Journal of Hainan Medical University
  • Current Issue
  • Online First
  • Archive
  • Most Downloaded
    Select AllDeselectExport
    Display Method:
    2022,28(16):1201-1206, DOI: 10.13210/j.cnki.jhmu.20220729.001
    Abstract:
    Novel coronavirus (SARS‑CoV‑2, SARS‑CoV‑2 for short) infection can cause a series of gastrointestinal damage. Related studies have reported that SARS‑CoV‑2 infection can lead to the occurrence and progression of ulcerative colitis , which may be related to the cytokine storm caused by SARS‑CoV‑2 infection. Recently, we have also paid attention to whether infection with SARS‑CoV‑2 will aggravate the condition of UC patients receiving biological immunotherapy and whether vaccination with SARS‑CoV‑2 is safe and effective for these patients. At present, the interaction mechanism between SARS‑CoV‑2, SARS‑CoV‑2 vaccine and ulcerative colitis is not fully understood, and more research is needed to further clarify the relationship.
    2022,28(16):1207-1213, DOI: 10.13210/j.cnki.jhmu.20220525.002
    Abstract:
    Objective: To explore the effects of Hainan papaya extract on learning and memory impairment and anti‑aging in D‑galactose‑induced aging mice. Methods:A total of 72 Kunming mice with normal cognitive ability screened by water maze test were randomly divided into negative control group, model group, piracetam group, high, medium and low dose groups of Hainan papaya extract (400 mg/kg,200 mg/kg ,100 mg/kg), with 12 mice in each group. Hainan papaya extract and piracetam group were given the above drugs by gavage every day, The negative control and model groups were given the same amount of 0.9% NaCl solution in the same way. Mice in each group were weighed once a week; At the same time, except for the negative control group, mice in each group were intraperitoneally injected with 2% D‑galactose every day, and the negative control group was intraperitoneally injected with normal saline for 7 weeks.After 7 weeks, We observed each group of mice’s capacity of learning and memory by Morris water maze behavioral test; Then, the content of superoxide dismutase (SOD), malondialdehyde (MDA),catalase (CAT), and nitric oxide synthase (NOS) were measured; On the other hand, we observed the hippocampus’ histopathological changes by hematoxylin‑eosin staining, and measured the protein expression of nuclear factor‑E2‑related factor (Nrf2) in brain tissue of mice in each group by Western blot. Results: After the intervention of Hainan papaya extract on aging model mice, the high, medium and low dose groups could shorten the swimming time and swimming distance of mice to varying degrees, increase the activities of SOD, CAT and NOS in mouse brain tissue and reduce the content of MDA, The performance of high dose group was better than piracetam group (P<0.01). At the same time, it can improve the histopathological changes of neurons in mouse hippocampus by reducing neuronal nuclear pyknosis, and increase the expression of Nrf2 protein in mouse brain in a dose‑dependent manner.Conclusion: Hainan papaya extract is able to postpone various physical signs of subacute aging mice caused by D‑galactose, and possesses definite anti‑aging and antioxidant effects, which may be related to the regulation of Nrf2 signal pathway.
    2022,28(16):1214-1221, DOI: 10.13210/j.cnki.jhmu.20220609.001
    Abstract:
    Objective: To investigate the effect and mechanism of Hai Tongpi decoction ointment on analgesic and anti⁃inflammatory effects in mice after transdermal administration. Methods: The hot plate method and the acetic acid writhing experiment were used to verify the analgesic effect of Hai tong⁃pi extract on pain model mice; the xylene⁃induced auricular swelling model and the carrageenan⁃induced right foot swelling model were used, combined with the ELISA method to determine the analgesic effect. The levels of TNF⁃α, IL⁃1β and IL⁃6 in the serum of mice were observed. Anti⁃inflammatory effect of Haitongpi Tang decoction ointment on inflammation model mice. Results: In terms of analgesia, compared with the model group, both the treatment group 1 and the treatment group 2 can significantly prolong the hot plate pain threshold and the latency of the writhing response (P<0.05), and significantly reduce the writhing in the mice. The number of times (P<0.001), and the effect of process 1 is more obvious (P<0.05); In terms of anti⁃inflammatory, compared with the blank group, the swelling degree and swelling rate of the right hind paw of the mice in the model group were significantly increased (P<0.01), indicating that the acute inflammation model was successfully established in the mice. Compared with the model group, both the process group 1 and the process group 2 can significantly reduce the swelling degree and swelling rate of the mice feet (P<0.05), and reduce the auricle swelling degree of the mice, and increase the swelling inhibition rate (P<0.05), and the effective effect of process 1 group was more significant (P<0.05). At the same time, compared with the blank group, the contents of TNF⁃α, IL⁃1β and IL⁃6 in the model group were significantly increased (P<0.01). Compared with the process group 2, the process group 1 had a more significant decrease in the content of IL⁃1β (P<0.05), and IL⁃6 content of the two had no statistical difference (P>0.05). Conclusion: The Hai tong⁃pi decoction ointment prepared by two different processes have good analgesic and anti⁃inflammatory effects on mice after transdermal administration. inflammatory cytokine expression. The anti⁃inflammatory and analgesic effect of process group 1 was better than that of process group 2, which may be related to the separate extraction and storage of volatile oil of traditional Chinese medicine, which ensured the long⁃term pharmacological activity of volatile oil.
    2022,28(16):1222-1227, DOI: 10.13210/j.cnki.jhmu.20220530.002
    Abstract:
    Objective: To explore the relationship between the changes of miR145‑5p/Smads pathway and macrophage polarization in adjuvant arthritis rats. Methods: Twelve rats were divided into normal group and model group induced by freund's complete adjuvant (0.1 mL/mouse) by random number table method, with 6 rats in each group. The expression of inflammatory polarization markers IL‑8 and CD206 in synovial tissue was detected by enzyme‑linked immunosorbent assay on the 12th day after the formation of arthritis in rats. Western blotting was used to detect the expression of TGF‑β1/Smads pathway factors in synovial tissues.The expression of miR145‑5P, Smads3 and Smads7 in synovial tissue was detected by RT‑qPCR. Results: Compared with normal group, the expression levels of IL‑8, TGF‑β1 and Smad3 in model group were significantly increased (P<0.05); The expression levels of CD206, Smad7 and miR145‑5P were significantly decreased (P<0.01). The correlation results showed that IL‑8 was positively correlated with Smad3 (P<0.01), IL‑8 was negatively correlated with Smad7 (P<0.05), CD206 was negatively correlated with Smad3 (P<0.01) and positively correlated with Smad7 (P<0.05). miR145‑5p was negatively correlated with Smad3 (P<0.01) and positively correlated with Smad7 (P<0.01). Conclusion: miR145‑5p may inhibit the overactivation of TGF‑β1/Smads pathway, regulate macrophage polarization, and inhibit the development of adjuvant arthritis by inhibiting Smad3 expression.
    2022,28(16):1228-1231, DOI: 10.13210/j.cnki.jhmu.20220622.001
    Abstract:
    Objective: To study and evaluate the clini1cal effect of plasma exchange in the adjuvant treatment of chronic and acute liver failure. Methods:Totally 60 patients with chronic and acute liver failure in our department were divided into two groups. The control group received comprehensive treatment and the plasma exchange group received plasma exchange. The changes of total bilirubin(TBIL) ,PT percentage activity(PTA), alanine aminotransferase(ALT), aspartate aminotransferase(AST), albumin(ALB), interleukin-6(IL-6), procalcitonin(PCT) and C-reactive protein (CRP) were measured after treatment, and were assessed in terms of the degree of relief of clinical symptoms.Results: TBIL, ALB, Alt, AST, IL-6, PCT and CRP in the two groups decreased and PTA increased compared with those before treatment, but the curative effect of the treatment group was better than that of the control group (P<0.05).The total effective rate of the treatment group was 80.0%, which was higher than 46.6% of the treatment group (P<0.05).Conclusion: Plasma exchange can help to improve the liver function, remove a large number of inflammatory factors, promote the recovery of liver function and improve the prognosis of patients with chronic and acute liver failure.
    2022,28(16):1232-1241, DOI: 10.13210/j.cnki.jhmu.20210917.001
    Abstract:
    Objective: To systematically evaluate the clinical efficacy and safety of Hanchuan Zupa granule(HCZPG) in the treatment of bronchial asthma. Methods: The Cochrane Library, Pubmed, EMBASE, CNKI, CBM, VIP and Wangfang were searched up to June 2021. Randomized controlled trials, which assessed the effects of HCZPG in the treatment of bronchial asthma, were included in this review, and the references included in the selected studies were also tracked. The methodological quality of eligible studies were assessed by the Cochrane Collaboration’s tool. Data of the included studies were extracted independently by two authors and meta-analysis was conducted using RevMan5.3. Results: A total of 97 relevant literatures were retrieved, and 13 studies were finally included, involving 1 189 patients. The aggregated meta-analysis results indicated that HCZPG improved total clinical efficacy [RR=1.18,95% CI(1.13,1.24),P<0.000 01], FEV1(L)[WMD=0.48,95% CI (0.22,0.74),P =0.000 3], FVC(L)[WMD=1.23,95% CI (0.5,1.96),P=0.001], FEV1/FVC(%) [WMD=7.37,95% CI (5.91,8.84),P<0.000 01], PEF [SMD)=1.19,95% CI (0.82,1.56),P<0.000 01], IL-4 [WMD=-12.13,95%CI (-16.36,-7.90),P<0.000 01], IL-6 [WMD=-29.55,95% CI (-40.71,-18.39),P<0.000 01], TNF-α[SMD=-2.73,95%CI (-3.57,-1.88),P<0.000 01]、PaO2WMD=8.97,95% CI (6.09,11.85),P<0.000 01], but the risk of adverse events were not statistically significant between the two groups [RR=0.68, 95%CI (0.39,1.18),P=0.17]. Conclusion: HCZPG shows good clinical curative effects in treating bronchial asthma without increasing the incidence of adverse events.
    2022,28(16):1252-1260, DOI: 10.13210/j.cnki.jhmu.20210511.002
    Abstract:
    Objective: To analyse the key compounds, targets and pathways of the treatment of non‑alcoholic fatty liver disease (NAFLD) by Jianwei Gexia Zhuyu Decoction based on network pharmacology, in order to explore the molecular mechanism of its therapeutic effects.Methods: The differential genes between sick and normal conditions were screened by GEO‑Datasets, and the heat map and volcano map were drawn. The active compounds in Jianwei Gexia Zhuyu Decoction were searched by TCMSP platform and Drugbank database. OB≥30% and DL≥0.18 were set as thresholds to screen potential active compounds and action targets. The molecular target maps of Jianwei Gexia Zhuyu Decoction and NAFLD differential genes were constructed, and the PPI network and network topology parameters were obtained by STRING database. The PPI network and network topology parameters were visually analyzed by Cytoscape, and the core regulatory genes were screened. At the same time, the SwissDock platform was used to dock the main active components with the target. The main pathways were determined by GO biological function enrichment analysis and KEGG metabolic pathway enrichment analysis by DAVID.Results: After screening, 377 differential genes (127 up‑regulated genes and 250 down‑regulated genes), 225 active compounds of Jianwei Gexia Zhuyu Decoction, 308 corresponding targets were obtained; 14 key targets were screened, corresponding to 168 compounds, and the key targets involved MYC, FOSL2, FOS, etc. The results of GO functional enrichment analysis showed that Jianwei Gexia Zhuyu Decoction mainly regulated the activity expression of DNA binding transcriptional activator and the specific transcription of RNA polymeraseⅡ; The results of molecular docking showed that the main active components quercetin and baicalein had good binding activity with VCAM1, HSPB1, MYC, JUN and so on; The results of KEGG enrichment analysis showed that it was mainly involved in IL‑17 signal pathway, Wnt receptor signal pathway, NF‑κB signal pathway, TNF signal pathway and AGE‑RAGE signal pathway in diabetic complications.Conclusion: Through the interaction of multi‑components and multi‑targets, Jianwei Gexia Zhuyu Decoction has achieved the goal of overall treatment of NAFLD from many ways. The application of network pharmacology provides a new research approach and scientific basis for further study on the mechanism of Jianwei Gexia Zhuyu Decoction in the treatment of NAFLD.
    2022,28(16):1261-1267, DOI: 10.13210/j.cnki.jhmu.20210506.001
    Abstract:
    Objective: To analyze the prescription and medication rule of traditional Chinese medicine(TCM) in treating cardiac neurosis by using the software of "Traditional Chinese Medicine Inheritance Assistant Platform (V2.5)". Methods: China Journal Full-Text Database, Wanfang database, Weipu Chinese Periodical Service Platform and Chinese biomedical literature database were retrieved according to the inclusion and exclusion criteria. Literatures on the treatment of cardiac neurosis with TCM prescriptions were screened out and data mining was carried out using the TCM inheritance assistant platform. Use frequency, commonly used dose, the frequency of properties, flavors and meridian distribution were counted. Association rules and entropy clustering analysis for complex system were used to extract core drug combinations and unsupervised entropy hierarchical clustering was used to predict potential new prescriptions. Results: Through literature retrieval and screening, 210 types of prescriptions were finally selected, and 181 types of Chinese herbs were extracted, with cumulative frequency of 2 410 times. Among them, there were 46 traditional Chinese medicines that were used more than 15 times. According to the efficacy and indication, they were classified into qi - regulating drugs, tranquilizers, qi - tonifying drugs, heat - clearing drugs, blood - tonifying drugs, etc. According to properties, flavors, and meridian distribution, warm and sweet drugs were the most commonly used, mostly distributing to the heart meridian, followed by liver and spleen meridians. Association rules showed that combination of radix paeoniae and paeonia-bupleurum was the most used; faructus aurantii and bupleurum had the strongest correlation, up to 90%. The network diagram indicated that bupleurum, angelica sinensis, radix paeoniae radix, and poria cocos were the core of the association network. Based on complex system entropy clustering, 24 core drug combinations were obtained. The unsupervised entropy hierarchical clustering algorithm was used to extract 12 core drug combinations, and 6 potential new formulae were obtained by further clustering. Conclusion: Regulating qi and blood, addressing both symptoms and causes,and using purgation and tonification at the same time is the principle of the treatment of cardiac neuropathy with TCM. The treatment mainly starts from the heart, liver and spleen, and therapies include soothing the liver and regulating qi, clearing heat and resolving phlegm, tranquilizing and calming the nerves,and prompting blood circulation and diuresis.
    2022,28(16):1268-1274, DOI: 10.13210/j.cnki.jhmu.20210507.002
    Abstract:
    Myocardial ischemia/reperfusion (I/R) injury is a pathological change that occurs during the restoration of blood supply to ischemic or occluded coronary arteries after coronary heart disease, stroke, cardiac arrest and resuscitation, organ transplantation, shock and other events. Myocardial I/R injury is often accompanied by cardiovascular adverse events, which seriously affect the prognosis of myocardial ischemia. The potential mechanism of myocardial I/R injury is complex, involving many pathological processes, such as oxygen free radical injury, calcium overload, inflammation, apoptosis, activation, immune imbalance, endoplasmic reticulum stress, autophagy, myocardial energy metabolism disorder, myocardial microvascular endothelial cell injury and so on. Ischemic preconditioning is an effective preventive and therapeutic measure to reduce ischemia-reperfusion (I/R) injury. At present, there are many experimental studies on the pathogenesis and prevention and treatment measures, but the clinical application is still limited, so the prevention and treatment of I/R injury is still a major challenge. The pathogenesis of myocardial I/R injury is not completely clear, and the treatment methods and drugs are limited, so this paper summarizes the molecular mechanism and related signal pathways involved in its injury, as well as the emerging targeted therapy, to provide strategy and theoretical basis for clinical prevention and treatment of myocardial I/R injury.
    2022,28(16):1275-1280, DOI: 10.13210/j.cnki.jhmu.20210419.004
    Abstract:
    Disorder of energy metabolism is a major pathological change in the progression of heart failure. This process leads to insufficient myocardial energy and further aggravates cardiac dysfunction. Disorders of metabolic substrate utilization, mainly glucose and fatty acids, play an important role in this process. Research over the years has shown that some traditional Chinese medicines or compound prescriptions whose main role is to replenish qi and warm yang have good effects in regulating energy metabolism disorders. It has been found that some active ingredients in traditional Chinese medicine can regulate the uptake and utilization of myocardial cell metabolic substrate, so that the metabolism of myocardial cells can be adjusted in a direction that is beneficial to the body under hypoxic conditions, increasing the overall energy supply of the myocardium and improving heart function. This article reviewed the research of traditional Chinese medicine intervention on glucose and lipid metabolism of heart failure myocardial cells, and preliminarily summarizes the law and mechanism of traditional Chinese medicine intervention in heart failure myocardial glucose and fatty acid metabolism, hoping to provide clues for energy metabolism therapy research from the perspective of traditional Chinese medicine.
    Select AllDeselectExport
    Display Method:
    Select AllDeselectExport
    Display Method:
    2019,25(5):6-9, DOI:
    Abstract:
    Objective: To study the effect of Baihe Dihuang Decoction in improving anxiety and its mechanism of action. Methods: Male mice were randomly divided into 5 groups: blank control group, Baihe Dihuang Decoction high, medium, low-dose group and diazepam group. After continuous intragastric administration for 14 d, the behavioral test of the mouse elevated plus maze experiment and opening test was performed. The brain tissue GABA and Glu content was measured by immunosorbent assay (ELISA). Results: Compared with the blank control group, the middle and high doses of Baihe Dihuang Decoction could increase the time and frequency of movement of the mice in the open cross labyrinth in the open arm and increase the number of times that the mouse entered the central area in the opening test. The anti-anxiety effect also showed an increase in the brain tissue GABA content in mice, ignificantly decreased Glu contents in mice. Conclusion: Baihe Dihuang Decoction has some anxiolytic effect, and antianxiety effect may be related to increasing brain tissue GABA content, decreased Glu contents in mice.
    2019,25(5):1-5, DOI:
    Abstract:
    Objective: The current study aims to identify the effects of exogenous application of pulsed electromagnetic fields on skin wound healing in diabetic rats, and thus provides experimental evidence for its more scientific clinical application in the future. Methods: The diabetic animal models were established via intraperitoneal injection of streptozotocin in 4-monthold male rats. Fourth eight rats were randomly assigned into the diabetes group (DM) and diabetes coupled with pulsed electromagnetic fields (DM+PEMF) group. Another 24 normal rats were used as the blank control group (Control). Then, all the rats in the three groups were subjected to dorsal surgery for the establishment of soft tissue wound model (circular wound with the diameter of 2 cm). Then, the rats in the DM+PEMF group were subjected to pulsed electromagnetic fields stimulation. Then, 6 rats in each group were sacrificed at Day 5, 12 and 19 post surgery. The glucose levels, wound closure, wound healing time and tissue tensile strength were examined and analyzed. Results: Pulsed electromagnetic fields significantly increased the wound closure rate in diabetic rats at Day 5, 12 and 19 post surgery, decreased overall wound healing period in diabetic rats, and also enhanced tissue tensile strength in diabetic rats at Day 5, 12 and 19 post surgery. Conclusion: Low-intensity pulsed electromagnetic fields can significantly accelerate diabetic wound healing process, and also improve diabetic tissue repair capacity. This study may be helpful for providing more scientific and reasonable experimental evidence for the treatment of pulsed electromagnetic fields on diabetic wound healing in clinics.
    2019,25(5):69-72, DOI:
    Abstract:
    Gastric cancer is one of common malignant tumors in clinic. It has high incidence, high mortality and poor prognosis. Generally, gastric cancer is divided into early gastric cancer (EGC) and advanced gastric cancer. The 5-year survival rate of EGC is higher than that of advanced gastric cancer. In recent years, with the continuous development of science and technology, a variety of medical instruments and technologies have been updated, greatly promoting the detection rate of EGC, reducing the rate of EGC missed diagnosis. This article reviews the progress of EGC endoscopic diagnosis.
    2019,25(5):61-64, DOI:
    Abstract:
    Objective: To investigate the effects of routine rehabilitation therapy combined with low frequency head stimulation on monoamine neurotransmitters, neuron-specific enolase (NSE), endothelin-1 (ET-1) and cerebral hemodynamics in children with cerebral palsy. Methods: From January 2017 to June 2018, 110 children with cerebral palsy were randomly divided into observation group (55 cases) and control group (55 cases). The control group received routine rehabilitation treatment, while the observation group received low-frequency head stimulation on the basis of routine rehabilitation treatment. The changes of dopamine (DA), norepinephrine (NE), serotonin (5-HT), NSE, ET-1 levels and mean blood flow velocity of anterior cerebral artery (ACA), middle cerebral artery (MCA), posterior cerebral artery (PCA) were compared in two groups. Results: Before treatment, there was no significant difference in DA, NE and 5-HT levels in two groups. After treatment, DA, NE and 5-HT levels in the observation group were (192.23±22.71) ng/mL, (98.02±11.71) ng/L, (210.07±25.03) ng/L, and in the control group. the DA, NE, 5-HT levels were (147.06±17.02) ng/mL, (83.07±11.15) ng/L, and (171.88±20.45) ng/L, respectively. The DA, NE and 5-HT levels in two groups were higher than those before treatment, and DA, NE and 5-HT levels in the observation group were higher than those in the control group. Before treatment, there was no significant difference in NSE and ET-1 levels between the two groups. After treatment, the NSE and ET-1 levels in the observation group were (7.97±2.07) μg/L and (41.01±10.07) pg/ mL, and the NSE and ET-1 levels in the control group were (10.38±3.02) μg/L, (58.46±15.02) pg/ mL, respectively. the NSE and ET-1 in two groups were lower than those before treatment, and the NSE and ET-1 of the observation group were lower than the control group. Before treatment, there was no significant difference in mean blood flow velocity between ACA, MCA and PCA. After treatment, the mean blood flow velocities of ACA, MCA, and PCA in the observation group were (46.88±7.72) cm/s, (59.85±10.18) cm/s, and (49.15±7.02) cm/s, respectively, which was significantly higher than before treatment and higher than that of the control group in the same period. Conclusion: Conventional rehabilitation combined with low-frequency electrical stimulation of the head can effectively increase the content of monoamine neurotransmitters in children with cerebral palsy, enhance cerebral blood circulation, and reduce brain damage
    2019,25(5):65-68, DOI:
    Abstract:
    Objective: To study the refractory factors associated with schizophrenia. Methods: 200 patients with refractory schizophrenia and 200 patients with non-refractory schizophrenia were selected. The CYP series of genes CYP1A2, CYP3A4 and CYP2D6 were detected by drug gene, and the rapid metabolic probabilities of the three genes were compared and analyzed. 200 patients with refractory schizophrenia were randomly divided into two groups: the combined drug treatment group and the single drug treatment group. The results were compared between the treatment of 0W and 4W for drug gene detection, 3 genes fast metabolizing type and BPRS scale. analysis. Results: The rapid metabolizing probability and non-refractory difference of CYP1A2, CYP3A4 and CYP2D6 genes in patients with refractory schizophrenia were significant. The comparison of fast metabotropic probabilities of CYP1A2, CYP3A4 and CYP2D6 genes in patients treated with 4W after treatment There was no significant difference in the single drug treatment group. The BPRS scale score was significantly higher in the drug-treated group than in the single-drug group. After logistic regression analysis, the refractory characteristics of schizophrenia and The CYP series of genes CYP1A2, CYP3A4, and CYP2D6 are rapidly metabolized. Conclusion: CYP series of genes CYP1A2, CYP3A4, CYP2D6 fast metabolites are related factors of refractory schizophrenia, antipsychotic drugs combined with CYP enzyme inhibitor treatment can improve the efficacy.
    2019,25(5):73-76, DOI:
    Abstract:
    Cervical laminoplasty surgery is an alternative to laminectomy. It can increase the space of the spinal cord available by raising the lamina, and achieve the indirect decompression effect to treat cervical spondylosis. Various techniques of laminoplasty have since been developed after two prototype techniques: Hirabayashi’s open-door laminoplasty and Kurokawa’s double-door laminoplasty. Several studies report superior biomechanical stability of the cervical spine after laminoplasty compared with laminectomy. Recent development in laminoplasty is preservation of muscle attachment, which enabled dynamic and static stabilization of the cervical spine by neck extensor muscles. After treatment with new laminoplasty techniques with active postoperative neck muscle exercises, postoperative instability, kyphosis, axial neck pain, and loss of ROM become minimal. The development,complications and future trends of cervical laminoplasty are summarized below.
    2019,25(5):41-44, DOI:
    Abstract:
    Objective: To investigate the effects of gastrodin on neurotransmitter effect, stress response, blood vessel and nerve function in migraine patients. Methods: A total of 90 patients with migraine admitted to our hospital from September 2016 to December 2017 were randomly divided into observation group and control group with 45 cases in each group. The control group was treated with flunarizine hydrochloride, while the observation group was treated with gastrodin based on the control group. The levels of neurotransmitter effect[including 5-serotonin (5-HT) and tumor necrosis factor alpha (TNF-alpha)], stress response[oxidized low density lipoprotein (ox-LDL) and paraoxonase-1 (PON-1)], vascular function[including nitric oxide (NO), endothelin-1 (ET-1)] and neurological function[including high brain-derived neurotrophic factor (BDNF) and calcitonin gene-related peptide (CGRP)] related indexes were observed and compared between the two groups. Results: The two groups showed significant changes in transmitter effect, stress response, blood vessel and nerve function. Before treatment, there was no significant difference in the levels between the two groups. After treatment, the levels of TNF-a, ox-LDL, NO, ET-1, BDNF and CGRP in the two groups were significantly lower than those before treatment, while the levels of 5-HT and PON-1 were significantly higher than those before treatment. And after treatment, the levels of TNF-a, ox- LDL, NO, ET-1, BDNF and CGRP in the observation group were significantly lower than those in the control group, while the levels of 5-HT and PON-1 were significantly higher than those in the control group. Conclusions: Gastrodin treatment can regulate oxidative stress level and improve vascular and neurological function in migraine patients.
    2019,25(5):49-52, DOI:
    Abstract:
    Objective: To observe the effect of Hegu neediling method combined with tendon-stretching and plucking massage on trigger point in the treatment of acute thoracodorsal myofascitis. Methods: A total of 84 patients with acute thoracodorsal myofascitis who met the diagnostic and inclusion criteria were assigned into the treatment group and the control group randomly, the treatment group was treated with Hegu neediling method combined with tendonstretching and plucking massage, while the control group was treated with conventional electroacupuncture. Two groups of patients were treated for two weeks. VAS score and ODI were compared before treatment, after the first treatment and after the treatment course, and the clinical efficacy was evaluated after treatment. Results: The total effective rates of the treatment group and the control group were 97.62% and 80.95%, respectively. The treatment group was significantly better than the control group. The VAS and ODI scores of the treatment group decreased after the first treatment and the end of the treatment, and the treatment group was significantly better than the control group. Conclusion: Hegu neediling method combined with tendon-stretching and plucking massage can relieve pain and improve limb dysfunction in patients with acute thoracodorsal myofascitis.
    2019,25(5):26-30, DOI:
    Abstract:
    Objective: To investigate the correlation between the change in expression of the tumor associated macrophage (TAM) markers CD68 and CD206 and the clinicopathological parameters. Methods: We retrospectively analyzed 28 PGI-DLBCL patients by immunohistochemical stainning. The surface expression of biomarkers CD68 and CD206 on TAMs in samples obtained at diagnosis was compared with that in specimens obtained at first relapse. Based on the change in expression levels of CD68 and CD206 from diagnosis to relapse, patients were categorized into three groups: “increased”, “stable” and “decreased”. Results: A significant difference was observed between the three groups with respect to CD68 and CD206 expressions (P=0.012) and there existed a consistency between the two (P=0.008). The three groups showed a negative correlation with age, sex, stage, B symptoms, diagnosis, ECOG performance status, and IPI scores, while a positive correlation was observed with the site of lesion of CD206 expression (P=0.032). The time to relapse (TTR) in the three groups of CD68 and CD206 was not significantly different (P=0.23, P=0.818). Conclusion: Our study suggests that increase in CD206 and decrease in CD68 expression at relapse indicates M1-TAM polarization into M2-TAM, which plays an important role in PGI-DLBCL relapse. Furthermore, M2-TAM polarization may be associated with the site of Primary gastric diffuse large B lymphoma.
    2019(11):805-809, DOI: 10.13210/j.cnki.jhmu.20190404.002
    Abstract:
    Objective: The study is to investigate the regulation of Biochanin A on proliferation and migration of cervical cancer cells and its possible molecular mechanism. Methods: Hela cells were cultured in vitro and treated with different concentrations of Biochanin A. CCK-8 assay was used to detect the effect of Biochanin A on the proliferation of cervical cancer cells, while the Scratch assay was used to detect the effect on cell migration. Besides, the expression of Bcl-2 /Bax mRNA was detected by RT-PCR. Results: Biochanin A could inhibit the proliferation and migration of Hela cells in a dose-dependent and time-dependent manner. At 10,0,40,0,160 μmol/L of Biochanin A, the inhibition rate of Hela cells increased significantly after 24,8 h, significantly higher than that of the control group. The expression of pro-apoptotic protein Bax increased, while the expression of anti-apoptotic protein Bcl-2 decreased in a dose-dependent manner. Conclusion: Hummus A can inhibit the proliferation and migration of Hela cells in cervical cancer, and its mechanism may be related to the down-regulation of Bcl-2 and up-regulation of Bax.
    2019,25(5):45-48, DOI:
    Abstract:
    Objective: To explore the effect of selenium supplementation on thyroid function, immune response and oxidative stress in HT patients with normal thyroid function. Methods: A total of 120 Hashimoto's thyroiditis patients with normal thyroid function admitted to our hospital from June 2016 to March 2018 were randomly divided into treatment group and control group with 60 cases in each group. The control group was given oral placebo, while the treatment group was given selenium yeast. The expression levels of thyroid function [including thyroid stimulating hormone (TSH), free triiodothyronine (FT3) and free thyroxine (FT4)], immune response [including thyroid peroxidase antibody (TPOAb), thyroglobulin antibody (TGAb), interferon-γ (IFN-γ), interleukin-4 (IL-4)] and oxidative stress [including malondialdehyde (MDA), glutathione peroxidase (GPx) and superoxide dismutase (SOD)] were observed and compared between the two groups. Results: The two groups showed significant changes in auxiliary diagnosis, immune response and oxidative stress. Before treatment, there was no significant difference in the levels between the two groups. After treatment, there was no significant difference in the above indexes between the control group and that before treatment. There was no significant difference in thyroid function related indexes between the treatment group and the control group. The levels of TPOAb, TGAb, IFN-γ and MDA in the treatment group were significantly lower than those before treatment and in the control group, while the levels of IL-4, GPx and SOD were significantly higher than those before treatment and in the control group. Conclusions: Selenium supplementation can stabilize thyroid function, reduce the level of thyroid antibody production, and improve immune response and oxidative stress response in HT patients.
    2019,25(5):37-40, DOI:
    Abstract:
    Objective: To study the effect of shexiangbaoxin pill combined with tirofiban hydrochloride on related factors in elderly patients with acute coronary symdrome. Methods: A total of 96 elderly patients with acute coronary symdrome in our hospital from January 2015 to May 2018 were enrolled in this study. The subjects were divided into the control group (n=48) and the treatment group (n=48) randomly. The control group were treated with tirofiban hydrochloride, the treatment group were treated with shexiangbaoxin pill combined with tirofiban hydrochloride, and both the two groups were treated for 2 weeks. The PT, TT, APTT and serum FIB, CK-MB, cTnT, vWF, ET-1, NO, sICAM-1, MMP-9, hs-CRP of the two groups before and after treatment were compared. Results: There were no significantly differences of the PT, TT, APTT and serum FIB, CK-MB, cTnT, vWF, ET-1, NO, sICAM-1, MMP-9, hs-CRP of the two groups before treatment. The PT, TT, APTT and serum NO of the two groups after treatment were significantly higher than before treatment, the serum FIB, vWF, ET, sICAM-1, MMP-9, hs-CRP of the two groups after treatment were significantly lower than before treatment, and that of the treatment group after treatment were significantly better than the control group. The serum CK-MB, cTnT of the two groups after treatment were significantly higher than before treatment, but that of the treatment group after treatment were significantly lower than the control group. Conclusion: Shexiangbaoxin pill combined with tirofiban hydrochloride can significantly improve the blood coagulation function and reduce the Myocardial injury, vascular endothelial damage, inflammation of the elderly patients with acute coronary symdrome, and it was worthy clinical application.
    2019,25(5):31-36, DOI:
    Abstract:
    Objective: To systematically evaluate the effects of sleep status on renal function in patients with chronic kidney disease (CKD). Methods: To search the relevant literature related to the effects of sleep status on renal function of CKD patients on PubMed database, EMBase database, the Cochrane Library database, CNKI database, Chinese Biomedical Literature Database, VIP and Wanfang database from the initial to June 2018, all literature that met the criteria were included. According to the type of studies, the quality of the literature was evaluated by NOS scale in the cohort study and AHRQ scale in the cross-sectional study, and systematically evaluated the outcome indicators, the main outcome indicators were estimated glomerular filtration rate (eGFR) and endogenous creatinine clearance rate (Ccr), while the secondary indicators were Pittsburgh Sleep Quality Index (PSQI), Sleep Quality (SQ), Serum Creatinine (Scr), Hemoglobin (Hb), Albumin (ALB) and Urine Protein/Creatinine Ratio (UPCR). Results: Four literature and one meeting abstract were included in this study, of which four were cohort studies, three of them the NOS quality evaluations were high, one of them was medium, the remaining one was cross-sectional study, and the AHRQ quality evaluation was medium. This study shows that sleep status has a certain correlation with renal function. Shorter sleep time or poor sleep quality can lead to deterioration of renal function. Among them, the research data of Sabbatinit research team in Italy showed that Ccr gradually decreased with the increased of the PSQI; studies of Cohen research team and the Ricardo research team in the United States showed that eGFR decreased with the increased of the PSQI; the study of Kumar research team in the United States showed that the lower SQ , the worse renal function; the study of Knutson’ research team in British showed that the shorter sleep time, the lower eGFR. In addition, studies showed that sleep index also has influence on Hb, ALB, Scr, UPCR and other indicators. Conclusion: Sleep status can affect the renal function of CKD patients in different degrees. Shorter sleep time and poor sleep quality will damage renal function and accelerate the progress of CKD.
    2019(11):815-817, 825, DOI: 10.13210/j.cnki.jhmu.20190409.003
    Abstract:
    Objective: To observe the Levels of serum CORT, ACTH and CRH in SHR rats, the expression of GR, MR and CRH mRNA in hypothalamus and GR, MR mRNA in hippocampus of the ADHD model animal SHR rat, and to explore the HPA axis function and related factors in SHR rats. Methods: Junior SHR rats were used as ADHD model group and WKY rats as control group. The levels of CORT, ACTH and CRH in serum were detected by ELISA, and the mRNA expressions of GR, MR, CRH in hypothalamus and GR, MR in hippocampus were detected by RT-PCR. Results: The levels of serum CORT and ACTH in SHR rats were significantly lower than those in WKY rats (P<0.01), and the levels of serum CRH were significantly higher than those in WKY rats (P<0.01). GR and MR mRNA expression levels in hypothalamus and hippocampus were significantly higher than those in WKY rats (P<0.01), while CRH mRNA expression in hypothalamus was significantly lower than that in WKY rats (P<0.01). Conclusion: The HPA axis of SHR rats showed hyporesponsiveness, which may be related to CRH priming disorder and high expression of GR and MR mRNA in hypothalamus and hippocampus.
    2019(11):810-814, DOI: 10.13210/j.cnki.jhmu.20190404.003
    Abstract:
    Objective: To investigate the effects of ivabradine on Notch and NF-kappa B signaling pathway in myocardial cells of rats with myocardial infarction. Methods: The model of myocardial infarction was established by ligating the left anterior descending coronary artery. The surviving rats were randomly divided into model group (MI group, n=8) and treatment group (IVA group, n=8). Rats with the same location but without ligation of the left anterior descending coronary artery were used as control group (CON group, n=8). IVA was administered for 28 d. Hemodynamic and cardiac function indexes of all rats were measured:heart rate (HR), systolic pressure (SBP), diastolic pressure (DBP), mean arterial pressure (MAP), left ventricular systolic pressure (LVSP), left ventricular end-diastolic pressure (LVEDP) and maximum rate of increase and decrease of left ventricular internal pressure (+dp/dt); left ventricular mass index, left ventricular cross-sectional diameter and infarct area; The expression of Notch signaling pathway components (Notch-1, Dll-4, Hes-1) in rat cardiomyocytes was detected by PT-PCR, and the expression of DICD-1 and P65 protein was detected by western-blot. One-way ANOVA was used for comparison between groups, and SNK was used for comparison between groups. Results: SBP, DBP, MAP, LASP, LVEDP and (+dp/dt) in MI group were lower than those in control group (P<0.05), while IVA was higher than those in MI group (P<0.05). Left ventricular mass index and left ventricular sectional diameter in MI group were significantly higher than those in control group (P<0.05), but lower than those in IVA group (P<0.05). The expression of Notch-1 in MI group was significantly higher than that in control group (P<0.05), but lower than that in IVA group (P<0.05). There was no significant difference in the expression of Dll-4 and Hes-1 between the three groups (P>0.05). The expression levels of NICD-1 and P 65 in MI group were significantly higher than those in CON group (P<0.05), but lower than those in IVA group (P<0.05). Conclusion: IVA may improve cardiac function and inhibit ventricular remodeling in rats with myocardial infarction through Notch and NF-kappa B signaling pathways.
    2019(11):829-833, DOI: 10.13210/j.cnki.jhmu.20190426.003
    Abstract:
    Objective: To explore the effect of dopamine neurotransmitter expression on sleep disorders in neurological inpatients. Methods: 34 patients with sleep disorders and 30 volunteers from February 2018 to November 2018 in neurology department of our hospital were selected as the study subjects. They were the observation group and the control group. 64 participants stopped taking stimulating drinks such as coffee and alcohol two weeks ago. The sleep quality and depression were measured by Pittsburgh Sleep Quality Index (PSQI) and Hamilton Depression Scale (HAMD). Degree of depression was examined by brain MRI. Two days later, 11C-Raclopril labeled brain dopamine neurotransmitter binding to receptors was scanned by PET/CT. SPSS was used to analyze the correlation between dopamine neurotransmitters and sleep disorders. Results: The HAMD and PSQI scores of the observation group were significantly higher than those of the control group (P<0.05). On PET/CT scans, 11C-Raclopride was mostly located in nucleus dorsal caudate putamen, caudate nucleus and putamen. The dopamine binding ability of nucleus dorsal caudate putamen, caudate nucleus and putamen in the observation group was significantly lower than that in the control group (P< 0.05). Pearson correlation analysis showed that the course of disease in the observation group was negatively correlated with nucleus dorsal caudate putamen, caudate nucleus and putamen (r=-0.754,-0.717,-0.728;P< 0.05), while the scores of HAMD and PSQI were not correlated with nucleus dorsal caudate putamen, caudate nucleus and putamen (P> 0.05). Conclusion: The decrease of dopamine receptor binding in patients with sleep disorders is related to the course of sleep disorders, suggesting that the decrease of dopamine expression and sleep disorders have clinical significance.
    2019,25(5):10-13, DOI:
    Abstract:
    Objective: To investigate the effect of dexmedetomidine against liver ischemia-reperfusion injury in rats by reducing endoplasmic reticulum stress. Methods: A total of 24 adult male Sprague-Dawley rats were randomly divided into sham operation group (6 rats, Sham group), liver ischemia-reperfusion injury group (6 rats, I/R group), and liver ischemia-reperfusion injury +dexmedetomidine pretreatment group (6 rats, I/R +Dex pretreatment group) (25 μg/kg intraperitoneally 30 min before ischemia), and liver ischemia-reperfusion injury +dexmedetomidine post-treatment group (6 rats, I/R +Dex post-treatment group) (25 μg/kg intraperitoneally 30 min after reperfusion). Hepatic ischemia-reperfusion injury model was performed by after clamping the hepatic hilum for 30 min and reperfusion for 6 h. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were measured by automatic biochemical analyzer. Malondialdehyde (MDA) and superoxide dismutase (SOD) activity were detected by enzyme-linked immunosorbent assay (ELISA). The pathological changes of each group were observed by HE staining. The expression of endoplasmic reticulum stress (P-PERK, P-IRE1α, CHOP) were detected by Western blot. Results: Compared with the Sham group, the serum levels of ALT and AST were significantly higher in the I/R group, compared with the I/R group, the serum ALT and AST in I/R+Dex pretreatment group and the I/R+Dex post-treatment group were significantly reduced; Compared with the Sham group, MDA activity in the liver tissue of the I/R group was significantly increased, while the SOD activity was significantly decreased, and the pathological score of the liver tissue was significantly increased; Compared with the I/R group, MDA activity and liver histopathology scores in I/R+Dex pretreatment group and the I/R+Dex post-treatment group were decreased, while SOD activity increased; The expression of P-PERK, P-IRE1α, CHOP in the I/R group were significantly higher than that in the Sham group, while the expression of the above indicators were reduced in I/R+Dex pretreatment group and the I/R+Dex post-treatment group. Conclusion: Dexmedetomidine can significantly attenuated liver ischemia-reperfusion injury in rats, which may be related to the reduction of endoplasmic reticulum stress.
    2019,25(5):22-25, DOI:
    Abstract:
    Objective: To explore the best time for progesterone supplementation in AID ovulation induction cycles by Letrozole. Methods: The data analysed in this study were collected from 509 patients who were performed AID (Artificial Insemination by Donor) administrated letrozole (LE) between 2014.8-2015.7. All patients were randomly divided into 4 groups by the time of progesterone administrated, including experimental group and the control group. The experimental group was divided into group 1-72 h after ovulation, group 2-48 h after ovulation, group 3-24 h after ovulation and control group—without administrated LE. The gestation and live birth rate were evaluated by monitoring vaginal ultrasound and HCG blood value 14 d after AID. Results: The pregnancy rate with administrated progesterone added 72 h after ovulation was 31.9%, which was significantly higher than those of other groups, the same situation as groups added progesterone was significantly higher than the control group. However, there was no significant difference in the numbers of abortions among the four groups. The LBR of group 4 was significantly lower than that of group 1. Conclutions: Progesterone administrated 72 h after ovulation can promoted the gestation rate, but did not affect the rate of miscarrage .
    2019,25(5):18-21, DOI:
    Abstract:
    Objective: To study the expression and significance of KAI1/CD82, p27 proteins in gastric carcinoma. Methods: The expressions of KAI1/CD82, p27 proteins were detected by immunohistchemistry S-P method in 58 cases of gastric carcinoma tissues and 23 normal gastric tissues. Results: The positive rates of KAI1/CD82 and p27 proteins in gastric carcinoma tissues were 29.3% and 34.4%, but 90% and 85% respectively in normal gastric tissue. And there is a significant difference between the two groups. The expression level of KAI1/CD82 and p27 proteins was significantly related to tumor invasive depth, grade of tumor differentiation, the clinical stage and lymph node metastasis, And has nothing to do with age or gender. There was a positive correlation between the expression of KAI1/CD82 and P27 in gastric cancer tissues. Conclusion: The expression of KAI1/CD82 and p27 protein may be involved in the initiation and development in gastric carcinoma, and the combined detection of KAI1/CD82 and p27 proteins may be significant in predicting the invision and metastasis of gastric carcinoma.
    2019(11):801-804, 809, DOI: 10.13210/j.cnki.jhmu.20190416.002
    Abstract:
    Objective: To investigate the mechanism of treating chronic wounds in rats by using Zhuang medicine Choerospondias axillaris bark. Methods: The whole layer skin defection 40 rats were used and randomly divided into 4 groups:normal group, model group, MEBO group, choerospondias group. The normal group and model group were given external deposited normal saline external application. MEBO group used MEBT/MEBO. Choerospondias group was given external deposited choerospondias axillaris bark water decoction external application. Detected cell ultrastructure, PTEN, VEGF mRNA after 12 d. Results: The organelle structure in MEBO group and choerospondias group were restored. In 12 d, PTEN in MEBO group and choerospondias group were lower, VEGF were higher than 1d (P<0.05). PTEN in MEBO group and choerospondias group were lower, VEGF were higher than model group (P<0.05). MEBO group had no difference with choerospondias group (P>0.05). Conclusion: Choerospondias axillaris bark water decoction could remarkably improve the cell ultrastructure in chronic wounds, regulate the PTEN signal conduction effect, and promote wound healing.

Publication ethics

WeChat

Mobile website

Close