetrachloride （CCl4） and randomly divided into normal group， model group， and sorafenib low （2.5 mg/kg）， medium （5 mg/kg）， and high （10 mg/kg） dose groups. Except for the normal group， the remaining four groups were treated with intraperitoneal injection of CCl4 for 8 weeks to establish liver fibrosis models. In addition， the corresponding concentrations of sorafenib were administered during the fourth week of modeling. Western blot was used to detect the expression of mTOR and p‑mTOR protein in liver tissues. In vitro experiments， HSC‑T6 cells were activated by PDGF‑BB and then treated with sorafenib （5 μM， 10 μM， 20 μM）， an mTOR inhibitor and activator. CCK8 was used to detect HSC‑T6 cell viability， Western blot detected the protein expression of α‑SMA， COL1α1， GPX4， mTOR and p‑mTOR. The levels of serum iron， GSH， and MDA were measured， and the intracellular ROS level was measured by 2'，7'‑dichlorofluorescein diacetate and dihydroethidium.Results： In vitro results showed that sorafenib significantly decreased the protein expression of α‑SMA， COL1α1， GPX4 and p‑mTOR， and decreased the level of GSH and increased the content of iron， MDA and ROS in HSC‑T6 （P<0.05）. Sorafenib （5 μM， 10 μM， 20 μM） significantly inhibited the cell viability of PDGF‑BB‑enhanced HSC‑T6 （P<0.05）. When mTOR was inhibited， the protein expressions of α‑SMA， COL1α1 and GPX4 and the level of GSH were lower （P<0.05）， and the contents of iron， MDA and ROS were further increased （P<0.05）. When mTOR was activated， the results were reversed. Conclusion： Sorafenib induced ferroptosis to alleviate liver fibrosis， and inhibition of mTOR further enhanced the effect of sorafenib.
2023(5):340-346, DOI: 10.13210/j.cnki.jhmu.20221229.003
Abstract:
Objective： To observe the effects of and explore the underlying mechanisms of Periplogenin （PPG） on the regulation of proliferation， apoptosis and migration of hepatocellular carcinoma Hep3B cell.Methods： Hep3B cells were divided into control group and PPG group. The Hep3B cells in the control group were not treated with PPG， and the other in the PPG group were intervened with 2.5， 5 and 12.5 µmol/mL PPG for 48 h. Cell viability was measured using the CCK⁃8 assay. Cell morphological changes was observed using by light microscope； The apoptotic state was observed by DAPI/PI staining. The colony⁃formation assay and scratch method was used for detecting the cell clone forming ability and cell migration ability， respectively. The cell apoptosis⁃related proteins Caspase⁃3， Cleaved Caspase⁃3， Bcl⁃2 and cell migration⁃related protein Matrix metalloproteinase⁃9 （MMP⁃9） were detected by Western Blot. Results： Compared with the control group， the cell proliferation level and the cell number in each PPG group were decreased， accompanying by cell volume reduction， chromatin pyknosis， and nuclear rupture in Hep3B cells. After PPG treatment， the clone formation level and cell mobility of Hep3B cells were decreased. Compared with the control group， the level of Bcl⁃2 protein was decreased， while the proportion of Cleaved Caspase⁃3/Caspase⁃3 protein was increased. The expression of MMP⁃9 proteins was decreased， with significantly statistically differences （P<0.05）.Conclusion： Periplogenin could inhibit the proliferation， and migration of Hep3B cells and also promote the cell apoptosis in a dose⁃dependent manner， which might be related to the activation of Caspase signaling pathway and inhibition of MMP⁃9 activity.
Objective： To observe the effects of and explore the underlying mechanisms of Periplogenin （PPG） on the regulation of proliferation， apoptosis and migration of hepatocellular carcinoma Hep3B cell.Methods： Hep3B cells were divided into control group and PPG group. The Hep3B cells in the control group were not treated with PPG， and the other in the PPG group were intervened with 2.5， 5 and 12.5 µmol/mL PPG for 48 h. Cell viability was measured using the CCK⁃8 assay. Cell morphological changes was observed using by light microscope； The apoptotic state was observed by DAPI/PI staining. The colony⁃formation assay and scratch method was used for detecting the cell clone forming ability and cell migration ability， respectively. The cell apoptosis⁃related proteins Caspase⁃3， Cleaved Caspase⁃3， Bcl⁃2 and cell migration⁃related protein Matrix metalloproteinase⁃9 （MMP⁃9） were detected by Western Blot. Results： Compared with the control group， the cell proliferation level and the cell number in each PPG group were decreased， accompanying by cell volume reduction， chromatin pyknosis， and nuclear rupture in Hep3B cells. After PPG treatment， the clone formation level and cell mobility of Hep3B cells were decreased. Compared with the control group， the level of Bcl⁃2 protein was decreased， while the proportion of Cleaved Caspase⁃3/Caspase⁃3 protein was increased. The expression of MMP⁃9 proteins was decreased， with significantly statistically differences （P<0.05）.Conclusion： Periplogenin could inhibit the proliferation， and migration of Hep3B cells and also promote the cell apoptosis in a dose⁃dependent manner， which might be related to the activation of Caspase signaling pathway and inhibition of MMP⁃9 activity.
2023(5):347-351, DOI: 10.13210/j.cnki.jhmu.20230117.001
Abstract:
Objective： To investigate the effect of Wujiadan Recipe on PLC‑β1， IP3， IP3R， Cam， PLC‑β1， IP3R1 mRNA and GnRH， Cam protein from hypothalamus tissue of ovariectomized female rats， and to further clarify whether Wujiadan Recipe plays a role through PLC‑β/IP3/CaM signal pathway.Methods： We randomly divided 64 SD adult female rats into NG group（normal group）， MG group（the model group）， TMC group（the control group with traditional Chinese medicine ），WMC group（the control group with western medicine ） with 10 rats in each， and Wujiadan Recipe high（WH）， Wujiadan Recipe medium（WM） and Wujiadan Recipe low dose group（WL） with 8 rats in each. Except the NG group， we removed the ovarian tissues of other six groups to establish the ovariectomized model. When the model is successfully replicated， we give the medicine to the rats in each group once a day. The TMC and WMC group were given the corresponding dose of Gengnian'an tablet suspension and Bujiale suspension by gavage respectively. The NG and MG group were given equal measure of normal saline. After 4 weeks， the rats were killed. We use ELISA to detecte the levels of PLC‑β1， IP3， IP3R and Cam ； and we use PCR to detecte the mRNA expression levels of PLC‑βand IP3R1； and use WesternBlot to detecte the protein expression levels of Cam and GnRH in rat hypothalamus tissue. Results： The test results of PLC‑β1， IP3， IP3R and Cam in the hypothalamus homogenate of the model group were significantly increased than the NG group （P<0.01）. The results of PLC‑β1 and IP3R1 and GnRHmRNA were significantly increased （P<0.01）， and the expressions of GnRH and Cam protein were higher than NG （P<0.01）. Wujiadan Recipe could reduce the results of PLC‑β1， IP3， IP3R and Cam in hypothalamus of ovariectomized rats， and reduce the expression of PLC‑β1 and IP3R1 and GnRH mRNA. Conclusion： Wujiadan Recipe down‑regulates GnRH expression， which may be caused by acting on PLC‑β1， IP3， IP3R， CaM in hypothalamus of ovariectomized rats， so as to coordinate the function of reproductive axis and improve the reproductive endocrine level of patients with menopausal syndrome.
Objective： To investigate the effect of Wujiadan Recipe on PLC‑β1， IP3， IP3R， Cam， PLC‑β1， IP3R1 mRNA and GnRH， Cam protein from hypothalamus tissue of ovariectomized female rats， and to further clarify whether Wujiadan Recipe plays a role through PLC‑β/IP3/CaM signal pathway.Methods： We randomly divided 64 SD adult female rats into NG group（normal group）， MG group（the model group）， TMC group（the control group with traditional Chinese medicine ），WMC group（the control group with western medicine ） with 10 rats in each， and Wujiadan Recipe high（WH）， Wujiadan Recipe medium（WM） and Wujiadan Recipe low dose group（WL） with 8 rats in each. Except the NG group， we removed the ovarian tissues of other six groups to establish the ovariectomized model. When the model is successfully replicated， we give the medicine to the rats in each group once a day. The TMC and WMC group were given the corresponding dose of Gengnian'an tablet suspension and Bujiale suspension by gavage respectively. The NG and MG group were given equal measure of normal saline. After 4 weeks， the rats were killed. We use ELISA to detecte the levels of PLC‑β1， IP3， IP3R and Cam ； and we use PCR to detecte the mRNA expression levels of PLC‑βand IP3R1； and use WesternBlot to detecte the protein expression levels of Cam and GnRH in rat hypothalamus tissue. Results： The test results of PLC‑β1， IP3， IP3R and Cam in the hypothalamus homogenate of the model group were significantly increased than the NG group （P<0.01）. The results of PLC‑β1 and IP3R1 and GnRHmRNA were significantly increased （P<0.01）， and the expressions of GnRH and Cam protein were higher than NG （P<0.01）. Wujiadan Recipe could reduce the results of PLC‑β1， IP3， IP3R and Cam in hypothalamus of ovariectomized rats， and reduce the expression of PLC‑β1 and IP3R1 and GnRH mRNA. Conclusion： Wujiadan Recipe down‑regulates GnRH expression， which may be caused by acting on PLC‑β1， IP3， IP3R， CaM in hypothalamus of ovariectomized rats， so as to coordinate the function of reproductive axis and improve the reproductive endocrine level of patients with menopausal syndrome.
2023(5):352-356, DOI: 10.13210/j.cnki.jhmu.20230103.001
Abstract:
Objective： To observe the effect of electroacupuncture （EA） at "Baihui" （GV20） and "Shenshu" （BL23） on cognitive impairment in AD model mice， and to explore its mechanism. Methods： A total of 24 model mice were randomly divided into EA， medication and model groups， 8 mice in each group. Another 8 C57BL/6J mice were used as the normal control. The rats in the EA group were treated with electrical stimulation at Baihui （GV 20） and Shenshu （BL 23）， and those in the medication group were treated with donepezil hydrochloride， once a day for 21 days. Adopting the Maorris water maze method to detect the behavior of mice and using HE staining to observe the morphological structure of neurons in the hippocampal region of mice. Finally the expression of GSK‑3β and β‑catenin protein contents in the hippocampus of mice in each group was detected by Western blot. Results： Compared to the model group， the evasion latency of the electroacupuncture group and the western medicine group were significantly shorter， and the dwell time in the target quadrant and the number of crossing the plateau were increased （P<0.05）， and the hippocampal neurons in each treatment group were closely arranged and complete， with a clearer hierarchy. Western blot assay results showed that the expression of β‑catenin protein was significantly increased and GSK‑3β protein expression was decreased in the hippocampal region of mice in the electroacupuncture and western medicine groups compared with the model group （P < 0.05）.Conclusion： Ea at "Baihui" （GV20） and "Shenshu" （BL23） can significantly improve the cognitive function of APP/PS1 mice， which may be connected with the activation of Wnt/β‑catenin signaling pathway.
Objective： To observe the effect of electroacupuncture （EA） at "Baihui" （GV20） and "Shenshu" （BL23） on cognitive impairment in AD model mice， and to explore its mechanism. Methods： A total of 24 model mice were randomly divided into EA， medication and model groups， 8 mice in each group. Another 8 C57BL/6J mice were used as the normal control. The rats in the EA group were treated with electrical stimulation at Baihui （GV 20） and Shenshu （BL 23）， and those in the medication group were treated with donepezil hydrochloride， once a day for 21 days. Adopting the Maorris water maze method to detect the behavior of mice and using HE staining to observe the morphological structure of neurons in the hippocampal region of mice. Finally the expression of GSK‑3β and β‑catenin protein contents in the hippocampus of mice in each group was detected by Western blot. Results： Compared to the model group， the evasion latency of the electroacupuncture group and the western medicine group were significantly shorter， and the dwell time in the target quadrant and the number of crossing the plateau were increased （P<0.05）， and the hippocampal neurons in each treatment group were closely arranged and complete， with a clearer hierarchy. Western blot assay results showed that the expression of β‑catenin protein was significantly increased and GSK‑3β protein expression was decreased in the hippocampal region of mice in the electroacupuncture and western medicine groups compared with the model group （P < 0.05）.Conclusion： Ea at "Baihui" （GV20） and "Shenshu" （BL23） can significantly improve the cognitive function of APP/PS1 mice， which may be connected with the activation of Wnt/β‑catenin signaling pathway.
2023(5):357-365, DOI: 10.13210/j.cnki.jhmu.20210520.002
Abstract:
Objective： To explore the potential active components， therapeutic targets and critical path of Alpinia officinarum and Pogostemonis Herba in the treatment of diabetic gastroparesis （DGP） by using network pharmacology.Methods： The main chemical components and corresponding targets genes of A. officinarum‑ Pogostemonis Herba were screened through TCMSP database retrieval ［oral bioavailability（OB）≥30% and drug like（DL）≥0.18］. Tgenes of diabetic gastroparesis were screened by the Human Gene Database （GeneCards）， and Venny 2.1 software was used to obtained common targets for the active ingredients of A. officinarum‑ Pogostemonis Herba and DGP. Then， the protein‑protein interaction （PPI） network of the common targets was constructed by STRING database and analyzed to performed the core targets. GO function and KEGG pathway enrichment analysis of the common target genes were obtained by using ClusterProfiler R package. Finally， the network diagram of "active ingredient ‑pathway ‑target" was used to establish by Cytoscape3.8 software. Results： Totally 23 ingredients of A. officinarum‑Pogostemonis Herba， 97 active ingredients targets and 533 DGP related targets， including 46 common targets were selected. The common targets were mainly enriched in the cell constituents such as the nuclear chromatin and mitochondria outer membrane，involved in the biological processes as oxidative stress， apoptosis signal regulation， and molecular functions as enzyme binding， protein phosphatase binding， and cytokine activity. They were also concentrated in the signal pathways such as PI3K/Akt，HIF‑ 1 and MAPK. The network of“active ingredients‑ targets‑ pathways”indicated the active components such as quercetin，kaempferol and galangin in A. officinarum‑ Pogostemonis Herba played an anti‑ delayed gastric emptying in diabetic gastroparesis by acting on PTGS2， NOS2， BCL2， IL6， VEGFA and other targets to jointly regulate PI3K‑ Akt， HIF‑ 1 and MAPK pathways.Conclusion： This study initially reveals that the combined treatment of A. officinarum‑Pogostemonis Herbafor delayed gastric emptying in diabetic gastroparesis is a complex process with multi‑components， multi‑targets and multi‑pathways， and provides a new idea for follow‑up researches.
Objective： To explore the potential active components， therapeutic targets and critical path of Alpinia officinarum and Pogostemonis Herba in the treatment of diabetic gastroparesis （DGP） by using network pharmacology.Methods： The main chemical components and corresponding targets genes of A. officinarum‑ Pogostemonis Herba were screened through TCMSP database retrieval ［oral bioavailability（OB）≥30% and drug like（DL）≥0.18］. Tgenes of diabetic gastroparesis were screened by the Human Gene Database （GeneCards）， and Venny 2.1 software was used to obtained common targets for the active ingredients of A. officinarum‑ Pogostemonis Herba and DGP. Then， the protein‑protein interaction （PPI） network of the common targets was constructed by STRING database and analyzed to performed the core targets. GO function and KEGG pathway enrichment analysis of the common target genes were obtained by using ClusterProfiler R package. Finally， the network diagram of "active ingredient ‑pathway ‑target" was used to establish by Cytoscape3.8 software. Results： Totally 23 ingredients of A. officinarum‑Pogostemonis Herba， 97 active ingredients targets and 533 DGP related targets， including 46 common targets were selected. The common targets were mainly enriched in the cell constituents such as the nuclear chromatin and mitochondria outer membrane，involved in the biological processes as oxidative stress， apoptosis signal regulation， and molecular functions as enzyme binding， protein phosphatase binding， and cytokine activity. They were also concentrated in the signal pathways such as PI3K/Akt，HIF‑ 1 and MAPK. The network of“active ingredients‑ targets‑ pathways”indicated the active components such as quercetin，kaempferol and galangin in A. officinarum‑ Pogostemonis Herba played an anti‑ delayed gastric emptying in diabetic gastroparesis by acting on PTGS2， NOS2， BCL2， IL6， VEGFA and other targets to jointly regulate PI3K‑ Akt， HIF‑ 1 and MAPK pathways.Conclusion： This study initially reveals that the combined treatment of A. officinarum‑Pogostemonis Herbafor delayed gastric emptying in diabetic gastroparesis is a complex process with multi‑components， multi‑targets and multi‑pathways， and provides a new idea for follow‑up researches.
2023(5):366-373, DOI: 10.13210/j.cnki.jhmu.20230131.002
Abstract:
Objective： To identify the prevalence and influencing factors of allergic rhinitis among middle school students in Haikou， and to provide reference for the prevention and treatment of allergic rhinitis among middle school students in Haikou.Methods： The stratified cluster sampling method was adopted to randomly select the middle school students （aged 12‑18） from 8 ordinary or vocational middle schools in Xiuying District， Longhua District， Meilan District and Qiongshan District of Haikou as the survey subjects. The self‑made questionnaire was used to conduct the survey from April to May in 2022.Results： A total of 2 169 valid questionnaires were collected， the effective rate was 86.6% （2 169/2 479）， 814 （37.5%） male students and 1 355 （62.5%） female students. The prevalence rate of allergic rhinitis reported by middle school students in Haikou was 39.8% （863/2 169）， including 599 cases of intermittent allergic rhinitis （69.4%） and 264 cases of persistent allergic rhinitis （30.6%）. The January， February， November and December were the periods of high incidence of symptoms each year. The results of multivariate logistic regression analysis showed that： using antibiotics ≥5 times a year ［OR=2.07， 95%CI （1.24‑3.45），P=0.005］， using antibiotics 3~4 times a year ［OR=1.77， 95%CI （1.23‑2.55）， P=0.002］， family history of allergic rhinitis ［OR=3.84， 95%CI（2.67‑5.54），P< 0.001］， history of sinusitis ［OR=7.77， 95%CI（3.22‑18.76）， P< 0.001］， damp living environment ［OR=2.87， 95%CI（2.00‑4.13）， P < 0.001］， history of asthma ［OR=8.69， 95%CI（1.93‑39.12）， P=0.005］， urban residents ［OR=1.68， 95%CI （1.35‑2.09），P<0.001］， frequent exposure to dust ［OR=1.59， 95%CI（1.20‑2.12），P=0.001］， male ［OR=1.35， 95%CI（1.10‑1.66），P=0.005］， furry pet ［OR=1.39， 95%CI（1.10‑1.75），P=0.006］， daily sleep time < 8 hours ［OR=1.30， 95%CI （1.07‑1.58），P=0.009］were risk factors for allergic rhinitis.Conclusion： The prevalence of allergic rhinitis among middle school students in Haikou was higher than reported in most areas of China. Male， urban residents， antibiotic use， sleeping less than 8 hours a day， feeding furry pets， living in humid environment， frequent exposure to dust， family history of allergic rhinitis， history of sinusitis and history of asthma were risk factors for allergic rhinitis. Family history of allergic rhinitis， sinusitis and asthma were the most significant factors.
Objective： To identify the prevalence and influencing factors of allergic rhinitis among middle school students in Haikou， and to provide reference for the prevention and treatment of allergic rhinitis among middle school students in Haikou.Methods： The stratified cluster sampling method was adopted to randomly select the middle school students （aged 12‑18） from 8 ordinary or vocational middle schools in Xiuying District， Longhua District， Meilan District and Qiongshan District of Haikou as the survey subjects. The self‑made questionnaire was used to conduct the survey from April to May in 2022.Results： A total of 2 169 valid questionnaires were collected， the effective rate was 86.6% （2 169/2 479）， 814 （37.5%） male students and 1 355 （62.5%） female students. The prevalence rate of allergic rhinitis reported by middle school students in Haikou was 39.8% （863/2 169）， including 599 cases of intermittent allergic rhinitis （69.4%） and 264 cases of persistent allergic rhinitis （30.6%）. The January， February， November and December were the periods of high incidence of symptoms each year. The results of multivariate logistic regression analysis showed that： using antibiotics ≥5 times a year ［OR=2.07， 95%CI （1.24‑3.45），P=0.005］， using antibiotics 3~4 times a year ［OR=1.77， 95%CI （1.23‑2.55）， P=0.002］， family history of allergic rhinitis ［OR=3.84， 95%CI（2.67‑5.54），P< 0.001］， history of sinusitis ［OR=7.77， 95%CI（3.22‑18.76）， P< 0.001］， damp living environment ［OR=2.87， 95%CI（2.00‑4.13）， P < 0.001］， history of asthma ［OR=8.69， 95%CI（1.93‑39.12）， P=0.005］， urban residents ［OR=1.68， 95%CI （1.35‑2.09），P<0.001］， frequent exposure to dust ［OR=1.59， 95%CI（1.20‑2.12），P=0.001］， male ［OR=1.35， 95%CI（1.10‑1.66），P=0.005］， furry pet ［OR=1.39， 95%CI（1.10‑1.75），P=0.006］， daily sleep time < 8 hours ［OR=1.30， 95%CI （1.07‑1.58），P=0.009］were risk factors for allergic rhinitis.Conclusion： The prevalence of allergic rhinitis among middle school students in Haikou was higher than reported in most areas of China. Male， urban residents， antibiotic use， sleeping less than 8 hours a day， feeding furry pets， living in humid environment， frequent exposure to dust， family history of allergic rhinitis， history of sinusitis and history of asthma were risk factors for allergic rhinitis. Family history of allergic rhinitis， sinusitis and asthma were the most significant factors.
2023(5):374-381, DOI: 10.13210/j.cnki.jhmu.20221221.003
Abstract:
Objective： To explore relationships between CANX expression， prognosis and immune infiltration of acute myeloid leukemia （AML） patients.Methods： The TIMER database was used to compare the CANX expression among a variety of TCGA tumor and normal tissue samples. The difference of CANX expression between AML and normal samples was found by R software. The Kaplan‑Meier method and Log‑Rank test were used to assess the relationship between CANX expression and patient survival. The R software was used to find correlations between CANX expression， clinical characteristics， drug sensitivity， and immune infiltration in AML.Results： The differential expression of CANX in 13 tumor and normal tissue samples were statistically significant （P<0.05）. The high CANX expression was associated with a favorable prognosis （P<0.05）， which was validated in GSE37642. The expression of CANX was correlated with age， survival status， FAB stage， and karyotype （P<0.05）. High CANX expression， low age and favorable karyotype were independent predictors of a favorable prognosis （P<0.05）. CANX expression may affect the sensitivity of AML patients to multiple drugs （P<0.05）. The expression of CANX was positively correlated with that of M1 macrophages and CD8 T cells.Conclusion： CANX may be used as a novel potential biomarker， and could benefit AML patients by predicting patient prognosis and providing precise treatment indications.
Objective： To explore relationships between CANX expression， prognosis and immune infiltration of acute myeloid leukemia （AML） patients.Methods： The TIMER database was used to compare the CANX expression among a variety of TCGA tumor and normal tissue samples. The difference of CANX expression between AML and normal samples was found by R software. The Kaplan‑Meier method and Log‑Rank test were used to assess the relationship between CANX expression and patient survival. The R software was used to find correlations between CANX expression， clinical characteristics， drug sensitivity， and immune infiltration in AML.Results： The differential expression of CANX in 13 tumor and normal tissue samples were statistically significant （P<0.05）. The high CANX expression was associated with a favorable prognosis （P<0.05）， which was validated in GSE37642. The expression of CANX was correlated with age， survival status， FAB stage， and karyotype （P<0.05）. High CANX expression， low age and favorable karyotype were independent predictors of a favorable prognosis （P<0.05）. CANX expression may affect the sensitivity of AML patients to multiple drugs （P<0.05）. The expression of CANX was positively correlated with that of M1 macrophages and CD8 T cells.Conclusion： CANX may be used as a novel potential biomarker， and could benefit AML patients by predicting patient prognosis and providing precise treatment indications.
2023(5):382-389, DOI: 10.13210/j.cnki.jhmu.20210527.001
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Objective： To evaluate the prognostic value of platelet‑to‑lymphocyte ratio （PLR） in upper tract urothelial carcinoma （UTUC） patients treated with radical nephroureterectomy. Methods： This study was pre‑registered in PROSPERO （No. CRD42020186645）. Two reviewers independently performed a comprehensive literature search on PubMed， Web of Science， EMBASE， Cochrane Library databases， China Biology Medicine disc （CBM）， Wanfang database， and China National Knowledge Infrastructure （CNKI） from inception to May 2021 for prospective or retrospective cohort studies evaluating the prognostic value of PLR. Literature was analyzed with inclusion and exclusion criteria. Data and other useful clinicopathological features were extracted and analyzed with fixed or random‑effect models when applicable. Heterogeneity was assessed on the basis of Cochran’s Q test and I2 statistic. Publication bias was assessed with funnel plots and precisely assessed by Egger's tests. Results： A total of ten studies involving 3 287 UTUC patients were included. Meta‑analysis showed that elevated preoperative PLR was associated with worse overall survival （HR=1.51， 95%P 1.17~1.94， CI=0.001）， cancer specific survival （HR=1.52， 95%CI 1.21~1.90，P<0.001）， disease free survival （HR=1.32， 95% CI 1.12~1.56，P=0.001）， and progression free survival （HR=1.88， 95% CI 1.41~2.52， P<0.001）. Furthermore， the sensitivity analyses validated the stability and reliability of the results.Conclusion： The present meta‑analysis demonstrates a significant association between elevated preoperative PLR and poor prognosis of UTUC patients treated with radical nephroureterectomy. Hence， PLR could be helpful as a potential prognostic biomarker to guide clinical decision‑making and select individualized treatment strategies for UTUC patients. However， more prospective and large‑scale trials are needed to provide more evidence.
Objective： To evaluate the prognostic value of platelet‑to‑lymphocyte ratio （PLR） in upper tract urothelial carcinoma （UTUC） patients treated with radical nephroureterectomy. Methods： This study was pre‑registered in PROSPERO （No. CRD42020186645）. Two reviewers independently performed a comprehensive literature search on PubMed， Web of Science， EMBASE， Cochrane Library databases， China Biology Medicine disc （CBM）， Wanfang database， and China National Knowledge Infrastructure （CNKI） from inception to May 2021 for prospective or retrospective cohort studies evaluating the prognostic value of PLR. Literature was analyzed with inclusion and exclusion criteria. Data and other useful clinicopathological features were extracted and analyzed with fixed or random‑effect models when applicable. Heterogeneity was assessed on the basis of Cochran’s Q test and I2 statistic. Publication bias was assessed with funnel plots and precisely assessed by Egger's tests. Results： A total of ten studies involving 3 287 UTUC patients were included. Meta‑analysis showed that elevated preoperative PLR was associated with worse overall survival （HR=1.51， 95%P 1.17~1.94， CI=0.001）， cancer specific survival （HR=1.52， 95%CI 1.21~1.90，P<0.001）， disease free survival （HR=1.32， 95% CI 1.12~1.56，P=0.001）， and progression free survival （HR=1.88， 95% CI 1.41~2.52， P<0.001）. Furthermore， the sensitivity analyses validated the stability and reliability of the results.Conclusion： The present meta‑analysis demonstrates a significant association between elevated preoperative PLR and poor prognosis of UTUC patients treated with radical nephroureterectomy. Hence， PLR could be helpful as a potential prognostic biomarker to guide clinical decision‑making and select individualized treatment strategies for UTUC patients. However， more prospective and large‑scale trials are needed to provide more evidence.
2023(5):390-394, DOI: 10.13210/j.cnki.jhmu.20210818.001
Abstract:
At present， ischemic stroke seriously affects people's life and health， and its occurrence， development and therapeutic effect are affected by many factors. With the deep research on ischemic cerebral apoplexy disease， people have a deeper understanding of its virulence genes. The apolipoprotein E genotype is the research focus recently， its genetic type is not only involved in the occurrence and development of ischemic cerebral apoplexy， but also causes different therapeatic effects. In this paper， we reviewed the relationship between apolipoprotein E gene polymorphism and lipid metabolism and atherosclerosis in ischemic stroke， as well as the differences in the therapeutic effects of thrombolysis， thrombectomy and lipid‑lowering among different genotypes.
At present， ischemic stroke seriously affects people's life and health， and its occurrence， development and therapeutic effect are affected by many factors. With the deep research on ischemic cerebral apoplexy disease， people have a deeper understanding of its virulence genes. The apolipoprotein E genotype is the research focus recently， its genetic type is not only involved in the occurrence and development of ischemic cerebral apoplexy， but also causes different therapeatic effects. In this paper， we reviewed the relationship between apolipoprotein E gene polymorphism and lipid metabolism and atherosclerosis in ischemic stroke， as well as the differences in the therapeutic effects of thrombolysis， thrombectomy and lipid‑lowering among different genotypes.
2023(5):395-400, DOI: 10.13210/j.cnki.jhmu.20220506.002
Abstract:
With the influence of many factors such as the aging of the population， the younger smokers， and the serious air pollution， the incidence of chronic respiratory diseases is increasing year by year. In the treatment of respiratory diseases， clinical intervention is still mainly based on drug control of pulmonary symptoms. However， systemic drugs have disadvantages such as many adverse reactions and severe systemic side effects. In recent years， the research and development of local drug delivery systems for the respiratory tract has brought new changes to the treatment of respiratory diseases. Locally delivered drugs can directly act on the airways and have the characteristics of fast onset， good curative effect and small side effects. It is a simple， efficient and safe treatment method， which has a very significant effect， and has become a hot topic of current research and promotion. This paper briefly reviews the development track and latest research progress of respiratory local drug delivery systems at home and abroad， in order to provide reference for clinical workers in drug selection and application.
With the influence of many factors such as the aging of the population， the younger smokers， and the serious air pollution， the incidence of chronic respiratory diseases is increasing year by year. In the treatment of respiratory diseases， clinical intervention is still mainly based on drug control of pulmonary symptoms. However， systemic drugs have disadvantages such as many adverse reactions and severe systemic side effects. In recent years， the research and development of local drug delivery systems for the respiratory tract has brought new changes to the treatment of respiratory diseases. Locally delivered drugs can directly act on the airways and have the characteristics of fast onset， good curative effect and small side effects. It is a simple， efficient and safe treatment method， which has a very significant effect， and has become a hot topic of current research and promotion. This paper briefly reviews the development track and latest research progress of respiratory local drug delivery systems at home and abroad， in order to provide reference for clinical workers in drug selection and application.
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Abstract:
Objective: To study the effect of Baihe Dihuang Decoction in improving anxiety and its mechanism of action. Methods: Male mice were randomly divided into 5 groups: blank control group, Baihe Dihuang Decoction high, medium, low-dose group and diazepam group. After continuous intragastric administration for 14 d, the behavioral test of the mouse elevated plus maze experiment and opening test was performed. The brain tissue GABA and Glu content was measured by immunosorbent assay (ELISA). Results: Compared with the blank control group, the middle and high doses of Baihe Dihuang Decoction could increase the time and frequency of movement of the mice in the open cross labyrinth in the open arm and increase the number of times that the mouse entered the central area in the opening test. The anti-anxiety effect also showed an increase in the brain tissue GABA content in mice, ignificantly decreased Glu contents in mice. Conclusion: Baihe Dihuang Decoction has some anxiolytic effect, and antianxiety effect may be related to increasing brain tissue GABA content, decreased Glu contents in mice.
Objective: To study the effect of Baihe Dihuang Decoction in improving anxiety and its mechanism of action. Methods: Male mice were randomly divided into 5 groups: blank control group, Baihe Dihuang Decoction high, medium, low-dose group and diazepam group. After continuous intragastric administration for 14 d, the behavioral test of the mouse elevated plus maze experiment and opening test was performed. The brain tissue GABA and Glu content was measured by immunosorbent assay (ELISA). Results: Compared with the blank control group, the middle and high doses of Baihe Dihuang Decoction could increase the time and frequency of movement of the mice in the open cross labyrinth in the open arm and increase the number of times that the mouse entered the central area in the opening test. The anti-anxiety effect also showed an increase in the brain tissue GABA content in mice, ignificantly decreased Glu contents in mice. Conclusion: Baihe Dihuang Decoction has some anxiolytic effect, and antianxiety effect may be related to increasing brain tissue GABA content, decreased Glu contents in mice.
Abstract:
Objective: The current study aims to identify the effects of exogenous application of pulsed electromagnetic fields on skin wound healing in diabetic rats, and thus provides experimental evidence for its more scientific clinical application in the future. Methods: The diabetic animal models were established via intraperitoneal injection of streptozotocin in 4-monthold male rats. Fourth eight rats were randomly assigned into the diabetes group (DM) and diabetes coupled with pulsed electromagnetic fields (DM+PEMF) group. Another 24 normal rats were used as the blank control group (Control). Then, all the rats in the three groups were subjected to dorsal surgery for the establishment of soft tissue wound model (circular wound with the diameter of 2 cm). Then, the rats in the DM+PEMF group were subjected to pulsed electromagnetic fields stimulation. Then, 6 rats in each group were sacrificed at Day 5, 12 and 19 post surgery. The glucose levels, wound closure, wound healing time and tissue tensile strength were examined and analyzed. Results: Pulsed electromagnetic fields significantly increased the wound closure rate in diabetic rats at Day 5, 12 and 19 post surgery, decreased overall wound healing period in diabetic rats, and also enhanced tissue tensile strength in diabetic rats at Day 5, 12 and 19 post surgery. Conclusion: Low-intensity pulsed electromagnetic fields can significantly accelerate diabetic wound healing process, and also improve diabetic tissue repair capacity. This study may be helpful for providing more scientific and reasonable experimental evidence for the treatment of pulsed electromagnetic fields on diabetic wound healing in clinics.
Objective: The current study aims to identify the effects of exogenous application of pulsed electromagnetic fields on skin wound healing in diabetic rats, and thus provides experimental evidence for its more scientific clinical application in the future. Methods: The diabetic animal models were established via intraperitoneal injection of streptozotocin in 4-monthold male rats. Fourth eight rats were randomly assigned into the diabetes group (DM) and diabetes coupled with pulsed electromagnetic fields (DM+PEMF) group. Another 24 normal rats were used as the blank control group (Control). Then, all the rats in the three groups were subjected to dorsal surgery for the establishment of soft tissue wound model (circular wound with the diameter of 2 cm). Then, the rats in the DM+PEMF group were subjected to pulsed electromagnetic fields stimulation. Then, 6 rats in each group were sacrificed at Day 5, 12 and 19 post surgery. The glucose levels, wound closure, wound healing time and tissue tensile strength were examined and analyzed. Results: Pulsed electromagnetic fields significantly increased the wound closure rate in diabetic rats at Day 5, 12 and 19 post surgery, decreased overall wound healing period in diabetic rats, and also enhanced tissue tensile strength in diabetic rats at Day 5, 12 and 19 post surgery. Conclusion: Low-intensity pulsed electromagnetic fields can significantly accelerate diabetic wound healing process, and also improve diabetic tissue repair capacity. This study may be helpful for providing more scientific and reasonable experimental evidence for the treatment of pulsed electromagnetic fields on diabetic wound healing in clinics.
Abstract:
Gastric cancer is one of common malignant tumors in clinic. It has high incidence, high mortality and poor prognosis. Generally, gastric cancer is divided into early gastric cancer (EGC) and advanced gastric cancer. The 5-year survival rate of EGC is higher than that of advanced gastric cancer. In recent years, with the continuous development of science and technology, a variety of medical instruments and technologies have been updated, greatly promoting the detection rate of EGC, reducing the rate of EGC missed diagnosis. This article reviews the progress of EGC endoscopic diagnosis.
Gastric cancer is one of common malignant tumors in clinic. It has high incidence, high mortality and poor prognosis. Generally, gastric cancer is divided into early gastric cancer (EGC) and advanced gastric cancer. The 5-year survival rate of EGC is higher than that of advanced gastric cancer. In recent years, with the continuous development of science and technology, a variety of medical instruments and technologies have been updated, greatly promoting the detection rate of EGC, reducing the rate of EGC missed diagnosis. This article reviews the progress of EGC endoscopic diagnosis.
Abstract:
Objective: To investigate the effects of routine rehabilitation therapy combined with low frequency head stimulation on monoamine neurotransmitters, neuron-specific enolase (NSE), endothelin-1 (ET-1) and cerebral hemodynamics in children with cerebral palsy. Methods: From January 2017 to June 2018, 110 children with cerebral palsy were randomly divided into observation group (55 cases) and control group (55 cases). The control group received routine rehabilitation treatment, while the observation group received low-frequency head stimulation on the basis of routine rehabilitation treatment. The changes of dopamine (DA), norepinephrine (NE), serotonin (5-HT), NSE, ET-1 levels and mean blood flow velocity of anterior cerebral artery (ACA), middle cerebral artery (MCA), posterior cerebral artery (PCA) were compared in two groups. Results: Before treatment, there was no significant difference in DA, NE and 5-HT levels in two groups. After treatment, DA, NE and 5-HT levels in the observation group were (192.23±22.71) ng/mL, (98.02±11.71) ng/L, (210.07±25.03) ng/L, and in the control group. the DA, NE, 5-HT levels were (147.06±17.02) ng/mL, (83.07±11.15) ng/L, and (171.88±20.45) ng/L, respectively. The DA, NE and 5-HT levels in two groups were higher than those before treatment, and DA, NE and 5-HT levels in the observation group were higher than those in the control group. Before treatment, there was no significant difference in NSE and ET-1 levels between the two groups. After treatment, the NSE and ET-1 levels in the observation group were (7.97±2.07) μg/L and (41.01±10.07) pg/ mL, and the NSE and ET-1 levels in the control group were (10.38±3.02) μg/L, (58.46±15.02) pg/ mL, respectively. the NSE and ET-1 in two groups were lower than those before treatment, and the NSE and ET-1 of the observation group were lower than the control group. Before treatment, there was no significant difference in mean blood flow velocity between ACA, MCA and PCA. After treatment, the mean blood flow velocities of ACA, MCA, and PCA in the observation group were (46.88±7.72) cm/s, (59.85±10.18) cm/s, and (49.15±7.02) cm/s, respectively, which was significantly higher than before treatment and higher than that of the control group in the same period. Conclusion: Conventional rehabilitation combined with low-frequency electrical stimulation of the head can effectively increase the content of monoamine neurotransmitters in children with cerebral palsy, enhance cerebral blood circulation, and reduce brain damage
Objective: To investigate the effects of routine rehabilitation therapy combined with low frequency head stimulation on monoamine neurotransmitters, neuron-specific enolase (NSE), endothelin-1 (ET-1) and cerebral hemodynamics in children with cerebral palsy. Methods: From January 2017 to June 2018, 110 children with cerebral palsy were randomly divided into observation group (55 cases) and control group (55 cases). The control group received routine rehabilitation treatment, while the observation group received low-frequency head stimulation on the basis of routine rehabilitation treatment. The changes of dopamine (DA), norepinephrine (NE), serotonin (5-HT), NSE, ET-1 levels and mean blood flow velocity of anterior cerebral artery (ACA), middle cerebral artery (MCA), posterior cerebral artery (PCA) were compared in two groups. Results: Before treatment, there was no significant difference in DA, NE and 5-HT levels in two groups. After treatment, DA, NE and 5-HT levels in the observation group were (192.23±22.71) ng/mL, (98.02±11.71) ng/L, (210.07±25.03) ng/L, and in the control group. the DA, NE, 5-HT levels were (147.06±17.02) ng/mL, (83.07±11.15) ng/L, and (171.88±20.45) ng/L, respectively. The DA, NE and 5-HT levels in two groups were higher than those before treatment, and DA, NE and 5-HT levels in the observation group were higher than those in the control group. Before treatment, there was no significant difference in NSE and ET-1 levels between the two groups. After treatment, the NSE and ET-1 levels in the observation group were (7.97±2.07) μg/L and (41.01±10.07) pg/ mL, and the NSE and ET-1 levels in the control group were (10.38±3.02) μg/L, (58.46±15.02) pg/ mL, respectively. the NSE and ET-1 in two groups were lower than those before treatment, and the NSE and ET-1 of the observation group were lower than the control group. Before treatment, there was no significant difference in mean blood flow velocity between ACA, MCA and PCA. After treatment, the mean blood flow velocities of ACA, MCA, and PCA in the observation group were (46.88±7.72) cm/s, (59.85±10.18) cm/s, and (49.15±7.02) cm/s, respectively, which was significantly higher than before treatment and higher than that of the control group in the same period. Conclusion: Conventional rehabilitation combined with low-frequency electrical stimulation of the head can effectively increase the content of monoamine neurotransmitters in children with cerebral palsy, enhance cerebral blood circulation, and reduce brain damage
Abstract:
Objective: To study the refractory factors associated with schizophrenia. Methods: 200 patients with refractory schizophrenia and 200 patients with non-refractory schizophrenia were selected. The CYP series of genes CYP1A2, CYP3A4 and CYP2D6 were detected by drug gene, and the rapid metabolic probabilities of the three genes were compared and analyzed. 200 patients with refractory schizophrenia were randomly divided into two groups: the combined drug treatment group and the single drug treatment group. The results were compared between the treatment of 0W and 4W for drug gene detection, 3 genes fast metabolizing type and BPRS scale. analysis. Results: The rapid metabolizing probability and non-refractory difference of CYP1A2, CYP3A4 and CYP2D6 genes in patients with refractory schizophrenia were significant. The comparison of fast metabotropic probabilities of CYP1A2, CYP3A4 and CYP2D6 genes in patients treated with 4W after treatment There was no significant difference in the single drug treatment group. The BPRS scale score was significantly higher in the drug-treated group than in the single-drug group. After logistic regression analysis, the refractory characteristics of schizophrenia and The CYP series of genes CYP1A2, CYP3A4, and CYP2D6 are rapidly metabolized. Conclusion: CYP series of genes CYP1A2, CYP3A4, CYP2D6 fast metabolites are related factors of refractory schizophrenia, antipsychotic drugs combined with CYP enzyme inhibitor treatment can improve the efficacy.
Objective: To study the refractory factors associated with schizophrenia. Methods: 200 patients with refractory schizophrenia and 200 patients with non-refractory schizophrenia were selected. The CYP series of genes CYP1A2, CYP3A4 and CYP2D6 were detected by drug gene, and the rapid metabolic probabilities of the three genes were compared and analyzed. 200 patients with refractory schizophrenia were randomly divided into two groups: the combined drug treatment group and the single drug treatment group. The results were compared between the treatment of 0W and 4W for drug gene detection, 3 genes fast metabolizing type and BPRS scale. analysis. Results: The rapid metabolizing probability and non-refractory difference of CYP1A2, CYP3A4 and CYP2D6 genes in patients with refractory schizophrenia were significant. The comparison of fast metabotropic probabilities of CYP1A2, CYP3A4 and CYP2D6 genes in patients treated with 4W after treatment There was no significant difference in the single drug treatment group. The BPRS scale score was significantly higher in the drug-treated group than in the single-drug group. After logistic regression analysis, the refractory characteristics of schizophrenia and The CYP series of genes CYP1A2, CYP3A4, and CYP2D6 are rapidly metabolized. Conclusion: CYP series of genes CYP1A2, CYP3A4, CYP2D6 fast metabolites are related factors of refractory schizophrenia, antipsychotic drugs combined with CYP enzyme inhibitor treatment can improve the efficacy.
Abstract:
Cervical laminoplasty surgery is an alternative to laminectomy. It can increase the space of the spinal cord available by raising the lamina, and achieve the indirect decompression effect to treat cervical spondylosis. Various techniques of laminoplasty have since been developed after two prototype techniques: Hirabayashi’s open-door laminoplasty and Kurokawa’s double-door laminoplasty. Several studies report superior biomechanical stability of the cervical spine after laminoplasty compared with laminectomy. Recent development in laminoplasty is preservation of muscle attachment, which enabled dynamic and static stabilization of the cervical spine by neck extensor muscles. After treatment with new laminoplasty techniques with active postoperative neck muscle exercises, postoperative instability, kyphosis, axial neck pain, and loss of ROM become minimal. The development，complications and future trends of cervical laminoplasty are summarized below.
Cervical laminoplasty surgery is an alternative to laminectomy. It can increase the space of the spinal cord available by raising the lamina, and achieve the indirect decompression effect to treat cervical spondylosis. Various techniques of laminoplasty have since been developed after two prototype techniques: Hirabayashi’s open-door laminoplasty and Kurokawa’s double-door laminoplasty. Several studies report superior biomechanical stability of the cervical spine after laminoplasty compared with laminectomy. Recent development in laminoplasty is preservation of muscle attachment, which enabled dynamic and static stabilization of the cervical spine by neck extensor muscles. After treatment with new laminoplasty techniques with active postoperative neck muscle exercises, postoperative instability, kyphosis, axial neck pain, and loss of ROM become minimal. The development，complications and future trends of cervical laminoplasty are summarized below.
Abstract:
Objective: To investigate the effects of gastrodin on neurotransmitter effect, stress response, blood vessel and nerve function in migraine patients. Methods: A total of 90 patients with migraine admitted to our hospital from September 2016 to December 2017 were randomly divided into observation group and control group with 45 cases in each group. The control group was treated with flunarizine hydrochloride, while the observation group was treated with gastrodin based on the control group. The levels of neurotransmitter effect[including 5-serotonin (5-HT) and tumor necrosis factor alpha (TNF-alpha)], stress response[oxidized low density lipoprotein (ox-LDL) and paraoxonase-1 (PON-1)], vascular function[including nitric oxide (NO), endothelin-1 (ET-1)] and neurological function[including high brain-derived neurotrophic factor (BDNF) and calcitonin gene-related peptide (CGRP)] related indexes were observed and compared between the two groups. Results: The two groups showed significant changes in transmitter effect, stress response, blood vessel and nerve function. Before treatment, there was no significant difference in the levels between the two groups. After treatment, the levels of TNF-a, ox-LDL, NO, ET-1, BDNF and CGRP in the two groups were significantly lower than those before treatment, while the levels of 5-HT and PON-1 were significantly higher than those before treatment. And after treatment, the levels of TNF-a, ox- LDL, NO, ET-1, BDNF and CGRP in the observation group were significantly lower than those in the control group, while the levels of 5-HT and PON-1 were significantly higher than those in the control group. Conclusions: Gastrodin treatment can regulate oxidative stress level and improve vascular and neurological function in migraine patients.
Objective: To investigate the effects of gastrodin on neurotransmitter effect, stress response, blood vessel and nerve function in migraine patients. Methods: A total of 90 patients with migraine admitted to our hospital from September 2016 to December 2017 were randomly divided into observation group and control group with 45 cases in each group. The control group was treated with flunarizine hydrochloride, while the observation group was treated with gastrodin based on the control group. The levels of neurotransmitter effect[including 5-serotonin (5-HT) and tumor necrosis factor alpha (TNF-alpha)], stress response[oxidized low density lipoprotein (ox-LDL) and paraoxonase-1 (PON-1)], vascular function[including nitric oxide (NO), endothelin-1 (ET-1)] and neurological function[including high brain-derived neurotrophic factor (BDNF) and calcitonin gene-related peptide (CGRP)] related indexes were observed and compared between the two groups. Results: The two groups showed significant changes in transmitter effect, stress response, blood vessel and nerve function. Before treatment, there was no significant difference in the levels between the two groups. After treatment, the levels of TNF-a, ox-LDL, NO, ET-1, BDNF and CGRP in the two groups were significantly lower than those before treatment, while the levels of 5-HT and PON-1 were significantly higher than those before treatment. And after treatment, the levels of TNF-a, ox- LDL, NO, ET-1, BDNF and CGRP in the observation group were significantly lower than those in the control group, while the levels of 5-HT and PON-1 were significantly higher than those in the control group. Conclusions: Gastrodin treatment can regulate oxidative stress level and improve vascular and neurological function in migraine patients.
Abstract:
Objective: To observe the effect of Hegu neediling method combined with tendon-stretching and plucking massage on trigger point in the treatment of acute thoracodorsal myofascitis. Methods: A total of 84 patients with acute thoracodorsal myofascitis who met the diagnostic and inclusion criteria were assigned into the treatment group and the control group randomly, the treatment group was treated with Hegu neediling method combined with tendonstretching and plucking massage, while the control group was treated with conventional electroacupuncture. Two groups of patients were treated for two weeks. VAS score and ODI were compared before treatment, after the first treatment and after the treatment course, and the clinical efficacy was evaluated after treatment. Results: The total effective rates of the treatment group and the control group were 97.62% and 80.95%, respectively. The treatment group was significantly better than the control group. The VAS and ODI scores of the treatment group decreased after the first treatment and the end of the treatment, and the treatment group was significantly better than the control group. Conclusion: Hegu neediling method combined with tendon-stretching and plucking massage can relieve pain and improve limb dysfunction in patients with acute thoracodorsal myofascitis.
Objective: To observe the effect of Hegu neediling method combined with tendon-stretching and plucking massage on trigger point in the treatment of acute thoracodorsal myofascitis. Methods: A total of 84 patients with acute thoracodorsal myofascitis who met the diagnostic and inclusion criteria were assigned into the treatment group and the control group randomly, the treatment group was treated with Hegu neediling method combined with tendonstretching and plucking massage, while the control group was treated with conventional electroacupuncture. Two groups of patients were treated for two weeks. VAS score and ODI were compared before treatment, after the first treatment and after the treatment course, and the clinical efficacy was evaluated after treatment. Results: The total effective rates of the treatment group and the control group were 97.62% and 80.95%, respectively. The treatment group was significantly better than the control group. The VAS and ODI scores of the treatment group decreased after the first treatment and the end of the treatment, and the treatment group was significantly better than the control group. Conclusion: Hegu neediling method combined with tendon-stretching and plucking massage can relieve pain and improve limb dysfunction in patients with acute thoracodorsal myofascitis.
Abstract:
Objective: To investigate the correlation between the change in expression of the tumor associated macrophage (TAM) markers CD68 and CD206 and the clinicopathological parameters. Methods: We retrospectively analyzed 28 PGI-DLBCL patients by immunohistochemical stainning. The surface expression of biomarkers CD68 and CD206 on TAMs in samples obtained at diagnosis was compared with that in specimens obtained at first relapse. Based on the change in expression levels of CD68 and CD206 from diagnosis to relapse, patients were categorized into three groups: “increased”, “stable” and “decreased”. Results: A significant difference was observed between the three groups with respect to CD68 and CD206 expressions (P=0.012) and there existed a consistency between the two (P=0.008). The three groups showed a negative correlation with age, sex, stage, B symptoms, diagnosis, ECOG performance status, and IPI scores, while a positive correlation was observed with the site of lesion of CD206 expression (P=0.032). The time to relapse (TTR) in the three groups of CD68 and CD206 was not significantly different (P=0.23, P=0.818). Conclusion: Our study suggests that increase in CD206 and decrease in CD68 expression at relapse indicates M1-TAM polarization into M2-TAM, which plays an important role in PGI-DLBCL relapse. Furthermore, M2-TAM polarization may be associated with the site of Primary gastric diffuse large B lymphoma.
Objective: To investigate the correlation between the change in expression of the tumor associated macrophage (TAM) markers CD68 and CD206 and the clinicopathological parameters. Methods: We retrospectively analyzed 28 PGI-DLBCL patients by immunohistochemical stainning. The surface expression of biomarkers CD68 and CD206 on TAMs in samples obtained at diagnosis was compared with that in specimens obtained at first relapse. Based on the change in expression levels of CD68 and CD206 from diagnosis to relapse, patients were categorized into three groups: “increased”, “stable” and “decreased”. Results: A significant difference was observed between the three groups with respect to CD68 and CD206 expressions (P=0.012) and there existed a consistency between the two (P=0.008). The three groups showed a negative correlation with age, sex, stage, B symptoms, diagnosis, ECOG performance status, and IPI scores, while a positive correlation was observed with the site of lesion of CD206 expression (P=0.032). The time to relapse (TTR) in the three groups of CD68 and CD206 was not significantly different (P=0.23, P=0.818). Conclusion: Our study suggests that increase in CD206 and decrease in CD68 expression at relapse indicates M1-TAM polarization into M2-TAM, which plays an important role in PGI-DLBCL relapse. Furthermore, M2-TAM polarization may be associated with the site of Primary gastric diffuse large B lymphoma.
ZHAO Feng-ting, WANG Peng-bo, WANG Bing, ZHANG Ning, XUE Zhao, SUN Zhi-bin, YUAN Wen-zheng, JI Guo-jie, FENG Hui-gen, HU Huan-huan
2019(11):805-809, DOI: 10.13210/j.cnki.jhmu.20190404.002
Abstract:
Objective: The study is to investigate the regulation of Biochanin A on proliferation and migration of cervical cancer cells and its possible molecular mechanism. Methods: Hela cells were cultured in vitro and treated with different concentrations of Biochanin A. CCK-8 assay was used to detect the effect of Biochanin A on the proliferation of cervical cancer cells, while the Scratch assay was used to detect the effect on cell migration. Besides, the expression of Bcl-2 /Bax mRNA was detected by RT-PCR. Results: Biochanin A could inhibit the proliferation and migration of Hela cells in a dose-dependent and time-dependent manner. At 10,0,40,0,160 μmol/L of Biochanin A, the inhibition rate of Hela cells increased significantly after 24,8 h, significantly higher than that of the control group. The expression of pro-apoptotic protein Bax increased, while the expression of anti-apoptotic protein Bcl-2 decreased in a dose-dependent manner. Conclusion: Hummus A can inhibit the proliferation and migration of Hela cells in cervical cancer, and its mechanism may be related to the down-regulation of Bcl-2 and up-regulation of Bax.
Objective: The study is to investigate the regulation of Biochanin A on proliferation and migration of cervical cancer cells and its possible molecular mechanism. Methods: Hela cells were cultured in vitro and treated with different concentrations of Biochanin A. CCK-8 assay was used to detect the effect of Biochanin A on the proliferation of cervical cancer cells, while the Scratch assay was used to detect the effect on cell migration. Besides, the expression of Bcl-2 /Bax mRNA was detected by RT-PCR. Results: Biochanin A could inhibit the proliferation and migration of Hela cells in a dose-dependent and time-dependent manner. At 10,0,40,0,160 μmol/L of Biochanin A, the inhibition rate of Hela cells increased significantly after 24,8 h, significantly higher than that of the control group. The expression of pro-apoptotic protein Bax increased, while the expression of anti-apoptotic protein Bcl-2 decreased in a dose-dependent manner. Conclusion: Hummus A can inhibit the proliferation and migration of Hela cells in cervical cancer, and its mechanism may be related to the down-regulation of Bcl-2 and up-regulation of Bax.
Abstract:
Objective: To explore the effect of selenium supplementation on thyroid function, immune response and oxidative stress in HT patients with normal thyroid function. Methods: A total of 120 Hashimoto's thyroiditis patients with normal thyroid function admitted to our hospital from June 2016 to March 2018 were randomly divided into treatment group and control group with 60 cases in each group. The control group was given oral placebo, while the treatment group was given selenium yeast. The expression levels of thyroid function [including thyroid stimulating hormone (TSH), free triiodothyronine (FT3) and free thyroxine (FT4)], immune response [including thyroid peroxidase antibody (TPOAb), thyroglobulin antibody (TGAb), interferon-γ (IFN-γ), interleukin-4 (IL-4)] and oxidative stress [including malondialdehyde (MDA), glutathione peroxidase (GPx) and superoxide dismutase (SOD)] were observed and compared between the two groups. Results: The two groups showed significant changes in auxiliary diagnosis, immune response and oxidative stress. Before treatment, there was no significant difference in the levels between the two groups. After treatment, there was no significant difference in the above indexes between the control group and that before treatment. There was no significant difference in thyroid function related indexes between the treatment group and the control group. The levels of TPOAb, TGAb, IFN-γ and MDA in the treatment group were significantly lower than those before treatment and in the control group, while the levels of IL-4, GPx and SOD were significantly higher than those before treatment and in the control group. Conclusions: Selenium supplementation can stabilize thyroid function, reduce the level of thyroid antibody production, and improve immune response and oxidative stress response in HT patients.
Objective: To explore the effect of selenium supplementation on thyroid function, immune response and oxidative stress in HT patients with normal thyroid function. Methods: A total of 120 Hashimoto's thyroiditis patients with normal thyroid function admitted to our hospital from June 2016 to March 2018 were randomly divided into treatment group and control group with 60 cases in each group. The control group was given oral placebo, while the treatment group was given selenium yeast. The expression levels of thyroid function [including thyroid stimulating hormone (TSH), free triiodothyronine (FT3) and free thyroxine (FT4)], immune response [including thyroid peroxidase antibody (TPOAb), thyroglobulin antibody (TGAb), interferon-γ (IFN-γ), interleukin-4 (IL-4)] and oxidative stress [including malondialdehyde (MDA), glutathione peroxidase (GPx) and superoxide dismutase (SOD)] were observed and compared between the two groups. Results: The two groups showed significant changes in auxiliary diagnosis, immune response and oxidative stress. Before treatment, there was no significant difference in the levels between the two groups. After treatment, there was no significant difference in the above indexes between the control group and that before treatment. There was no significant difference in thyroid function related indexes between the treatment group and the control group. The levels of TPOAb, TGAb, IFN-γ and MDA in the treatment group were significantly lower than those before treatment and in the control group, while the levels of IL-4, GPx and SOD were significantly higher than those before treatment and in the control group. Conclusions: Selenium supplementation can stabilize thyroid function, reduce the level of thyroid antibody production, and improve immune response and oxidative stress response in HT patients.
Abstract:
Objective: To study the effect of shexiangbaoxin pill combined with tirofiban hydrochloride on related factors in elderly patients with acute coronary symdrome. Methods: A total of 96 elderly patients with acute coronary symdrome in our hospital from January 2015 to May 2018 were enrolled in this study. The subjects were divided into the control group (n=48) and the treatment group (n=48) randomly. The control group were treated with tirofiban hydrochloride, the treatment group were treated with shexiangbaoxin pill combined with tirofiban hydrochloride, and both the two groups were treated for 2 weeks. The PT, TT, APTT and serum FIB, CK-MB, cTnT, vWF, ET-1, NO, sICAM-1, MMP-9, hs-CRP of the two groups before and after treatment were compared. Results: There were no significantly differences of the PT, TT, APTT and serum FIB, CK-MB, cTnT, vWF, ET-1, NO, sICAM-1, MMP-9, hs-CRP of the two groups before treatment. The PT, TT, APTT and serum NO of the two groups after treatment were significantly higher than before treatment, the serum FIB, vWF, ET, sICAM-1, MMP-9, hs-CRP of the two groups after treatment were significantly lower than before treatment, and that of the treatment group after treatment were significantly better than the control group. The serum CK-MB, cTnT of the two groups after treatment were significantly higher than before treatment, but that of the treatment group after treatment were significantly lower than the control group. Conclusion: Shexiangbaoxin pill combined with tirofiban hydrochloride can significantly improve the blood coagulation function and reduce the Myocardial injury, vascular endothelial damage, inflammation of the elderly patients with acute coronary symdrome, and it was worthy clinical application.
Objective: To study the effect of shexiangbaoxin pill combined with tirofiban hydrochloride on related factors in elderly patients with acute coronary symdrome. Methods: A total of 96 elderly patients with acute coronary symdrome in our hospital from January 2015 to May 2018 were enrolled in this study. The subjects were divided into the control group (n=48) and the treatment group (n=48) randomly. The control group were treated with tirofiban hydrochloride, the treatment group were treated with shexiangbaoxin pill combined with tirofiban hydrochloride, and both the two groups were treated for 2 weeks. The PT, TT, APTT and serum FIB, CK-MB, cTnT, vWF, ET-1, NO, sICAM-1, MMP-9, hs-CRP of the two groups before and after treatment were compared. Results: There were no significantly differences of the PT, TT, APTT and serum FIB, CK-MB, cTnT, vWF, ET-1, NO, sICAM-1, MMP-9, hs-CRP of the two groups before treatment. The PT, TT, APTT and serum NO of the two groups after treatment were significantly higher than before treatment, the serum FIB, vWF, ET, sICAM-1, MMP-9, hs-CRP of the two groups after treatment were significantly lower than before treatment, and that of the treatment group after treatment were significantly better than the control group. The serum CK-MB, cTnT of the two groups after treatment were significantly higher than before treatment, but that of the treatment group after treatment were significantly lower than the control group. Conclusion: Shexiangbaoxin pill combined with tirofiban hydrochloride can significantly improve the blood coagulation function and reduce the Myocardial injury, vascular endothelial damage, inflammation of the elderly patients with acute coronary symdrome, and it was worthy clinical application.
Qiong Huang, Tian Dai, Chen-Di Dong, Ding-Jun Zhang, Bo Liu, Min Zhang, Zeng-Xin Yang, Li Luo, Bing-Qing, Xia1, Yi-Fan Wu
Abstract:
Objective: To systematically evaluate the effects of sleep status on renal function in patients with chronic kidney disease (CKD). Methods: To search the relevant literature related to the effects of sleep status on renal function of CKD patients on PubMed database, EMBase database, the Cochrane Library database, CNKI database, Chinese Biomedical Literature Database, VIP and Wanfang database from the initial to June 2018, all literature that met the criteria were included. According to the type of studies, the quality of the literature was evaluated by NOS scale in the cohort study and AHRQ scale in the cross-sectional study, and systematically evaluated the outcome indicators, the main outcome indicators were estimated glomerular filtration rate (eGFR) and endogenous creatinine clearance rate (Ccr), while the secondary indicators were Pittsburgh Sleep Quality Index (PSQI), Sleep Quality (SQ), Serum Creatinine (Scr), Hemoglobin (Hb), Albumin (ALB) and Urine Protein/Creatinine Ratio (UPCR). Results: Four literature and one meeting abstract were included in this study, of which four were cohort studies, three of them the NOS quality evaluations were high, one of them was medium, the remaining one was cross-sectional study, and the AHRQ quality evaluation was medium. This study shows that sleep status has a certain correlation with renal function. Shorter sleep time or poor sleep quality can lead to deterioration of renal function. Among them, the research data of Sabbatinit research team in Italy showed that Ccr gradually decreased with the increased of the PSQI; studies of Cohen research team and the Ricardo research team in the United States showed that eGFR decreased with the increased of the PSQI; the study of Kumar research team in the United States showed that the lower SQ , the worse renal function; the study of Knutson’ research team in British showed that the shorter sleep time, the lower eGFR. In addition, studies showed that sleep index also has influence on Hb, ALB, Scr, UPCR and other indicators. Conclusion: Sleep status can affect the renal function of CKD patients in different degrees. Shorter sleep time and poor sleep quality will damage renal function and accelerate the progress of CKD.
Objective: To systematically evaluate the effects of sleep status on renal function in patients with chronic kidney disease (CKD). Methods: To search the relevant literature related to the effects of sleep status on renal function of CKD patients on PubMed database, EMBase database, the Cochrane Library database, CNKI database, Chinese Biomedical Literature Database, VIP and Wanfang database from the initial to June 2018, all literature that met the criteria were included. According to the type of studies, the quality of the literature was evaluated by NOS scale in the cohort study and AHRQ scale in the cross-sectional study, and systematically evaluated the outcome indicators, the main outcome indicators were estimated glomerular filtration rate (eGFR) and endogenous creatinine clearance rate (Ccr), while the secondary indicators were Pittsburgh Sleep Quality Index (PSQI), Sleep Quality (SQ), Serum Creatinine (Scr), Hemoglobin (Hb), Albumin (ALB) and Urine Protein/Creatinine Ratio (UPCR). Results: Four literature and one meeting abstract were included in this study, of which four were cohort studies, three of them the NOS quality evaluations were high, one of them was medium, the remaining one was cross-sectional study, and the AHRQ quality evaluation was medium. This study shows that sleep status has a certain correlation with renal function. Shorter sleep time or poor sleep quality can lead to deterioration of renal function. Among them, the research data of Sabbatinit research team in Italy showed that Ccr gradually decreased with the increased of the PSQI; studies of Cohen research team and the Ricardo research team in the United States showed that eGFR decreased with the increased of the PSQI; the study of Kumar research team in the United States showed that the lower SQ , the worse renal function; the study of Knutson’ research team in British showed that the shorter sleep time, the lower eGFR. In addition, studies showed that sleep index also has influence on Hb, ALB, Scr, UPCR and other indicators. Conclusion: Sleep status can affect the renal function of CKD patients in different degrees. Shorter sleep time and poor sleep quality will damage renal function and accelerate the progress of CKD.
2019(11):815-817, 825, DOI: 10.13210/j.cnki.jhmu.20190409.003
Abstract:
Objective: To observe the Levels of serum CORT, ACTH and CRH in SHR rats, the expression of GR, MR and CRH mRNA in hypothalamus and GR, MR mRNA in hippocampus of the ADHD model animal SHR rat, and to explore the HPA axis function and related factors in SHR rats. Methods: Junior SHR rats were used as ADHD model group and WKY rats as control group. The levels of CORT, ACTH and CRH in serum were detected by ELISA, and the mRNA expressions of GR, MR, CRH in hypothalamus and GR, MR in hippocampus were detected by RT-PCR. Results: The levels of serum CORT and ACTH in SHR rats were significantly lower than those in WKY rats (P<0.01), and the levels of serum CRH were significantly higher than those in WKY rats (P<0.01). GR and MR mRNA expression levels in hypothalamus and hippocampus were significantly higher than those in WKY rats (P<0.01), while CRH mRNA expression in hypothalamus was significantly lower than that in WKY rats (P<0.01). Conclusion: The HPA axis of SHR rats showed hyporesponsiveness, which may be related to CRH priming disorder and high expression of GR and MR mRNA in hypothalamus and hippocampus.
Objective: To observe the Levels of serum CORT, ACTH and CRH in SHR rats, the expression of GR, MR and CRH mRNA in hypothalamus and GR, MR mRNA in hippocampus of the ADHD model animal SHR rat, and to explore the HPA axis function and related factors in SHR rats. Methods: Junior SHR rats were used as ADHD model group and WKY rats as control group. The levels of CORT, ACTH and CRH in serum were detected by ELISA, and the mRNA expressions of GR, MR, CRH in hypothalamus and GR, MR in hippocampus were detected by RT-PCR. Results: The levels of serum CORT and ACTH in SHR rats were significantly lower than those in WKY rats (P<0.01), and the levels of serum CRH were significantly higher than those in WKY rats (P<0.01). GR and MR mRNA expression levels in hypothalamus and hippocampus were significantly higher than those in WKY rats (P<0.01), while CRH mRNA expression in hypothalamus was significantly lower than that in WKY rats (P<0.01). Conclusion: The HPA axis of SHR rats showed hyporesponsiveness, which may be related to CRH priming disorder and high expression of GR and MR mRNA in hypothalamus and hippocampus.
2019(11):810-814, DOI: 10.13210/j.cnki.jhmu.20190404.003
Abstract:
Objective: To investigate the effects of ivabradine on Notch and NF-kappa B signaling pathway in myocardial cells of rats with myocardial infarction. Methods: The model of myocardial infarction was established by ligating the left anterior descending coronary artery. The surviving rats were randomly divided into model group (MI group, n=8) and treatment group (IVA group, n=8). Rats with the same location but without ligation of the left anterior descending coronary artery were used as control group (CON group, n=8). IVA was administered for 28 d. Hemodynamic and cardiac function indexes of all rats were measured:heart rate (HR), systolic pressure (SBP), diastolic pressure (DBP), mean arterial pressure (MAP), left ventricular systolic pressure (LVSP), left ventricular end-diastolic pressure (LVEDP) and maximum rate of increase and decrease of left ventricular internal pressure (+dp/dt); left ventricular mass index, left ventricular cross-sectional diameter and infarct area; The expression of Notch signaling pathway components (Notch-1, Dll-4, Hes-1) in rat cardiomyocytes was detected by PT-PCR, and the expression of DICD-1 and P65 protein was detected by western-blot. One-way ANOVA was used for comparison between groups, and SNK was used for comparison between groups. Results: SBP, DBP, MAP, LASP, LVEDP and (+dp/dt) in MI group were lower than those in control group (P<0.05), while IVA was higher than those in MI group (P<0.05). Left ventricular mass index and left ventricular sectional diameter in MI group were significantly higher than those in control group (P<0.05), but lower than those in IVA group (P<0.05). The expression of Notch-1 in MI group was significantly higher than that in control group (P<0.05), but lower than that in IVA group (P<0.05). There was no significant difference in the expression of Dll-4 and Hes-1 between the three groups (P>0.05). The expression levels of NICD-1 and P 65 in MI group were significantly higher than those in CON group (P<0.05), but lower than those in IVA group (P<0.05). Conclusion: IVA may improve cardiac function and inhibit ventricular remodeling in rats with myocardial infarction through Notch and NF-kappa B signaling pathways.
Objective: To investigate the effects of ivabradine on Notch and NF-kappa B signaling pathway in myocardial cells of rats with myocardial infarction. Methods: The model of myocardial infarction was established by ligating the left anterior descending coronary artery. The surviving rats were randomly divided into model group (MI group, n=8) and treatment group (IVA group, n=8). Rats with the same location but without ligation of the left anterior descending coronary artery were used as control group (CON group, n=8). IVA was administered for 28 d. Hemodynamic and cardiac function indexes of all rats were measured:heart rate (HR), systolic pressure (SBP), diastolic pressure (DBP), mean arterial pressure (MAP), left ventricular systolic pressure (LVSP), left ventricular end-diastolic pressure (LVEDP) and maximum rate of increase and decrease of left ventricular internal pressure (+dp/dt); left ventricular mass index, left ventricular cross-sectional diameter and infarct area; The expression of Notch signaling pathway components (Notch-1, Dll-4, Hes-1) in rat cardiomyocytes was detected by PT-PCR, and the expression of DICD-1 and P65 protein was detected by western-blot. One-way ANOVA was used for comparison between groups, and SNK was used for comparison between groups. Results: SBP, DBP, MAP, LASP, LVEDP and (+dp/dt) in MI group were lower than those in control group (P<0.05), while IVA was higher than those in MI group (P<0.05). Left ventricular mass index and left ventricular sectional diameter in MI group were significantly higher than those in control group (P<0.05), but lower than those in IVA group (P<0.05). The expression of Notch-1 in MI group was significantly higher than that in control group (P<0.05), but lower than that in IVA group (P<0.05). There was no significant difference in the expression of Dll-4 and Hes-1 between the three groups (P>0.05). The expression levels of NICD-1 and P 65 in MI group were significantly higher than those in CON group (P<0.05), but lower than those in IVA group (P<0.05). Conclusion: IVA may improve cardiac function and inhibit ventricular remodeling in rats with myocardial infarction through Notch and NF-kappa B signaling pathways.
2019(11):829-833, DOI: 10.13210/j.cnki.jhmu.20190426.003
Abstract:
Objective: To explore the effect of dopamine neurotransmitter expression on sleep disorders in neurological inpatients. Methods: 34 patients with sleep disorders and 30 volunteers from February 2018 to November 2018 in neurology department of our hospital were selected as the study subjects. They were the observation group and the control group. 64 participants stopped taking stimulating drinks such as coffee and alcohol two weeks ago. The sleep quality and depression were measured by Pittsburgh Sleep Quality Index (PSQI) and Hamilton Depression Scale (HAMD). Degree of depression was examined by brain MRI. Two days later, 11C-Raclopril labeled brain dopamine neurotransmitter binding to receptors was scanned by PET/CT. SPSS was used to analyze the correlation between dopamine neurotransmitters and sleep disorders. Results: The HAMD and PSQI scores of the observation group were significantly higher than those of the control group (P<0.05). On PET/CT scans, 11C-Raclopride was mostly located in nucleus dorsal caudate putamen, caudate nucleus and putamen. The dopamine binding ability of nucleus dorsal caudate putamen, caudate nucleus and putamen in the observation group was significantly lower than that in the control group (P< 0.05). Pearson correlation analysis showed that the course of disease in the observation group was negatively correlated with nucleus dorsal caudate putamen, caudate nucleus and putamen (r=-0.754,-0.717,-0.728;P< 0.05), while the scores of HAMD and PSQI were not correlated with nucleus dorsal caudate putamen, caudate nucleus and putamen (P> 0.05). Conclusion: The decrease of dopamine receptor binding in patients with sleep disorders is related to the course of sleep disorders, suggesting that the decrease of dopamine expression and sleep disorders have clinical significance.
Objective: To explore the effect of dopamine neurotransmitter expression on sleep disorders in neurological inpatients. Methods: 34 patients with sleep disorders and 30 volunteers from February 2018 to November 2018 in neurology department of our hospital were selected as the study subjects. They were the observation group and the control group. 64 participants stopped taking stimulating drinks such as coffee and alcohol two weeks ago. The sleep quality and depression were measured by Pittsburgh Sleep Quality Index (PSQI) and Hamilton Depression Scale (HAMD). Degree of depression was examined by brain MRI. Two days later, 11C-Raclopril labeled brain dopamine neurotransmitter binding to receptors was scanned by PET/CT. SPSS was used to analyze the correlation between dopamine neurotransmitters and sleep disorders. Results: The HAMD and PSQI scores of the observation group were significantly higher than those of the control group (P<0.05). On PET/CT scans, 11C-Raclopride was mostly located in nucleus dorsal caudate putamen, caudate nucleus and putamen. The dopamine binding ability of nucleus dorsal caudate putamen, caudate nucleus and putamen in the observation group was significantly lower than that in the control group (P< 0.05). Pearson correlation analysis showed that the course of disease in the observation group was negatively correlated with nucleus dorsal caudate putamen, caudate nucleus and putamen (r=-0.754,-0.717,-0.728;P< 0.05), while the scores of HAMD and PSQI were not correlated with nucleus dorsal caudate putamen, caudate nucleus and putamen (P> 0.05). Conclusion: The decrease of dopamine receptor binding in patients with sleep disorders is related to the course of sleep disorders, suggesting that the decrease of dopamine expression and sleep disorders have clinical significance.
Abstract:
Objective: To investigate the effect of dexmedetomidine against liver ischemia-reperfusion injury in rats by reducing endoplasmic reticulum stress. Methods: A total of 24 adult male Sprague-Dawley rats were randomly divided into sham operation group (6 rats, Sham group), liver ischemia-reperfusion injury group (6 rats, I/R group), and liver ischemia-reperfusion injury +dexmedetomidine pretreatment group (6 rats, I/R +Dex pretreatment group) (25 μg/kg intraperitoneally 30 min before ischemia), and liver ischemia-reperfusion injury +dexmedetomidine post-treatment group (6 rats, I/R +Dex post-treatment group) (25 μg/kg intraperitoneally 30 min after reperfusion). Hepatic ischemia-reperfusion injury model was performed by after clamping the hepatic hilum for 30 min and reperfusion for 6 h. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were measured by automatic biochemical analyzer. Malondialdehyde (MDA) and superoxide dismutase (SOD) activity were detected by enzyme-linked immunosorbent assay (ELISA). The pathological changes of each group were observed by HE staining. The expression of endoplasmic reticulum stress (P-PERK, P-IRE1α, CHOP) were detected by Western blot. Results: Compared with the Sham group, the serum levels of ALT and AST were significantly higher in the I/R group, compared with the I/R group, the serum ALT and AST in I/R+Dex pretreatment group and the I/R+Dex post-treatment group were significantly reduced; Compared with the Sham group, MDA activity in the liver tissue of the I/R group was significantly increased, while the SOD activity was significantly decreased, and the pathological score of the liver tissue was significantly increased; Compared with the I/R group, MDA activity and liver histopathology scores in I/R+Dex pretreatment group and the I/R+Dex post-treatment group were decreased, while SOD activity increased; The expression of P-PERK, P-IRE1α, CHOP in the I/R group were significantly higher than that in the Sham group, while the expression of the above indicators were reduced in I/R+Dex pretreatment group and the I/R+Dex post-treatment group. Conclusion: Dexmedetomidine can significantly attenuated liver ischemia-reperfusion injury in rats, which may be related to the reduction of endoplasmic reticulum stress.
Objective: To investigate the effect of dexmedetomidine against liver ischemia-reperfusion injury in rats by reducing endoplasmic reticulum stress. Methods: A total of 24 adult male Sprague-Dawley rats were randomly divided into sham operation group (6 rats, Sham group), liver ischemia-reperfusion injury group (6 rats, I/R group), and liver ischemia-reperfusion injury +dexmedetomidine pretreatment group (6 rats, I/R +Dex pretreatment group) (25 μg/kg intraperitoneally 30 min before ischemia), and liver ischemia-reperfusion injury +dexmedetomidine post-treatment group (6 rats, I/R +Dex post-treatment group) (25 μg/kg intraperitoneally 30 min after reperfusion). Hepatic ischemia-reperfusion injury model was performed by after clamping the hepatic hilum for 30 min and reperfusion for 6 h. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were measured by automatic biochemical analyzer. Malondialdehyde (MDA) and superoxide dismutase (SOD) activity were detected by enzyme-linked immunosorbent assay (ELISA). The pathological changes of each group were observed by HE staining. The expression of endoplasmic reticulum stress (P-PERK, P-IRE1α, CHOP) were detected by Western blot. Results: Compared with the Sham group, the serum levels of ALT and AST were significantly higher in the I/R group, compared with the I/R group, the serum ALT and AST in I/R+Dex pretreatment group and the I/R+Dex post-treatment group were significantly reduced; Compared with the Sham group, MDA activity in the liver tissue of the I/R group was significantly increased, while the SOD activity was significantly decreased, and the pathological score of the liver tissue was significantly increased; Compared with the I/R group, MDA activity and liver histopathology scores in I/R+Dex pretreatment group and the I/R+Dex post-treatment group were decreased, while SOD activity increased; The expression of P-PERK, P-IRE1α, CHOP in the I/R group were significantly higher than that in the Sham group, while the expression of the above indicators were reduced in I/R+Dex pretreatment group and the I/R+Dex post-treatment group. Conclusion: Dexmedetomidine can significantly attenuated liver ischemia-reperfusion injury in rats, which may be related to the reduction of endoplasmic reticulum stress.
Abstract:
Objective: To explore the best time for progesterone supplementation in AID ovulation induction cycles by Letrozole. Methods: The data analysed in this study were collected from 509 patients who were performed AID (Artificial Insemination by Donor) administrated letrozole (LE) between 2014.8-2015.7. All patients were randomly divided into 4 groups by the time of progesterone administrated, including experimental group and the control group. The experimental group was divided into group 1-72 h after ovulation, group 2-48 h after ovulation, group 3-24 h after ovulation and control group—without administrated LE. The gestation and live birth rate were evaluated by monitoring vaginal ultrasound and HCG blood value 14 d after AID. Results: The pregnancy rate with administrated progesterone added 72 h after ovulation was 31.9%, which was significantly higher than those of other groups, the same situation as groups added progesterone was significantly higher than the control group. However, there was no significant difference in the numbers of abortions among the four groups. The LBR of group 4 was significantly lower than that of group 1. Conclutions: Progesterone administrated 72 h after ovulation can promoted the gestation rate, but did not affect the rate of miscarrage .
Objective: To explore the best time for progesterone supplementation in AID ovulation induction cycles by Letrozole. Methods: The data analysed in this study were collected from 509 patients who were performed AID (Artificial Insemination by Donor) administrated letrozole (LE) between 2014.8-2015.7. All patients were randomly divided into 4 groups by the time of progesterone administrated, including experimental group and the control group. The experimental group was divided into group 1-72 h after ovulation, group 2-48 h after ovulation, group 3-24 h after ovulation and control group—without administrated LE. The gestation and live birth rate were evaluated by monitoring vaginal ultrasound and HCG blood value 14 d after AID. Results: The pregnancy rate with administrated progesterone added 72 h after ovulation was 31.9%, which was significantly higher than those of other groups, the same situation as groups added progesterone was significantly higher than the control group. However, there was no significant difference in the numbers of abortions among the four groups. The LBR of group 4 was significantly lower than that of group 1. Conclutions: Progesterone administrated 72 h after ovulation can promoted the gestation rate, but did not affect the rate of miscarrage .
Abstract:
Objective: To study the expression and significance of KAI1/CD82, p27 proteins in gastric carcinoma. Methods: The expressions of KAI1/CD82, p27 proteins were detected by immunohistchemistry S-P method in 58 cases of gastric carcinoma tissues and 23 normal gastric tissues. Results: The positive rates of KAI1/CD82 and p27 proteins in gastric carcinoma tissues were 29.3% and 34.4%, but 90% and 85% respectively in normal gastric tissue. And there is a significant difference between the two groups. The expression level of KAI1/CD82 and p27 proteins was significantly related to tumor invasive depth, grade of tumor differentiation, the clinical stage and lymph node metastasis, And has nothing to do with age or gender. There was a positive correlation between the expression of KAI1/CD82 and P27 in gastric cancer tissues. Conclusion: The expression of KAI1/CD82 and p27 protein may be involved in the initiation and development in gastric carcinoma, and the combined detection of KAI1/CD82 and p27 proteins may be significant in predicting the invision and metastasis of gastric carcinoma.
Objective: To study the expression and significance of KAI1/CD82, p27 proteins in gastric carcinoma. Methods: The expressions of KAI1/CD82, p27 proteins were detected by immunohistchemistry S-P method in 58 cases of gastric carcinoma tissues and 23 normal gastric tissues. Results: The positive rates of KAI1/CD82 and p27 proteins in gastric carcinoma tissues were 29.3% and 34.4%, but 90% and 85% respectively in normal gastric tissue. And there is a significant difference between the two groups. The expression level of KAI1/CD82 and p27 proteins was significantly related to tumor invasive depth, grade of tumor differentiation, the clinical stage and lymph node metastasis, And has nothing to do with age or gender. There was a positive correlation between the expression of KAI1/CD82 and P27 in gastric cancer tissues. Conclusion: The expression of KAI1/CD82 and p27 protein may be involved in the initiation and development in gastric carcinoma, and the combined detection of KAI1/CD82 and p27 proteins may be significant in predicting the invision and metastasis of gastric carcinoma.
2019(11):801-804, 809, DOI: 10.13210/j.cnki.jhmu.20190416.002
Abstract:
Objective: To investigate the mechanism of treating chronic wounds in rats by using Zhuang medicine Choerospondias axillaris bark. Methods: The whole layer skin defection 40 rats were used and randomly divided into 4 groups:normal group, model group, MEBO group, choerospondias group. The normal group and model group were given external deposited normal saline external application. MEBO group used MEBT/MEBO. Choerospondias group was given external deposited choerospondias axillaris bark water decoction external application. Detected cell ultrastructure, PTEN, VEGF mRNA after 12 d. Results: The organelle structure in MEBO group and choerospondias group were restored. In 12 d, PTEN in MEBO group and choerospondias group were lower, VEGF were higher than 1d (P<0.05). PTEN in MEBO group and choerospondias group were lower, VEGF were higher than model group (P<0.05). MEBO group had no difference with choerospondias group (P>0.05). Conclusion: Choerospondias axillaris bark water decoction could remarkably improve the cell ultrastructure in chronic wounds, regulate the PTEN signal conduction effect, and promote wound healing.
Objective: To investigate the mechanism of treating chronic wounds in rats by using Zhuang medicine Choerospondias axillaris bark. Methods: The whole layer skin defection 40 rats were used and randomly divided into 4 groups:normal group, model group, MEBO group, choerospondias group. The normal group and model group were given external deposited normal saline external application. MEBO group used MEBT/MEBO. Choerospondias group was given external deposited choerospondias axillaris bark water decoction external application. Detected cell ultrastructure, PTEN, VEGF mRNA after 12 d. Results: The organelle structure in MEBO group and choerospondias group were restored. In 12 d, PTEN in MEBO group and choerospondias group were lower, VEGF were higher than 1d (P<0.05). PTEN in MEBO group and choerospondias group were lower, VEGF were higher than model group (P<0.05). MEBO group had no difference with choerospondias group (P>0.05). Conclusion: Choerospondias axillaris bark water decoction could remarkably improve the cell ultrastructure in chronic wounds, regulate the PTEN signal conduction effect, and promote wound healing.
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